Method for detecting leukocyte mitochondrial respiratory function and method for detecting kidney yin deficiency symptom

Abstract

The invention discloses a method for detecting leukocyte mitochondrial respiratory function and a method for detecting kidney yin deficiency symptom. The method for detecting leukocyte mitochondrial respiratory function can comprise the steps of S1 extracting leukocytes: collecting and separating a blood sample, and extracting the leukocytes in the blood sample; (S2) inoculating the leukocytes: treating a cell culture plate by using a cell adhesive, preparing a leukocyte culture medium, diluting the leukocytes by using the leukocyte culture medium, and inoculating the leukocytes onto the cellculture plate; and (S3) detecting the mitochondrial respiratory function: monitoring the mitochondrial respiratory function in the leukocytes in real time by detecting the oxygen consumption rates ofthe leukocytes inoculated in the cell culture plate in different states. According to the method, the mitochondrial function in a complete cell state can be detected, and the cell energy metabolism process can be monitored in real time.

Description

technical field [0001] The invention generally relates to the field of biotechnology, and more specifically, relates to a method for detecting the respiratory function of leukocyte mitochondria and a method for detecting experimental animals with kidney yin deficiency by using the method. Background technique [0002] Mitochondria are important places for energy production in the body, and more than 90% of ATP in cells is provided by mitochondria. Studies have shown that the occurrence of various human chronic diseases (such as tumors, cardiovascular diseases, and neurodegenerative diseases) is closely related to abnormal cellular energy metabolism. Therefore, the study of mitochondrial function and the real-time monitoring of cellular energy metabolism have important guiding significance for clinical disease diagnosis and drug development. At present, the traditional research method of mitochondrial function is to extract mitochondrial components after crushing cells, and ...

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Problems solved by technology

At present, the traditional research method of mitochondrial function is to extract mitochondrial components after crushing cells, and then carry out corresponding physiological and biochemical index detection, which has many shortcomings such as impurity pollution, decreased mitochondrial activity, etc., and cannot completely objectively reflect the energy metabolism process of cells

[0003] Kidney yin deficiency is one of the important symptoms in the theoretical system of traditional Chinese medicine. Abnormal material metabolism and energy metabolism of the body are the typical characteristics of this symptom. However, the subjective uncertainty of the "symptom" in traditional Chinese medicine makes its clinical evaluation indicators controversial. Now There is still a lack of technical methods suitable for this study

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