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Colorimetric and surface-enhanced Raman dual-sensing analysis method and reagent for copper ions

A technology of surface enhancement and analysis method, applied in the field of copper ion detection, can solve the problems of poor control of reaction temperature, limited application, strong reduction performance of sodium borohydride, etc., and achieves rapid colorimetric qualitative analysis, detection sensitivity, and sample dosage. less effect

Pending Publication Date: 2020-09-25
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this technology is that the reducing performance of sodium borohydride is too strong and the reaction temperature is not easy to control, and it is difficult to obtain nano-silver materials with controllable morphology and good reproducibility, which limits its application.

Method used

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  • Colorimetric and surface-enhanced Raman dual-sensing analysis method and reagent for copper ions
  • Colorimetric and surface-enhanced Raman dual-sensing analysis method and reagent for copper ions
  • Colorimetric and surface-enhanced Raman dual-sensing analysis method and reagent for copper ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of GNPs-AgNPs structure

[0041] (1) Preparation of gold nanoparticles

[0042] Add 100ml of water and 1ml of 10% HAucl to the three-neck flask 4 Heat to boiling, then add 1ml% sodium citrate, boil for 30min and the solution turns wine red, stop heating, cool to room temperature, filter with 0.45μM microporous membrane, centrifuge at 6000r for 10min three times, concentrate and enrich gold nanoparticles.

[0043] (2) Preparation of 4-mercaptopyridine modified gold nanoparticles

[0044] Take 10ul of 1mM 4-mercaptopyridine ethanol solution and 1ml of gold nanoparticle solution and incubate at room temperature for 6h, and centrifuge at 5000r for 3min to remove unbound 4-mercaptopyridine. The centrifuged precipitate was dispersed with 1 ml of ultrapure water. The obtained 4-mercaptopyridine-modified gold nanorod dispersion was stored at 4°C.

[0045] (3) Preparation of spherical silver nanoparticles

[0046] Slowly add 40ml of glycerin and 60ml of water in...

Embodiment 2

[0052] Cu 2+ Complexation Test of GNPs-AgNPs Probe Molecules

[0053] (1)Cu 2+ Complexation of GNPs-AgNPs probe molecules

[0054] A series of concentrations of copper ions were added to the implementation case 1 to assemble GNPs-Cu 2+ -The color change of the AgNPs system before and after assembly is as follows: figure 1 shown.

[0055] (2) Raman detection of copper ions

[0056] The present invention uses an ultraviolet-visible absorption spectrometer to measure the color change after adding different concentrations of copper ions (0-100μM). figure 2 It is the change of the UV response spectrum of the GNPs-AgNPs probe molecule with the increase of the copper ion concentration in Example 1. figure 2 It is shown that the ultraviolet absorption peaks of AgNPs and GNPs appear at 423nm and 531nm respectively, and as the concentration of copper ions increases, copper ions and probe molecules chelate to make nanoparticles aggregate at 730nm and a new peak appears, image 3...

Embodiment 3

[0060] Actual water and soil sample testing

[0061] Figure 6 Schematically provides a flow chart for the detection of copper ions in water samples and soil samples according to an embodiment of the present invention, the detection method includes the following steps:

[0062] (1) Prepare gold nanoparticles, the steps are the same as in Example 1;

[0063] (2) Prepare gold nanoparticles modified by 4-mercaptopyridine, the steps are the same as in Example 1;

[0064] (3) preparing silver nanoparticles, the steps are the same as in Example 1;

[0065] (4) prepare silver nanoparticles modified with 4-mercaptobenzoic acid, the steps are the same as in Example 1;

[0066] (5) Gold nanoparticles modified by 4-mercaptopyridine and silver nanoparticles modified by 4-mercaptobenzoic acid are mixed, and the steps are the same as in Example 1;

[0067] (6) 4-Mercaptopyridine-modified gold nanoparticles and 4-mercaptobenzoic acid-modified silver nanoparticles are added to the mixed s...

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Abstract

The invention relates to a colorimetric and surface-enhanced Raman dual-sensing analysis method for copper ions. The method comprises the following steps: S1, preparing gold nanoparticles modified by4-mercaptopyridine; S2, preparing silver nanoparticles modified by 4-mercaptobenzoic acid; S3, mixing and incubating the silver nanoparticles modified by 4-mercaptobenzoic acid and the gold nanoparticles modified by 4-mercaptopyridine, and obtaining a GNPs-AgNPs compound; S4, dropwise adding a solution to be tested onto the surface of the GNPs-AgNPs compound; carrying out colorimetric qualitativeanalysis on the standard color, then carrying out qualitative analysis by adopting an ultraviolet-visible absorption spectrometer, simultaneously detecting a Raman signal by adopting a Raman spectrometer, and realizing qualitative and quantitative detection by virtue of spectrum contrast and signal intensity conversion. Compared with the prior art, the GNPs-AgNPs structure prepared by the invention is used for detecting Cu<2+>, and the GNPs-AgNPs structure is combined with Cu<2+> to form a sandwich-like structure, so that the detection is extremely sensitive, the sample consumption is low, andthe detection limit is lower than that of a single probe molecule.

Description

technical field [0001] The invention relates to the field of copper ion detection, in particular to a colorimetric and surface-enhanced Raman dual-sensing analysis method and reagent for copper ions. Background technique [0002] With the improvement of people's quality of life, people's research on environmental pollution and various trace elements in living organisms has become imminent, and reports related to this aspect are also increasing. Cu 2+ It is also a kind of heavy metal ion that widely exists in the environment, and also plays a role in the human body as a trace element to maintain the normal work of the body. This trace element is needed for hematopoiesis in human cells, some enzyme reactions and some life substances. The redox process also requires the participation of copper ions. If Cu in the living body 2+ If the metabolism is destroyed, it may lead to some diseases, such as Wilson's disease, Alzheimer's disease, familial amyotrophic lateral sclerosis, P...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N21/29
CPCG01N21/658G01N21/29
Inventor 李丹郭艳艳
Owner SHANGHAI INST OF TECH
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