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Method for identifying labiatae medicinal plants by using salvia miltiorrhiza EST-SSR markers

A technology of medicinal plants and Labiatae, applied in the field of molecular biology, can solve the problem of few EST sequences

Active Publication Date: 2020-08-18
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many medicinal plants belonging to the same family as Salvia miltiorrhiza, and there are many bulk medicinal materials such as perilla, baicalin, mint, etc., but compared with Salvia miltiorrhiza, the EST sequences that can be used for these medicinal plants are relatively less

Method used

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  • Method for identifying labiatae medicinal plants by using salvia miltiorrhiza EST-SSR markers
  • Method for identifying labiatae medicinal plants by using salvia miltiorrhiza EST-SSR markers
  • Method for identifying labiatae medicinal plants by using salvia miltiorrhiza EST-SSR markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Universal Research Test of Danshen EST-SSR Marker

[0023] No.1 Materials and methods

[0024] No.1.1 Test material and sampling

[0025] The materials used in this experiment include: Salvia miltiorrhiza, a medicinal plant of the family Labiatae, Salvia miltiorrhiza S. miltiorrhiza Bunge, White Flower Salvia Salvia miltiorrhiza Bge.f.alba, Gansey sage S. przewalskii Maxim., Yunnan sage, S. yunnanensis C.H.Wright; Xinjiang sage, S. deserta Schang.; a bunch of red, S. splendens var.Nana; perilla, Perilla frutescens (L.) Britt.; Skullcap, Scutellaria baicalensis Georg.; Mint, Mentha haplocalyx Briq. Motherwort, Leonurus Artemisia (Lour.) S.Y., and the medicinal plant Campanulaceae (Lour.) Platycodon grandiflorum (Jacp.) A.DC) a total of 11 kinds of medicinal plants. The materials were planted in the Medicinal Botanical Garden of Shandong Agricultural University and identified by Professor Wang Jianhua. Take an appropriate amount of...

Embodiment 2

[0048] Example 2 Identification of 10 Labiatae Medicinal Plants Using Danshen EST-SSR Markers

[0049] Among the 48 pairs of EST-SSR markers screened in Example 1, 2 pairs of Danshen EST-SSR markers with relatively large differences were selected to carry out the identification test of 10 kinds of medicinal plants of Labiatae.

[0050] No.1 Materials and methods

[0051] No.1.1 Material

[0052] The 11 kinds of medicinal plant materials were all collected from the Medicinal Botanical Garden of Shandong Agricultural University, and their families and genera and related information are shown in Table 3.

[0053] Table 3 11 test materials of medicinal plants and their families and genera

[0054]

[0055] No.1.2 method

[0056] No.1.2.1 Extraction of DNA

[0057] Take an appropriate amount of young leaves (about one to two pieces) from each material, freeze them quickly in liquid nitrogen, extract DNA using the TIANGEN plant genomic DNA kit rapid extraction method, and sto...

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Abstract

Due to the lack of appropriate molecular markers, the recognition research of most of labiatae medicinal plants is almost blank. An EST-SSR primer developed from salvia miltiorrhiza bunge is utilized,salvia alba bunge, salvia officinalis, salvia officinalis Yunnan, salvia officinalis Xinjiang, salvia splendens, perilla frutescens, scutellaria baicalensis, mint, motherwort and platycodon grandiflorum are taken as materials, the universality of the salvia miltiorrhiza bunge EST-SSR primer among labiatae is detected, and clustering analysis is carried out. Universal detection results show that the radix salviae miltiorrhizae primers effectively amplify all test materials, and new EST-SSR markers and recognition references can be provided for research of labiatae medicinal plants.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a method for identifying medicinal plants of Labiatae by using salvia miltiorrhiza EST-SSR markers. Background technique [0002] Due to the lack of suitable molecular markers, the research on the identification of most medicinal plants of Labiatae is almost blank. [0003] Expressed Sequence Tags (Expressed Sequence Tags EST) are the 5' or 3' end sequences obtained by large-scale sequencing of randomly selected clones from the cDNA library, and the length is generally 150-500bp. The Simple Sequence Repeat (SSR) marker established based on expressed sequence tags is called EST-SSR. Compared with the simple repeat sequence markers derived from traditional genomes, this new type of SSR marker is gradually showing its unique advantages. As a part of a gene, EST-SSR flanking sequences are highly conserved among species, can provide "absolute" markers for functional genes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 宋振巧王建华宗成堃冯园园林彩彩
Owner SHANDONG AGRICULTURAL UNIVERSITY
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