Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of taxifolin and kaempferol in combination for preparation of livestock cryopreservative

A cryopreservative, taxifolin technology, applied in application, preservation of human or animal bodies, medical preparations containing active ingredients, etc., can solve problems such as low conception rate, abnormal sperm abundance, sperm inactivation, etc., to achieve Easy to promote, reliable effect, and maintain the effect of fertilization ability

Active Publication Date: 2022-02-18
NORTHWEST A & F UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after freezing and thawing, 40%-50% of the sperm are still inactive, which seriously reduces the utilization rate of the bulls.
However, pig sperm and sheep sperm are more sensitive to freezing stimulation. After thawing, the sperm recovery rate is low, there are many abnormal sperm, and the conception rate is lower than the normal conception rate. Therefore, the freezing of pig sperm and sheep sperm is still in the experimental stage.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of taxifolin and kaempferol in combination for preparation of livestock cryopreservative
  • Application of taxifolin and kaempferol in combination for preparation of livestock cryopreservative
  • Application of taxifolin and kaempferol in combination for preparation of livestock cryopreservative

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation method of pig semen cryoprotectant of the present invention comprises the steps:

[0022] Step 1, prepare the frozen base solution: accurately measure 1.1g of glucose, 1.48g of citric acid, 2.42g of Tris, 0.06g of penicillin sodium, and 0.1g of streptomycin sulfate, dissolve them in 100ml double distilled water, adjust the pH to 6.21, and adjust the osmotic pressure 286mosm / L, prepared as frozen base fluid;

[0023] Step 2, accurately measure 80ml of freezing base liquid, add fresh egg yolk 20ml, taxifolin 0.16mmol, kaempferol 10mg, and mix to obtain a kind of livestock semen cryopreservative (I liquid) of the present invention;

[0024] Get the above-mentioned livestock semen cryoprotectant (I liquid) of 94ml and add 6ml glycerin, filter sterilization, cool to room temperature, can obtain another kind of livestock semen cryopreservation agent (II liquid) of the present invention and be used for preserving pig semen, put Put it in a refrigerator at 4°C ...

Embodiment 2

[0045] The preparation method of goat semen cryopreservation agent of the present invention, comprises the steps:

[0046] Step 1, preparing frozen base liquid, the method is the same as in Example 1;

[0047] Step 2, accurately measure 80ml of frozen base liquid, add 20ml of fresh egg yolk, taxifolin 0.08mmol, and kaempferol 30mg, and mix well to prepare a cryopreservative for livestock semen (I liquid) of the present invention;

[0048] Get the above-mentioned livestock semen cryopreservation agent (I liquid) of 97ml and add 3ml glycerin to be mixed with the mixed solution of 100ml, filter sterilize, cool to room temperature, namely obtain another kind of livestock semen cryopreservation agent (II liquid) of the present invention for use in Save the goat semen and put it in a refrigerator at 4°C for the following experiments.

[0049] Control group 21-23:

[0050] The difference from Example 2 is that taxifolin and kaempferol were not added to control group 21; taxifolin w...

Embodiment 3

[0070] The preparation method of bovine semen cryopreservation agent of the present invention, comprises the steps:

[0071] Step 1, preparing frozen base liquid, the method is the same as in Example 1;

[0072] Step 2: Accurately measure 80ml of frozen base solution, add 20ml of fresh egg yolk, taxifolin 0.14mmol, and 25mg of kaempferol to obtain a livestock semen cryopreservative (I liquid) of the present invention.

[0073] Get 96ml of the above-mentioned livestock semen cryopreservative and add 4ml glycerin to be mixed with 100ml of mixed solution, mix well, filter and sterilize, and cool to room temperature to obtain another livestock semen cryopreservative (II liquid) of the present invention for preservation The bovine semen was placed in a refrigerator at 4°C for the following tests.

[0074] Control group 31-33:

[0075] Different from Example 3, the control group 31 did not add taxifolin and kaempferol; the control group 32 did not add taxifolin; the control group ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of taxifolin and kaempferol for preparing a cryopreservative for livestock. At the same time, a cryopreservative for domestic animals comprising taxifolin, kaempferol and freezing base liquid is disclosed. For the first time in the present invention, taxifolin and kaempferol are used in the cryopreservation of pig, sheep and cattle semen, the effect is reliable and easy to popularize, and it is suitable for long-term storage and long-distance transportation of pigs, sheep and cattle frozen semen in thin tubes, artificial insemination and ultra-low temperature freezing of semen After insemination, it can significantly improve the survival rate, acrosome integrity rate and plasma membrane integrity rate of pig, sheep and cattle sperm after freezing-thawing, and maintain the fertilization ability of sperm.

Description

technical field [0001] The invention belongs to the technical field of ultra-low temperature cryopreservation of mammalian semen, and specifically relates to the application of taxifolin and kaempferol as an antifreeze protective agent in cryopreservation of livestock semen. Background technique [0002] Sperm freezing technology is of great significance. In theory, it can store semen permanently, realize the establishment of semen gene bank, exchange germplasm resources globally, improve the utilization rate of high-quality breeding pigs, and effectively prevent the spread of diseases. The sustainable development of animal husbandry and the sufficient supply of animal products in my country and the world have important practical significance. [0003] Sperm are sensitive to temperature changes. In the process of freezing and cooling, sperm are susceptible to freezing damage, mainly including physical damage, chemical damage and oxidative damage. Compared with fresh semen, t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02A61K31/352A61P39/06
CPCA01N1/0221A01N1/0226A61K31/352A61P39/06
Inventor 李青旺刘定帮杨丽吴琳
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com