A strain of Lactobacillus crispatus capable of preventing and/or treating candida vaginitis

A technology of Lactobacillus crispatus and Candida, which is applied in the field of microorganisms, can solve the problems of poor curative effect of Candida vaginitis, and achieve the effect of relieving vaginal inflammation, reducing concentration and reducing quantity

Active Publication Date: 2022-02-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, antibiotic therapy can lead to the emergence of antibiotic-resistant strains, making antibiotics less and less effective in treating candidal vaginitis

Method used

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  • A strain of Lactobacillus crispatus capable of preventing and/or treating candida vaginitis
  • A strain of Lactobacillus crispatus capable of preventing and/or treating candida vaginitis
  • A strain of Lactobacillus crispatus capable of preventing and/or treating candida vaginitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Screening and strain identification of Lactobacillus crispatus

[0057] 1. Screening

[0058] Chicken feces, human feces, and female vaginal swabs from Anhui, Sichuan, and Jiangsu were used as samples. After pretreatment, the samples were stored in 30% glycerol in a -80°C refrigerator. After taking out and thawing, mix the samples Draw 0.5mL sample and add it to 4.5mL, and perform gradient dilution with 0.9% physiological saline, select the appropriate gradient dilution solution and spread it on the MRS solid medium, culture it at 37°C for 48h, and pick the typical colonies of Lactobacillus crispatus to the MRS solid medium Streak and purify the medium, pick a single colony and transfer it to the MRS liquid medium for enrichment, and preserve it in 30% glycerol to obtain strains CCFM1110, QAHBZ6L1, FSCDJY67L3, QJSWX169M3, QJSWX200M1; among them, the typical colony of Lactobacillus crispatus is small, showing White, round, the colony is full in the middle and...

Embodiment 2

[0061] Embodiment 2: the cultivation of Lactobacillus crispatus

[0062] Pick the Lactobacillus crispatus (Lactobacillus crispatus) CCFM1110 single colony obtained in Example 1 and streak on the MRS solid medium containing 0.05g / L cysteine, after cultivating at 37°C for 48h, observe its colony and find its colony Small, white, round, the colony is full in the middle and diffuse around, which is a typical colony of Lactobacillus crispatus.

[0063] The bacterium colony of Lactobacillus crispatus (Lactobacillus crispatus) CCFM1110 obtained by cultivating carries out Gram staining (Gram staining method refers to textbook " Industrial Microbial Breeding " author: Zhuge Jian), and finds that it is a Gram-positive bacterium, in line with Typical characteristics of Lactobacillus crispatus.

[0064] Pick the Lactobacillus crispatus (Lactobacillus crispatus) CCFM1110 single bacterium colony obtained in Example 1 and insert it into the MRS liquid medium, after cultivating at 37° C. for...

Embodiment 3

[0068] Embodiment 3: the influence of different Lactobacillus crispatus on the viable count of Candida albicans

[0069] The experiment was divided into 6 groups, Lactobacillus crispatus CCFM1110 group, Lactobacillus crispatus QAHBZ6L1 group, Lactobacillus crispatus FSCDJY67L3 group, Lactobacillus crispatus QJSWX169M3 group, Lactobacillus crispatus QJSWX200M1 group and blank control group (Control group);

[0070] Among them, each group of Lactobacillus crispatus is as follows: take 100 μL of Lactobacillus crispatus and 100 μL of Candida albicans and inoculate them into a test tube containing 5 mL of MRS liquid medium, shake evenly, and culture the test tube in a 37°C incubator for 24 hours to obtain cultured The blank control group was as follows: inoculate 100 μL of MRS liquid medium and 100 μL of Candida albicans liquid into a test tube containing 5 mL of MRS liquid medium, shake evenly, and incubate the test tube in a 37°C incubator for 24 hours to obtain a culture solution...

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Abstract

The invention discloses a strain of Lactobacillus crispatus capable of preventing and / or treating candida vaginitis, and belongs to the technical field of microorganisms. The Lactobacillus crispatus CCFM1110 and / or its metabolites can prevent and / or treat Candida vaginitis, specifically: the Lactobacillus crispatus CCFM1110 can inhibit Candida albicans in vitro, and the cell-free supernatant of the Lactobacillus crispatus CCFM1110 Candida albicans can be inhibited in vitro. The cell-free supernatant of Lactobacillus crispatus CCFM1110 can inhibit the formation of Candida albicans biofilm in vitro. This Lactobacillus crispatus CCFM1110 can significantly reduce Candida albicans in the vagina of mice with vaginitis. Bacterial concentration, this Lactobacillus crispatus CCFM1110 can significantly reduce the number of Candida albicans hyphae in the vagina of Candida vaginitis mice, and this Lactobacillus crispatus CCFM1110 can significantly relieve the vaginal inflammation of Candida vaginitis mice.

Description

technical field [0001] The invention relates to a strain of Lactobacillus crispatus capable of preventing and / or treating candida vaginitis, and belongs to the technical field of microorganisms. Background technique [0002] Candida vaginitis (candida vaginitis) is a common fungal infection of the vaginal mucosa in women of childbearing age. According to statistics, about 75% of women in the world have at least one acute attack of candidal vaginitis in their lifetime, among which, 40% to 50% of women will have one or more recurrences, and 5% to 8% of women will have Developed into recurrent vulvovaginal candidiasis (RVVC), with 4 or more episodes within 1 year. [0003] The most common symptoms of patients with candidal vaginitis are excessive leucorrhea, burning and itching of the vulva and vagina, dysuria of the vulva, and geographic erythema of the vulva. Typical leucorrhea is in the form of curd or bean dregs. The vaginal mucosa is highly red and swollen. White thrush-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P15/02A61P31/10A61P31/02A61L15/40A61L15/46A61F13/15A61F13/20C12R1/225
CPCC12N1/20A61K35/747A61P15/02A61P31/10A61P31/02A61L15/40A61L15/46A61F13/15A61F13/202A61L2300/404C12R2001/225C12N1/205
Inventor 陈卫张秋香张丽丽张灏赵建新
Owner JIANGNAN UNIV
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