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Method applicable to all-female diploid breeding of trouts

A salmon and sports technology, applied in the field of salmon full-female diploid breeding, can solve the problems of non-existence and the like

Inactive Publication Date: 2013-07-31
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problem that there is no all-female diploid breeding method for salmonids, and proposes a method suitable for all-female diploid breeding for salmonids

Method used

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Examples

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specific Embodiment approach 1

[0020] Specific embodiment one: a kind of method that is applicable to salmon all-female diploid breeding in the present embodiment is carried out according to the following steps:

[0021] 1. First, select healthy and mature salmonid females and males to collect roe and semen by artificial means, and place the roe and semen in different beakers;

[0022] 2. After washing fish eggs with normal saline for 1 to 2 times, put the cleaned fish eggs into the spawning tray, and add NaOH with a concentration of 1N; according to the volume ratio of artificial seminal plasma and seminal fluid (80-100) Add artificial seminal plasma to the semen in the beaker at a ratio of :1 to dilute it and put it in a petri dish; apply ultraviolet radiation to the diluted semen, and the irradiation intensity is 3000ergs / mm 2 ~4000ergs / mm 2 , the irradiation time is 50s~70s, wherein, the preparation of physiological saline: 7.5g of NaCl, 0.2g of KCl, 0.2g of CaCl 2 2H 2 O and 0.02 g NaHCO 3 Add to 1...

specific Embodiment approach 2

[0035] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 2, artificial seminal plasma is added to the semen in the beaker for dilution according to the volume ratio of artificial seminal plasma to seminal fluid (85-95): 1 ; Apply ultraviolet radiation to the diluted semen, the radiation intensity is 3200ergs / mm 2 ~3800ergs / mm 2 , The irradiation time is 55s ~ 65s. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0036] Specific embodiment three: the difference between this embodiment and specific embodiment one is that in step 2, according to the volume ratio of artificial seminal plasma and seminal fluid, the ratio of 90:1 is added to the seminal fluid in the beaker to add artificial seminal plasma to dilute; The semen is subjected to ultraviolet radiation with an irradiation intensity of 3500ergs / mm 2 , the irradiation time is 60s. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention relates to a method applicable to all-female diploid breeding of trouts, and is used for solving the problem of currently having no method applicable to all-female diploid breeding of trouts. The method applicable to all-female diploid breeding of trouts comprises the following steps: collecting roes and semen by adopting a manual method; washing roes by normal saline and diluting the semen by using manual seminal plasma; conducting fertilization to roes; incubating all-female diploid; and incubating to obtain young trouts. By adopting the method to conduct all-female diploid breeding of trouts, the all-female rate can achieve 85%; and the method can be applied in the production field of all-female diploid breeding of trouts.

Description

technical field [0001] The present invention relates to a method suitable for all-female diploid breeding of salmonids. Background technique [0002] Since the end of the last century, there have been reports of triploid induction experiments on carps (carp, crucian carp, etc.) , but it is only a small-scale test of laboratory scale, and there is no report of productive test and production so far. [0003] The main purpose of salmon allodiploid is to prepare sex-reversed males, because the preparation of allodiploid is the prerequisite for inducing sex-reversed males, therefore, the preparation method of allodiploid must be solved. Chromosomal manipulations for salmonids are only carried out by a domestic company and some related scientific research institutes in Japan to induce triploid rainbow trout experiments using the hyperthermic shock method. At present, there is no comparable technical example for the method of all-female diploid breeding of salmonids in China. C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐革锋王炳谦谷伟户国
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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