A kind of mixed stem cell injection containing three kinds of angiogenesis properties and its preparation method

Abstract

The invention relates to a mixed stem cell injection containing three vascular regeneration attributes. The mixed stem cell injection comprises Wharton jelly mesenchymal stem cells (WJMSCs), human umbilical artery adventitia periodontal cells (APCs) and human umbilical artery adventitia Flk-1 + cells. Umbilical cords abandoned by newborns are used as a material source; the method comprises the following steps: extracting Flk-1 + cells and umbilical artery peripheral cells APC from umbilical artery adventitia; the two cells provide two direct and specific precursor cells for vascular regeneration, and through the extracellular matrix components of the new blood vessel supplemented, updated and formed by the WJMSCs, a regeneration microenvironment for differentiation of the vascular precursor cells into the blood vessel under regulation and control of a vascular regeneration signal system is further formed. The three stem cells are mixed in proportion for an in-vitro culture experiment,and the result proves that the vascular net is successfully formed after in-vitro culture is performed for 13 days. The injection provided by the invention can be prepared into an injection for vascular regeneration, and is used for treating ischemic heart disease, ischemic stroke, ischemic peripheral vascular disease and other diseases.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an injection containing stem cells with various vascular regeneration properties and a preparation method thereof. Background technique [0002] Ischemic diseases caused by vascular lesions, such as coronary heart disease, cerebral infarction, ischemic necrosis of limbs, and even eye ischemic blindness, etc., have been the top causes of death and disability in the world so far. Modern medicine has failed to fundamentally improve its prognosis with drugs, intervention, surgery, and organ transplantation. Although stem cell regenerative medicine has brought new opportunities in the 21st century, the specificity, functionality, and safety of seed cell regeneration of blood vessels have greatly restricted the realization of the concept of stem cell regeneration of blood vessels. [0003] At present, although embryonic stem cells (ESC) and induced pluripotent stem cells ...

AI Technical Summary

Problems solved by technology

Modern medicine has failed to fundamentally improve its prognosis with drugs, intervention, surgery, and organ transplantation

Although stem cell regenerative medicine has brought new opportunities in the 21st century, the specificity, functionality, and safety of seed cell regeneration of blood vessels have greatly restricted the realization of the concept of stem cell regeneration of blood vessels

However, it is clear that it is very difficult and invasive to obtain material from the adventitia of the patient's blood vessels, and it is difficult to promote

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