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Application of Bacillus subtilis mes810 in the control of white silk root rot of grape

A technology of Bacillus subtilis and MES810, applied in application, bacteria, fungicides and other directions, can solve the problems of non-compliance with the development direction of green prevention and control and ecological agriculture, the drug effect is not long-lasting, and the operation is cumbersome, so as to prevent grape white silk disease. , easy to use in a large area, easy to drip irrigation effect

Active Publication Date: 2021-09-07
天津开发区坤禾生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method is cumbersome to operate, and the chemical agent has quick effect, but the effect is not long-lasting, and it will pollute the environment, which is not in line with the development direction of green prevention and control and ecological agriculture
[0004] The use of microorganisms to control plant soil-borne diseases is one of the most effective control measures. Microbial control of plant diseases has the advantages of safety, effectiveness, and no environmental pollution, which is in line with the development direction of future agricultural green prevention and control. After searching, no use of subtilis spores has been found so far. Report on Control of Grape Blight with B.subtilis

Method used

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  • Application of Bacillus subtilis mes810 in the control of white silk root rot of grape
  • Application of Bacillus subtilis mes810 in the control of white silk root rot of grape
  • Application of Bacillus subtilis mes810 in the control of white silk root rot of grape

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation of embodiment 1 bacillus subtilis MES810 microbial bacterial agent

[0045] The preparation steps of Bacillus subtilis MES810 microbial inoculum are as follows:

[0046] (1) Shake flask culture: carry out seed shake flask culture through the strain activated on the slant, the shake flask culture medium is beef extract peptone medium, the formula of beef extract peptone medium is: beef extract 0.5%-3%, peptone 0.5% -3%, sodium chloride 0.2-1%, medium pH 6.0-7.5. The culture conditions are: rotation speed 200-230r / min, temperature 32-37°C, culture for 10-12h;

[0047] (2) Primary seed culture: 100mL primary seed culture medium is placed in a 500mL eggplant bottle, sterilized by moist heat at 116°C-121°C for 20-35min, and 10mL live Bacillus subtilis freeze-dried tube strains are inoculated in the primary stage In the seed medium, cultivate at 30°C-37°C for 10-12h; the raw materials and dosage of the medium used for primary seed cultivation are: beef extra...

Embodiment 2

[0054] Example 2 Antagonism Test of Bacillus subtilis MES810 to Grape Blight

[0055] (1) Test time and place: In February 2019, it will be conducted in the laboratory of the company's R&D center.

[0056] (2) Test method:

[0057] (1) The source of the test grape sclerotium pathogen: Grape sclerotium was collected from the grape sclerotium strain in Wuqing District, Tianjin. The company's researchers isolated and purified the strain, and the strain was identified as Sclerotium rolfsii Sacc by 16SrDNA. ). The determination of pathogenicity showed weak pathogenicity.

[0058] (2) Tablet confrontation experiment:

[0059] First place the grape pathogen in the center of the LB plate, and then place the activated Bacillus subtilis MES810 at a place 2.0 cm away from the indicator bacteria, and set up a blank control. Cultivate at a constant temperature of 37°C. When the blank control is about to cover the entire petri dish, measure the control growth (colony radius) and treatme...

Embodiment 3

[0063] Example 3 Control Effect Test of Bacillus subtilis MES810 on Grape Blight

[0064] (1) Test drug

[0065] (1) Microbial agent: the MES810 liquid preparation prepared in Example 1 was diluted with water 300 times, 500 times and 800 times respectively;

[0066] (2) Chemical agent: 50% dysonium (Jinan Yongtai Chemical Industry), diluted 500 times with water.

[0067] (3) Blank control: clear water.

[0068] (2) Test time and place: in April 2019 in Wuqing Grape Planting Base;

[0069] (3) Test method: before the test, investigate and count the number of sick plants and the disease index of each diseased strain of the diseased grape silkworm, and dig a 15 cm wide and 20 cm deep annular ditch at a distance of 20 centimeters from the root of each plant. 5 plants, repeated three times. Strains were selected by random block arrangement method. Take 3L of the MES810 liquid preparation diluted 300 times, 500 times and 800 times of Example 1 and pour them evenly into the circ...

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Abstract

The invention belongs to the technical field of using microorganisms to prevent and control soil-borne plant diseases, and particularly relates to the application of Bacillus subtilis MES810 in the prevention and treatment of white silk root rot of grapes. It was deposited in the China General Microorganism Culture Collection Management Center on April 10, and the preservation number is CGMCC No.14514. The invention also provides the application of the subtilis bacillus MES810 to the grape white silk root rot. The beneficial effects of the present invention are: it has obvious inhibitory effect on the pathogenic bacteria of grape silkworm root rot, and the fungal agent of the present invention is used to prevent and treat grape silkworm, the control effect is remarkable, and it is environmentally friendly, low-cost, and convenient for large-scale production. area used.

Description

technical field [0001] The invention belongs to the technical field of using microorganisms to prevent and control soil-borne plant diseases, and in particular relates to the application of a bacillus subtilis MES810 in preventing and controlling grape white silk root rot. Background technique [0002] Grape root rot is a serious disease of the whole plant, which will cause the death of the whole plant and seriously hinder the development of the grape industry. Many diseases can cause grape root rot, mainly including white-striped feather disease, purple-striped feather disease, silkworm, root rot and round-spotted root rot. Among them, grape scurvy is a disease caused by the half-knowledge fungus Sclerotium rolfsii Sacc. Most of the pathogenic bacteria are weak parasites. It takes one to several years from a small amount of root disease to main root rot until the aboveground part of the plant shows symptoms. Grape silkworm mainly occurs on the roots and necks of seedlings...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/22A01P3/00C12N1/20C12R1/125
CPCA01N63/22C12N1/20
Inventor 顾欣燕肖培英刘金龙王秀丽刘刚鲁玉峰
Owner 天津开发区坤禾生物技术有限公司
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