GDF15 rapid quantitative detection device and preparation method thereof

A quantitative detection and rapid technology, applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of insufficient protein adsorption and weak binding force of NC membrane, and achieve the effect of simple structure, simple operation and novel concept.

Active Publication Date: 2020-09-08
JILIN PROVINCE GERUISITE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The invention provides a GDF15 rapid quantitative detection device and its preparation method to solve the problems in the prior art that the amount of protein adsorbed by NC membrane is insufficient and the binding force is not strong

Method used

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  • GDF15 rapid quantitative detection device and preparation method thereof
  • GDF15 rapid quantitative detection device and preparation method thereof
  • GDF15 rapid quantitative detection device and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] see figure 1 , 2 As shown, including sample pad 1, immune colloidal gold glass fiber membrane 2, immune nitrocellulose membrane 3, absorbent pad 4, plastic plate 5, wherein, the sample pad 1, immune colloidal gold glass fiber membrane 2, immune nitrocellulose The plain film 3 and the absorption pad 4 are pasted on the plastic plate 5 respectively, and the two ends of the immune nitrocellulose membrane 3 overlap with the absorption pad 4 and the immune colloidal gold glass fiber membrane 2 respectively, and the immune colloidal gold glass fiber membrane 2 The other end is overlapped with the sample pad 1; a detection line T and a quality control line C are set on the immunonitrocellulose membrane 3; there is a high-specificity growth differentiation factor 15 (GDF15) antibody on the solid phase of the detection line T ; Spray point goat anti-mouse IgG polyclonal antibody on the described quality control line C;

[0039] Preparation:

[0040] (a) Preparation of colloid...

Embodiment 2

[0063] Colloidal gold particles are 40nm;

[0064] The gold-spraying buffer includes: a concentration of 20mM Tris-HCL solution, a sucrose concentration of 12%, a trehalose concentration of 3%, a BSA concentration of 0.7%, and a pH of 8.5;

[0065] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin and polylysine (SIGMA, 150KD), wherein the concentration of polyethylene glycol glycerin is 0.5%, and polylysine The concentration is 0.5%, filtered through a 0.22μm filter membrane, and set aside;

[0066] The concentration of Tris-HCL solution is 0.1M, the concentration of bovine serum albumin BSA is 0.7%, the concentration of casein is 0.15%, and the concentration of surfactant is 0.7%;

[0067] All the other are with embodiment 1.

Embodiment 3

[0069] Colloidal gold particles are 60nm;

[0070] The gold-spraying buffer solution includes: a concentration of 20mM Tris-HCL solution, a sucrose concentration of 20%, a trehalose concentration of 5%, a BSA concentration of 1%, and a pH of 8.5;

[0071] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin, polylysine (SIGMA, 150KD) and PEG2000, wherein the concentration of polyethylene glycol glycerin is 0.5%, poly The concentration of lysine is 0.5%, the concentration of PEG20000 is 0.1%, and it is filtered through a 0.22 μm filter membrane, and it is set aside;

[0072] The concentration of Tris-HCL solution is 0.1M, the concentration of bovine serum albumin BSA is 1%, the concentration of casein is 0.2%, and the concentration of surfactant is 1%;

[0073] All the other are with embodiment 1.

[0074] Further illustrate the effect of the present invention by experiment below.

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Abstract

The invention relates to a GDF15 rapid quantitative detection device and a preparation method thereof, and belongs to the field of medical detection equipment. The device is prepared by adhering a nitrocellulose membrane with a solid phase containing a high-specificity growth differentiation factor 15 antibody and a goat-anti-mouse IgG polyclonal antibody, a glass fiber membrane adsorbed with a colloidal gold labeled growth differentiation factor 15 antibody, a sample pad, absorbent paper and other auxiliary materials. The preparation method comprises the following steps: pretreating a nitrocellulose membrane by adopting a polyethylene glycol glycerol treatment solution; combining a growth differentiation factor 15 antibody firstly with oleic acid modified zinc sulfide nanoparticles and then adsorbing to a nitrocellulose membrane; and preparing the proper gold spraying buffer solution and the sample pad treatment solution. The reaction sensitivity is effectively improved on the basis of ensuring complete release of the immune colloidal gold, and under the same threshold value, the dosage of the immune colloidal gold can be reduced, the cost is saved, and the complexity of production operation is not increased; the detection test paper is high in sensitivity, strong in specificity and strong in practicability.

Description

technical field [0001] The present invention relates to the field of medical testing equipment, in particular to a GDF15 rapid quantitative detection device and a preparation method thereof, which utilize colloidal gold immunochromatography technology and the principle of double-antibody sandwich method to quantitatively detect human GDF15 in whole blood, serum, plasma or related liquid samples The detection device and the preparation method thereof can realize sensitive, specific and rapid detection of GDF15 markers. Background technique [0002] In the field of medicine, accurate early warning and assessment of abnormal conditions in the body have always been one of the main topics for medical workers to study and overcome; for example, what risks will happen to the human body under normal physiological conditions; Early warning, how to accurately assess disease outcome or development, etc.; therefore, it is very necessary to achieve the above goals through an effective ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/558G01N33/58G01N33/543
CPCG01N33/54346G01N33/558G01N33/587G01N33/74G01N2333/495
Inventor 杨小军李欣
Owner JILIN PROVINCE GERUISITE BIOTECHNOLOGY CO LTD
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