Specific molecular markers for aspergillus flavus infection mildew of corns, peanuts and walnuts and method for carrying out early mildew detection by utilizing specific molecular markers

Abstract

The invention discloses specific molecular markers for aspergillus flavus infection mildew of corns, peanuts and walnuts. The specific molecular markers for aspergillus flavus infection mildew of corns comprise ethyl acetate-D, and 3-hydroxybutane-2-ketone-D; the specific molecular markers for aspergillus flavus infection mildew of peanuts comprise caproic acid, 2.3-butanedione, 2-hexyl-1-alcohol-M, pentane-1-alcohol-M and hexaldehyde; and specific molecular markers for aspergillus flavus infection mildew of the walnuts comprise ethyl acetate-D, and 3-methyl-2-butyl alcohol. The method for carrying out early-stage mildew detection by utilizing the specific molecular markers for aspergillus flavus infection mildew of the corns, the peanuts and the walnuts comprises the following steps: establishing a complete GC-IMS database of the corn, the peanut and the walnut, and analyzing to obtain the specific molecular markers for aspergillus flavus infection mildew of the corn, the peanut and the walnut; and extracting corn, peanut and walnut stock samples, carrying out GC-IMS technical detection to obtain GC-IMS data of the stock samples, analyzing whether the specific molecular markers for aspergillus flavus infection mildew exist in the GC-IMS data, and judging whether the stock samples have early mildew or not.

Description

technical field [0001] The invention relates to the technical field of crop storage detection, in particular to a specific molecular marker for corn, peanut, walnut, and mildew infected by Aspergillus flavus and a method for early detection of mildew using the marker. Background technique [0002] Corn is one of the cereal plants with the highest yield in the world, ranking third in the staple food after wheat and rice; peanut is a highly important crop used for oil and protein production in China; walnut is widely planted in China and has a huge output ; but the above three crops are highly sensitive to the infection of Aspergillus flavus in the production process, and they are all faced with the problem of aflatoxin pollution caused by the infection and mildew of Aspergillus flavus in the storage process; aflatoxin has serious harm to the human body Toxicological impact, eating corn, peanuts, and walnuts contaminated by aflatoxin can cause serious health damage to the eate...

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Problems solved by technology

[0002] Corn is one of the cereal plants with the highest yield in the world, ranking third in the staple food after wheat and rice; peanut is a highly important crop used for oil and protein production in China; walnut is widely planted in China and has a huge output ; but the above three crops are highly sensitive to the infection of Aspergillus flavus in the production process, and they are all faced with the problem of aflatoxin pollution caused by the infection and mildew of Aspergillus flavus in the storage process; aflatoxin has serious harm to the human body Toxicological impact, eating corn, peanuts, and walnuts contaminated by aflatoxin can cause serious health damage to the eaters; In the late stage, it loses its edible value, thus causing huge economic losses; but if it is detected in time and properly treated in the early stage of Aspergillus flavus infection and mildew, corn, peanuts, and walnuts still have edible value and will not have health effects on the human body. Therefore, the economic loss caused by mildew of corn, peanuts, and walnuts can be greatly reduced; but at present, there is a lack of simple, rapid, and effective technical means for early detection of mildew by Aspergillus flavus in the storage process of corn, peanuts, and walnuts

[0003] In recent years, GC-IMS gas phase-ion mobility spectrometry technology has developed rapidly and has been widely used in food quality and safety testing; at present, there are also studies on the application of GC-IMS in the early detection of mildew in the grain storage process. The change rule of all volatile organic components in the early stage of grain mildew in storage, and in the middle and late stages of mildew is used to detect the early mildew of grain; but there is a problem in this method: the same grain crop has different Varieties, the changing rules of all volatile organic components in the initial stage of storage and during the mildew process are not the same, so this method is not universal; The research on the change rule of all volatile organic components in the late process can be used for the detection of early mildew in japonica rice storage, but it is ineffective for glutinous rice. Therefore, different detection systems need to be established for different varieties of rice; and there are many kinds of crops. There are many varieties of each crop, so it is obviously not practical to use this method

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