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Asexual propagation method of merremia boisiana

A technology of vine and seeds is applied in the field of plant reproduction and cultivation, which can solve the problems of low survival rate, low germination rate of seed seedlings, separation of progeny traits, etc., so as to improve the survival rate, reduce the chance of mildew, and save materials.

Active Publication Date: 2020-08-18
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for asexual propagation of Admirallum vine, which solves the problems of low survival rate of traditional cutting system, low germination rate of seed seedlings and separation of offspring production characters. A large number of genotype individuals rapidly asexually reproduce, so that the genotype individuals will produce a large number of isogenic plants after a certain period of time

Method used

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  • Asexual propagation method of merremia boisiana
  • Asexual propagation method of merremia boisiana
  • Asexual propagation method of merremia boisiana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The method for asexual propagation of Admirallum vine of the present invention comprises the following steps:

[0023] (1) Seed pretreatment: put the seeds of Admirallum vine into a petri dish, disinfect with 5% sodium hypochlorite solution of available chlorine for 20 minutes, move the petri dish into a super-clean bench after disinfection, and use 75% alcohol to clean it for 15 minutes in the super-clean bench. seconds, and then use sterile water to wash twice, on the filter paper, use tweezers and scissors to cut the seed embryos of the tweezers and scissors at the tip of the seeds. A small square mouth with a side length of 1 mm; wherein the tweezers and scissors are soaked in a 75% alcohol container before operation , Rinse off the alcohol on the surface of the vessel with sterile water before each operation, and continue to soak in 75% alcohol after operation to reduce the possibility of contamination;

[0024] (2) Seed culture: put the sheared seeds into a pre-hi...

Embodiment 2

[0028] The method for asexual propagation of Admirallum vine of the present invention comprises the following steps:

[0029] (1) Seed pretreatment: Put the seeds of Admirallum vine into a petri dish, disinfect with 0.1% mercuric chloride solution for 15 minutes, move the petri dish into an ultra-clean bench after disinfection, and use 75% alcohol to clean it for 15 seconds in the ultra-clean bench , and then use sterile water to wash twice, cut the seed embryo of Admirallum vine on the filter paper with tweezers and scissors to cut a small square mouth with a side length of about 2 mm at the tip of the seed, and soak it in a 75% alcohol container before the tweezers and scissors are operated. Rinse off the alcohol on the surface of the vessel with sterile water before each operation, and continue to soak in 75% alcohol after operation to reduce the possibility of contamination;

[0030] (2) Seed culture: put the sheared seeds into a pre-high temperature steam sterilized tissu...

Embodiment 3

[0034] The method for asexual propagation of Admirallum vine of the present invention comprises the following steps:

[0035] (1) Seed pretreatment: put the seeds of Admirallum vine into a petri dish, disinfect with 5% sodium hypochlorite solution of available chlorine for 20 minutes, move the petri dish into a super-clean bench after disinfection, and use 75% alcohol to clean it for 15 minutes in the super-clean bench. Second, use sterile water to wash twice again, on the filter paper, cut the seed embryo of Admirallum vine with tweezers and scissors at the tip of the seed to cut a side length of 1.5 mm, and soak it in a 75% alcohol container before the tweezers and scissors are operated. Rinse off the alcohol on the surface of the instrument with sterile water before each operation, and continue to soak in 75% alcohol after operation to reduce the chance of contamination;

[0036] (2) Seed culture: put the sheared seeds into a pre-high temperature steam sterilized tissue cul...

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Abstract

The invention discloses an asexual propagation method of merremia boisiana. The asexual propagation method comprises the following steps of (1) seed pretreatment; (2) seed culture; (3) expanding propagation of aseptic seedlings; and (4) expanding propagation of new plants. The method solves the problems that a traditional cuttage system is low in survival rate and germination rate of seed seedlings and character separation is generated in offspring, can enable any one merremia boisiana genotype individual with excellent characters to be massively, rapidly and asexually propagated, and enablesthe genotype individual to generate a large number of plants with the same genotype after a certain period of time.

Description

technical field [0001] The invention belongs to the technical field of plant propagation and cultivation, and relates to a technique for cultivating clones of Admirallum vine seeds, in particular to a method for vegetative propagation of Admirallum vine. Background technique [0002] Admiralty vine, also known as Duohuashanzhucai, is a perennial woody vine of the Convolvulaceae Jasmine vine. Its leaves can be used as food for pigs or other livestock, and have the value of being used as feed. Admiralty vine grows in the edge of secondary forest, and its growth range gradually increases with the increase of temperature in recent years, and there is a trend of disaster change. Existing studies have made great progress in proving the mechanism of the rapid growth of Admiralty vine. The photosynthetic rate of Admiralty vine is sensitive and strong to the positive response of light and heat. The photosynthetic rate and growth rate increased significantly, and the seeds of Admiral...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/08A01C1/00
CPCA01H4/008A01C1/08A01C1/00
Inventor 张守信周韬龙文兴
Owner HAINAN UNIVERSITY
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