Method of repairing skull defect models of New Zealand rabbits through autologous osteoblasts 3D bio-composite printed skull bone flap

Abstract

The invention discloses a method of repairing skull defect models of New Zealand rabbits through autologous osteoblasts 3D bio-composite printed skull bone flap, and belongs to the technical field ofskull repairing. The method can make printed skull bone flap precisely individualized so as to achieve the requirements of skull mechanics; cells can be uniformly distributed in the three-dimensionalspace of a support, the normal nutrition and metabolism of the cells can be maintained, and cell viability can be guaranteed; the printed simulated skull flap can be immediately transplanted to repaira skull defect model of a New Zealand rabbit; histopathology, cellular immunology, imageology and other methods can be applied to detect the activity of osteoblasts, the structural changes of the support and the fusion situations of skull bone flap and bone margin; the purposes of the method are to explore a brand-new biomaterial for skull defect repair and establish a novel treatment mode for clinical skull defect repair; and important experimental bases and theoretical bases can be provided for future 3D biological multi-material and multi-cell organ printing.

Description

technical field [0001] The invention belongs to the technical field of skull repair, in particular to a method for repairing a New Zealand rabbit skull defect model with 3D biocomposite printing of autologous osteoblasts. Background technique [0002] Skull defect refers to a disease with a series of clinical symptoms caused by craniocerebral injury, skull tumor and other craniocerebral surgery. There are more than 3 million patients with skull defects caused by accidents. With the continuous growth of the economic level and the continuous development of various undertakings, the life expectancy of the people has increased, and the incidence and detection of craniocerebral injuries, cerebrovascular diseases, and brain tumors have increased. The incidence rate of skull defects is increasing year by year, and the incidence of skull defects is also increasing year by year. [0003] (1) When repairing the skull defect of New Zealand rabbits, the most commonly used cranial repai...

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the above-mentioned defects of the prior art, the present invention provides a method for repairing the New Zealand rabbit skull defect model by 3D biocomposite printing of autologous osteoblasts, which solves the problem that 3D printing technology can only print simple bone tissue engineering scaffolds, and The non-degradable simulated skull is only used as a filling material for the skull defect and cannot be integrated with the autologous bone. The titanium alloy grid is repaired by manual bending of the titanium alloy grid, which is complicated to operate and takes a long time to shape. , The structure is not tightly combined, and at the same time, the repair effect mainly depends on the doctor's clinical experience and technical level, and it is not possible to carry out personalized processing and forming.