Pomegranate PgBEL1 gene and application thereof

A pomegranate, gene technology, applied in application, genetic engineering, plant genetic improvement and other directions

Inactive Publication Date: 2020-09-04
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the function of BEL1 gene mainly focuses on the model plant Arabidopsis thaliana, and there are few related research reports on horticultural plants

Method used

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  • Pomegranate PgBEL1 gene and application thereof
  • Pomegranate PgBEL1 gene and application thereof
  • Pomegranate PgBEL1 gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0039] This embodiment provides a pomegranate PgBEL1 gene and the encoded BEL1 protein.

[0040] 1. RNA extraction and cDNA synthesis

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Abstract

The invention discloses a pomegranate PgBEL1 gene and an application thereof, and relates to the field of genetic engineering. A nucleotide sequence of the pomegranate PgBEL1 gene is as shown in SEQ ID No.2. Based on pomegranate whole genome data, a full-length coding sequence of the key gene PgBEL1 for regulating the ovule development is cloned, and the expression analysis proves that the pomegranate PgBEL1 gene has great effects on the development of complete and incomplete floral organs in the pomegranate flower development process. The pomegranate PgBEL1 gene is related to the growth and development of ovule in plant pistils, provides a research direction for regulation genes of the development of female and male reproductive organs of plants, also provides a way for the related research on the BEL1 protein function, is applied in the plant organ development gene function research, and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a pomegranate PgBEL1 gene and its application. Background technique [0002] Pomegranate (Punica granatum L.) belongs to the genus Pomegranate of the family Pomegranate. Because it is rich in polyphenols such as punicalagin and anthocyanin, it is known as a "super fruit". An excellent fruit tree with both ornamental and ecological value. There are two types of pomegranate: complete flowers and incomplete flowers. The base of complete flowers has obvious swelling, which is cylindrical in shape and can develop into fruits; the base of incomplete flowers is smaller, bell-shaped in shape, falls off after flowering, and is not fruitful. Abortion of pistils in incomplete flowers of pomegranate is due to abnormal development of ovules and failure to form egg cells. [0003] The plant TALE (Three amino-acid loop extension) gene family encodes a class of transcription ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/02A01H6/00C12Q1/6895C12N15/11
CPCC07K14/415C12N15/827C12Q1/6895C12Q2600/13
Inventor 苑兆和赵玉洁王永伟李变变王沙
Owner NANJING FORESTRY UNIV
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