A strain of Lactobacillus casei that can alleviate osteoporosis and its application
A technology of Lactobacillus casei and osteoporosis, applied in the field of microorganisms, can solve the problems of increasing the risk of venous thromboembolism and stroke, and achieve the effect of increasing the thickness of trabecular bone and high safety
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Embodiment 1
[0076] Embodiment 1: the acquisition of Lactobacillus casei
[0077] Specific steps are as follows:
[0078] Taking fresh feces from healthy people in Ningde, Fujian as samples, draw 0.5mL sample and add it to 5mL MRS liquid medium, culture at 37°C for 18-24h, and enrich to obtain the enriched sample; draw 0.5mL enriched The pooled sample was added to 4.5mL sterile saline to obtain 10 -1 Diluent, then draw 0.5mL 10 -1 Dilution in 4.5mL of normal saline to obtain 10 -2 Diluent, according to this operation, successively get 10 -3 , 10 -4 , 10 -5 , 10 -6 Dilution; draw 100 μL of gradient dilution and spread it on the MRS solid medium, 10 -4 , 10 -5 , 10 -6 1 plate for each gradient, cultivated at 37°C for 48 hours to obtain colonies; select the colonies with typical characteristics of Lactobacillus casei on the MRS solid medium according to the shape, size, edge, and transparency of the colonies, and use an inoculation loop to pick the colonies on the MRS solid culture ...
Embodiment 2
[0084] Example 2: Effects of Lactobacillus casei on bone mineral density, bone volume fraction and trabecular bone thickness in ovariectomized rats
[0085] Specific steps are as follows:
[0086] Take 20 SPF grade female SD rats with a body weight of 250±20g, and divide them into 4 groups at random, 5 rats in each group. CCFM1127 group and HN13 group gavaged with Lactobacillus casei HN13 suspension.
[0087] The experiment lasted for 9 weeks: the first week was the adaptation period for the rats. During the adaptation period, the rats were fed in an environment with a temperature of 22±2°C, a humidity of 40-70%, and 12 hours of alternating day and night. The feed used was purchased from Suzhou Shuangshi Rat breeding compound feed from Experimental Animal Feed Technology Co., Ltd. was used for the experiment after a week of normal diet adaptation.
[0088] After the end of the first week, the rats in the model control group, CCFM1127 group and HN13 group were anesthetized by...
Embodiment 3
[0093] Embodiment 3: Effect of Lactobacillus casei on immune factor levels in ovariectomized rat serum
[0094] Specific steps are as follows:
[0095] Take 20 SPF grade female SD rats with a body weight of 250±20g, and divide them into 4 groups at random, 5 rats in each group. CCFM1127 group and HN13 group gavaged with Lactobacillus casei HN13 suspension.
[0096] The experiment lasted for 9 weeks: the first week was the adaptation period for the rats. During the adaptation period, the rats were fed in an environment with a temperature of 22±2°C, a humidity of 40-70%, and a 12-hour alternation of day and night. The feed used was purchased from Suzhou Shuangshi Rat breeding compound feed from Experimental Animal Feed Technology Co., Ltd. was used for the experiment after a week of normal diet adaptation.
[0097] After the end of the first week, the rats in the model control group, CCFM1127 group and HN13 group were anesthetized by intraperitoneal injection of 10% (v / v) chlo...
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