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A therapeutic liquid for treating ischemic stroke, its preparation method and application

A technology of ischemic stroke and treatment solution, which is applied in the field of treatment solution for ischemic stroke and its preparation, can solve the problem of limited treatment strategies for cerebral hypoxia, reduce infarct size, improve cell metabolism, Improving the effect of dysfunction

Active Publication Date: 2021-11-09
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current therapeutic strategies to address cerebral hypoxia after stroke are limited

Method used

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  • A therapeutic liquid for treating ischemic stroke, its preparation method and application
  • A therapeutic liquid for treating ischemic stroke, its preparation method and application

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preparation example Construction

[0033] Such as figure 1 and figure 2 As shown, the present invention provides a kind of preparation method of ischemic stroke treatment liquid, comprises the following specific steps:

[0034] S1: culturing Synechococcus elongatus to obtain a resuspension of Synechococcus elongatus;

[0035] S2: modifying up-converting nanoparticles (UCNPs) to obtain a suspension of up-converting nanoparticles;

[0036] S3: Mix the resuspension of Synechococcus elongatus in the step S1 with the resuspension of upconversion nanoparticles in the step S2 in a volume ratio (1-10): 1 to obtain a treatment solution.

[0037] Further, the step S1 specifically includes the following steps:

[0038] S11: Set the concentration to (1-5)×10 6 Synechococcus elongatus culture solution and BG11 medium (blue-green medium) mixed in a volume ratio of 3:(4-6) per μL, followed by a cycle of 8 hours of light and 16 hours of darkness at 25-28°C Continuous training;

[0039] S12: Increase 3-4mL of BG11 medium...

Embodiment 1

[0065] The invention provides a method for preparing an ischemic stroke treatment liquid, comprising the following specific steps:

[0066] S1: set the concentration to 1×10 6 Individual / μL Synechococcus elongatus culture solution and BG11 medium are mixed according to the volume ratio of 3:4, and continuously cultured at 25°C for 8 hours of light and 16 hours of darkness cycle;

[0067] S2: Add 3 mL of BG11 medium every 4 days to offset the amount of evaporation, and continue culturing for 10 days;

[0068] S3: Discard 50% of the culture solution in step S2 when the concentration of the culture solution is supersaturated (i.e., precipitation or bacterial film occurs), and then increase the same volume of BG11 medium to continue culturing for 20 days to obtain a mixed culture solution;

[0069] S4: Take about 7mL of the mixed culture solution, centrifuge at 4000rpm for 10 minutes, discard the supernatant, and then add 200μL of phosphate buffered saline solution with a concent...

Embodiment 2

[0077] The invention provides a method for preparing an ischemic stroke treatment liquid, comprising the following specific steps:

[0078] S1: Set the concentration to 2×10 6 Synechococcus elongatus culture solution and BG11 culture medium of each / mL were mixed according to the volume ratio of 3:5, and were continuously cultured at 26°C for 8 hours of light and 16 hours of dark cycle;

[0079] S2: Add 3 mL of BG11 medium every 5 days to offset the amount of evaporation, and continue to culture for 11 days;

[0080] S3: Discard 50% of the culture solution in step S2 when the concentration of the culture solution is supersaturated (that is, precipitation or bacterial film occurs), and then increase the same volume of BG11 medium to continue culturing for 25 days to obtain a mixed culture solution;

[0081] S4: Take about 9 mL of the mixed culture solution, centrifuge at 5000 rpm for 12 minutes, discard the supernatant, and then add 300 μL of phosphate buffered saline solution ...

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Abstract

The present invention relates to a treatment solution for treating ischemic stroke and its preparation method and application, which is composed of upconverting nano particle resuspension and Synechococcus elongatus resuspension with a volume ratio of 1:(1-10) mixed It also includes a preparation method for ischemic stroke treatment solution, including the following specific steps: S1: cultivating Synechococcus elongatus to obtain a suspension of Synechococcus elongatus; S2: performing upconversion nanoparticles Modification treatment to obtain the upconversion nanoparticle suspension; S3: mix the Synechococcus elongatus suspension in the step S1 with the upconversion nanoparticle suspension in the step S2 in proportion to obtain the treatment liquid. The beneficial effect of the present invention is that it can effectively provide the required oxygen for the human brain, improve the cell metabolism ability of hypoxic brain tissue to a certain extent, not only improve the dysfunction caused by brain hypoxia, but also avoid surgical craniotomy. damage and risks.

Description

technical field [0001] The invention relates to the field of tissue engineering and regenerative medicine, in particular to a treatment liquid for treating ischemic stroke and its preparation method and application. Background technique [0002] Stroke, commonly known as cerebral apoplexy, is an acute cerebrovascular disease. Stroke is currently the second leading cause of death and the leading cause of disability in the world. Globally, the number of deaths caused by stroke is about 6.5 million, and the number of new cases is about 10.3 million. Stroke with high morbidity, high mortality and high disability rate has caused great suffering to individuals and families, and also brought a serious burden to the country and society. Stroke is mainly divided into ischemic stroke and hemorrhagic stroke. Ischemic stroke is local brain tissue caused by the reduction or disappearance of cerebral artery blood supply caused by atherosclerosis, arteriolar disease or cardiac thrombosis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCA61K35/74A61P9/10C12N1/20
Inventor 王琳王征王健夏克尔扎提·肖哈拉提苏强飞
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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