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Application of disulfiram in preparation of drug for treating liposarcoma

A technology of liposarcoma and disulfiram, applied in drug combination, anti-tumor drugs, pharmaceutical formulations, etc., can solve the problems of lack of treatment methods, easy recurrence and prognosis, and huge tumor volume.

Inactive Publication Date: 2020-08-11
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It is asymptomatic in the early stage of the disease. Therefore, when the patient is diagnosed, the tumor volume is often huge and has already invaded the abdominal viscera, making it difficult to completely remove it, resulting in easy recurrence and poor prognosis after surgery.
At present, the main treatment for this disease is surgical resection, and there is still a lack of other effective treatment methods. Therefore, it is urgent to find drugs that can inhibit retroperitoneal liposarcoma.

Method used

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  • Application of disulfiram in preparation of drug for treating liposarcoma
  • Application of disulfiram in preparation of drug for treating liposarcoma
  • Application of disulfiram in preparation of drug for treating liposarcoma

Examples

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Embodiment 1

[0031] 1.1 Drugs, cells, main reagents and instruments

[0032] The liposarcoma cell line SW872 was purchased from the American Culture Collection (ATCC); DMEM (Gibco, USA); fetal bovine serum (Sijiqing, China); trypsin (Hyclone, USA); double antibody (Hyclone, USA); Disulfiram, DSF) and ferroptosis inducer Erastin were purchased from APEBIO, USA; Fer-1, a ferroptosis inhibitor, was purchased from Sigma; CCK-8 kit (Bimake, USA); Giemsa (Solaibao, China); DAPI staining solution (China Keygen); double-stained apoptosis kit (GenStar, China); reverse transcription kit (Quanshijin, China); SBYR fluorescent quantitative PCR kit (Genstar, China); ALDH1, A20 and ACTB primers were provided by Beijing Sangong The primer synthesis department synthesized; A20, GAPDH antibody (CST, USA); horseradish peroxidase (HRP)-labeled goat anti-rabbit antibody (Zhongshan Jinqiao Company, China); RIPA lysate and protease inhibitor (Beyond, China). Class II biological safety cabinet (ESCO, USA); centr...

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Abstract

The invention provides an application of disulfiram in the preparation of a drug for treating liposarcoma, and relates to the technical field of drug research and development. The invention provides an application of disulfiram in the preparation of a drug for treating liposarcoma, an application in the preparation of a drug for inhibiting liposarcoma cell proliferation, an application in the preparation of a drug for promoting liposarcoma cell apoptosis, and an application in the preparation of a drug for inhibiting liposarcoma cell cloning. In the invention, disulfiram can inhibit the proliferation of the human liposarcoma cell line SW872 and induce the apoptosis of the human liposarcoma cell line SW872, a mechanism of disulfiram is related to the high expression of A20, but the mechanism is not related to ferroptosis, and DSF has the potential of inhibiting SW872 stem cells.

Description

technical field [0001] The invention belongs to the technical field of drug research and development, and in particular relates to the application of disulfiram in the preparation of drugs for treating liposarcoma. Background technique [0002] Retroperitoneal liposarcoma (RPLS) is the most common type of retroperitoneal sarcoma, accounting for about 45% of retroperitoneal soft tissue sarcomas. It is asymptomatic in the early stage of the disease. Therefore, when patients are diagnosed, the tumor volume is often huge and has invaded the abdominal viscera, making complete surgical resection difficult, resulting in easy recurrence and poor prognosis after surgery. At present, the main treatment for this disease is surgical resection, and there is still a lack of other effective treatment methods. Therefore, it is urgent to find drugs that can inhibit retroperitoneal liposarcoma. Contents of the invention [0003] In view of this, the object of the present invention is to pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/145A61P35/00
CPCA61K31/145A61P35/00
Inventor 王立生张林韩冰肖凤君苗成利
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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