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Reagent erythrocyte preservation system and preparation method thereof

A technology of reagent red blood cells and red blood cells, which is applied in the medical field and can solve problems that have not been reported

Active Publication Date: 2020-09-29
SHANGHAI BLOOD CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] About a kind of reagent erythrocyte preservation system of the present invention and its preparation method have not yet been reported

Method used

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  • Reagent erythrocyte preservation system and preparation method thereof
  • Reagent erythrocyte preservation system and preparation method thereof
  • Reagent erythrocyte preservation system and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Effect experiment

[0033] Red blood cells are a special kind of cells. Mature red blood cells do not have a nucleus, so that their metabolism depends entirely on their own glycolysis for energy. As the storage time increases, the metabolism of energy substances will cause changes in the structure of the red blood cell membrane. This change in membrane structure will, on the one hand, cause changes in red blood cell membrane permeability and cell morphology; on the other hand, it will also lead to changes in the spatial conformation of membrane proteins, leading to changes in antigenicity. Therefore, this example aims to evaluate the cell membrane structure and antigenicity objectively to reflect the effect of the reagent red blood cell preservation solution on the preservation of the reagent red blood cell.

[0034] 1 Experimental method

[0035] 1.1 Reagent red blood cell preservation solution (reagent red blood cell preservation solution, hereinafter referred to ...

Embodiment 2

[0069] Example 2 Concentration gradient test

[0070] 1 Experimental method

[0071] Prepare respectively 0.7; 0.8; 0.9; 1; 1.2; 1.5X preservation solution (the optimal concentration is 1), and carry out 3% cell ratio and 15% cell ratio preservation experiments. The 7th day and the 75th day of the planned preservation period of 3% and 15% cells were selected as the two detection nodes to evaluate the short-term and long-term effects of the gradient on cells.

[0072] 2 results

[0073] Observation of storage appearance: From the storage appearance of 7 days and 75 days, 1X storage solution has the best storage effect, which is the best concentration (see Figure 5 ).

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Abstract

The invention relates to a reagent erythrocyte preservation system and a preparation method thereof. The reagent erythrocyte preservation system comprises the following components: 0.400 g / L of citricacid-1 water, 6.100 g / L of trisodium citrate dihydrate, 20.500 g / L of glucose, 0.350 g / L of inosine, 0.143 g / L of chloramphenicol, 0.143 g / L of neomycin sulfate, 4.200g / L of NaCl, 0.500 g / L of adenine, 4.000 g / L of mannitol, and 3.000 g / L of potassium dihydrogen phosphate. Experiments prove that when the reagent erythrocyte preservation system is used for storing 3% of reagent erythrocytes at 2-8DEG C, the reagent erythrocytes can be effectively preserved for 90 days, and 15% of the reagent erythrocytes can be effectively preserved for 60 days. The preservation system has the advantages thatthe reagent erythrocytes obtained by using the preservation system are long in effective period, normal in appearance, high in red blood cell form proportion and good in antigen stability.

Description

Technical field [0001] The invention relates to the field of medical technology, in particular to a reagent red blood cell preservation system and a preparation method thereof. Background technique [0002] Red blood cell blood group antigens are usually glycoproteins that penetrate the membrane single or multiple times. In terms of expression, blood group antigen proteins can be divided into expression proteins directly encoded by nuclear genes and adsorption proteins; in relation to cell membrane components, some of these proteins are anchored proteins that constitute the cytoskeleton, and some are linked to anchored proteins. The interaction is relatively stable and bound to the membrane, and some only rely on the interaction with the phospholipid layer to bind to the membrane. Studies have shown that the structure and stability of red blood cell membranes and membrane proteins will change with the increase of storage time. Blood transfusion laboratories usually use reagent r...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0215A01N1/0221A01N1/0226
Inventor 赵俸涌向东郭忠慧朱自严钱呈睿刘李栋
Owner SHANGHAI BLOOD CENT
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