An engineering strain of Serratia marcescens and its application in the production of prodigiosin

Abstract

The invention discloses a Serratia marcescens engineering bacterium and its application in the production of prodigiosin, belonging to the field of biotechnology. The invention provides a Serratia marcescens engineering bacterium JNB5‑1ΔcpxR capable of high prodigiosin production, and the Serratia marcescens engineering bacterium JNB5‑1ΔcpxR is obtained by knocking out Serratia marcescens JNB5‑1 Inoculated with the gene encoding the response regulator protein CpxR, the Serratia marcescens engineered bacterium JNB5-1ΔcpxR was inoculated into the fermentation medium and fermented for 96 hours, and the prodigiosin production in the fermentation broth could be as high as 5.83g / L , 41.9% higher than that of wild-type Serratia marcescens JNB5‑1.

Description

technical field [0001] The invention relates to a Serratia marcescens engineering bacterium and its application in producing prodigiosin, and belongs to the field of biotechnology. Background technique [0002] Prodigiosin is a kind of natural red pigment containing a tripyrrole skeleton structure, which is mainly produced by some microorganisms and has various biological activities such as anti-cancer, immunosuppression, and anti-insect. extensive attention of researchers. [0003] At present, the methods for producing prodigiosin mainly include chemical synthesis method and microbial fermentation method. Among them, the chemical synthesis method mainly obtains prodigiosin through tandem conjugate addition and high temperature dehydrogenation. However, due to the complex and difficult pathways and low yields, chemical synthesis is difficult to achieve large-scale industrial production. The principle of microbial fermentation is to obtain prodigiosin through microbial fer...

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Problems solved by technology

[0004] However, there are still certain defects in the existing biological methods, among which the low yield is the most important defect hindering the industrialization of microbial fermentation methods

For example, Kim et al. produced prodigiosin by inoculating Hahella chejuensis KCTC 2396 into the 2216 medium of marine bacteria broth for fermentation. However, using this method to ferment for 40 hours, only the concentration of prodigiosin in the fermentation broth Yield up to 28mg / L (specific references: Kim S J, Lee H K, Lee YK, et al. Mutant selection of Hahella chejuensis KCTC 2396 and statistical optimization of medium components for prodigiosin yield-up [J]. ):183-188.); Martha Ingrid Gutiérrez-Román et al fermented Serratia marcescens CFFSUR-B4 by inoculating it into a peanut-based medium to produce prodigiosin, however, using this method for 24h of fermentation, only the fermentation broth The production of prodigiosin and chitinases by tropical Serratia marcescens strains with potential to control plant pathogens [J].World Journal of Microbiology and Biotechnology,2012,28(1):p.145-153.)

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