Application of trepang zymohydrolysis element to skin whitening products
A technology of enzymes and sea cucumbers, applied in cosmetics, cosmetic preparations, cosmetic preparations, etc., can solve problems such as excessive molecular weight, waste of resources, skin damage, etc., and achieves simple preparation process, mild reaction conditions, important economical Effects of Value and Social Benefit
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Embodiment 1
[0070] Experimental materials: Tyrosinase and L-dopa were purchased from Shanghai Baoman Biotechnology Co., Ltd.; Na 2 HPO 4 , NaH 2 PO 4 , HCL was purchased from Jinan Dingguo Biotechnology Co., Ltd.; samples 1 and 2 were produced by Rongcheng Huihai Chuangda Biotechnology Co., Ltd.
[0071] Sample preparation:
[0072] (1) Sample 1: Sea Cucumber Enzyme (trade name, specifically prepared according to the preparation method of Example 1 of CN 109251954 A)
[0073] (2) Sample 2: sea cucumber peptide solution (sample obtained after purification of sample 1, only retaining sea cucumber peptide components)
[0074] Solution preparation:
[0075] (1) PBS buffer: weigh 15.6gNa 2 HPO 4 and 35.8gNaH 2 PO 4 , respectively dilute to 500ml with deionized water, take 255mlNa 2 HPO 4 and 245mlNaH 2 PO 4 The solution was mixed and stored at 4°C for later use.
[0076] (2) L-dopa solution: weigh 0.0493g of L-dopa, dissolve in a small amount of dilute hydrochloric acid, add PBS ...
Embodiment 2
[0109] A sea cucumber facial mask, characterized in that the facial mask is made of non-woven facial mask paper and facial mask multifunctional liquid, and the facial mask multifunctional liquid comprises the following components according to 100 parts by weight, sea cucumber enzyme enzyme 0.1% -0.5%, base fluid 99.9% -99.5%.
[0110] Product of the present invention carries out following test:
[0111] Add 0.1%-0.5% sea cucumber fermentin into the base solution, stir to dissolve evenly, and seal the mask bag.
[0112] Select 100 test subjects aged 25-55 with rough skin, enlarged pores, dull skin, and sallow complexion. The age composition is as follows: 38 people aged 25-35, 35 people aged 36-45, and 27 people aged 46-55. After that, apply this mask every day in the first week, and apply the mask every other day from the second week to the fourth week. The total application cycle is 28 days, and other masks are not allowed during this period.
[0113] After 28 days of using...
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