Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fully humanized anti-hepatitis B virus monoclonal antibody as well as preparation method and application thereof

A monoclonal antibody, fully humanized technology, applied in biochemical equipment and methods, antiviral agents, chemical instruments and methods, etc., can solve the problems of blood-borne disease infection, complex quality control, etc.

Active Publication Date: 2020-09-04
CHANGCHUN NORMAL UNIVERSITY +1
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method of obtaining is very effective, because obtaining good immunoglobulins is particularly dependent on high-titer plasma, the quality control is more complicated, and immunoglobulins are blood products, directly derived from human blood, and have potential blood-derived risk of disease transmission
Therefore, HBIG immunotherapy has certain limitations in clinical application.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fully humanized anti-hepatitis B virus monoclonal antibody as well as preparation method and application thereof
  • Fully humanized anti-hepatitis B virus monoclonal antibody as well as preparation method and application thereof
  • Fully humanized anti-hepatitis B virus monoclonal antibody as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A fully humanized monoclonal antibody against hepatitis B virus, comprising a heavy chain variable region and a light chain variable region; the amino acid sequence of the heavy chain variable region is shown in SEQ ID NO.2; the light chain can be The amino acid sequence of the variable region is shown in SEQ ID NO:4.

[0030] A coding DNA of a fully humanized anti-HBV monoclonal antibody, including a heavy chain variable region and a light chain variable region; the coding DNA sequence of the heavy chain variable region is shown in SEQ ID NO.1; the The coding DNA sequence of the light chain variable region is shown in SEQ ID NO:3.

Embodiment 2

[0032] A preparation method of fully humanized anti-hepatitis B virus monoclonal antibody, comprising the steps of:

[0033] 1. Screening of positive B cells secreting HBV antibody

[0034] 1. Preparation of EBV virus

[0035] B95.8 cells were cultured in RPMI1640 medium with 10% FBS; after 96 hours, the cell culture suspension was collected and centrifuged at 400g for 10 minutes at 4°C. Harvest the supernatant, filter the supernatant with a 0.45-micron filter membrane, and obtain the EBV virus; aliquot the EBV virus and store it in a -80°C refrigerator.

[0036] 2. Isolation of peripheral blood lymphocytes

[0037] Select healthy people who have been injected with hepatitis B vaccine in the past three to six months and are negative for hepatitis B surface antigen, e antigen, and positive for hepatitis B surface antibody as B cell donors; draw 30 ml of peripheral blood from the donor, and use lymphocyte separation fluid (Histopaque -1077) were isolated from peripheral blood...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fully humanized anti-hepatitis B virus monoclonal antibody as well as a preparation method and an application thereof, and belongs to the technical field of biomedicine and genetic engineering antibody. The monoclonal antibody comprises a heavy chain variable region and a light chain variable region; the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO. 2; the amino acid sequence of the light chain variable region is shown as SEQ ID NO: 4; the coding DNA of the antibody comprises a heavy chain variable region and a light chain variable region, wherein the coding DNA sequence of the heavy chain variable region is shown as SEQ ID NO. 1; and the coding DNA sequence of the light chain variable region is shown as SEQ ID NO: 3. According to the method, B cells are immortalized through EBV virus, the natural fully-humanized hepatitis B virus monoclonal antibody is screened by combining a limited dilution method, and the screening method does not need a large expensive instrument, is simple and easy to implement and is suitable for being carried out in a conventional biology laboratory.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and genetic engineering antibodies, and specifically relates to a fully humanized anti-hepatitis B virus monoclonal antibody, a preparation method and an application thereof. Background technique [0002] According to statistics, about 2 billion people in the world are infected with hepatitis B virus (HBV), about 300-500 million people suffer from HBV chronic infection, and 1-1.5 million patients worldwide die each year from acute or chronic HBV infection. Cirrhosis, liver failure and liver cancer. 25%-40% of them eventually die from cirrhosis and liver cancer (HCC). The World Health Organization (WHO) reports that hepatitis B ranks seventh among the top 10 causes of death worldwide. [0003] Hepatitis B treatment can be divided into two categories: one is antiviral therapy and the other is immunotherapy. Antiviral drugs mainly include alpha interferon (IFNα) and nucleoside drugs, such as c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C12N15/13A61K39/42A61P31/20A61P1/16G01N33/577G01N33/569
CPCC07K16/082A61P31/20A61P1/16G01N33/577G01N33/56983C07K2317/56C07K2317/76G01N2333/02G01N2469/10Y02A50/30
Inventor 宋相伟王雪丽张丽辉辛树权
Owner CHANGCHUN NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com