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USE OF EXTRACT OF VICTORIA CRUZIANA FOR preparing composition for improving gene expression quantity and improving moisturizing capability of skin

A technology of extracts and compositions, applied in the field of plant extracts, can solve the problems of harmfulness to human health, high price, etc.

Active Publication Date: 2020-08-04
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the known cosmetics, skin care products and health foods are made of chemical ingredients. Long-term use is not only harmful to human health, but also these products are often expensive and cannot be afforded by ordinary users

Method used

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  • USE OF EXTRACT OF VICTORIA CRUZIANA FOR preparing composition for improving gene expression quantity and improving moisturizing capability of skin
  • USE OF EXTRACT OF VICTORIA CRUZIANA FOR preparing composition for improving gene expression quantity and improving moisturizing capability of skin
  • USE OF EXTRACT OF VICTORIA CRUZIANA FOR preparing composition for improving gene expression quantity and improving moisturizing capability of skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of the extract of embodiment 1. Cruz king lotus

[0034] First, homogenize the flowers of Cruz King Lily (from Lianhe Country Leisure Lotus Garden, Baihe District, Tainan City) that have not bloomed, bloomed on the 1st day or bloomed on the 2nd day, and are then mixed with water, alcohols, and water-containing alcohols. or a combination thereof as an extraction solvent to extract the homogenized Cruz Regalia for 0.5 to 3 hours, wherein the extraction temperature is between 50° C. and 100° C., and the volume ratio of the extraction solvent to Cruz Reedia Cruz is 10 to 20:1 to 5. Next, cool the obtained product to room temperature, filter the product with a 400-mesh filter to obtain a filtrate, and then concentrate the filtrate under reduced pressure at 45-70° C. to obtain a concentrated product. Afterwards, the concentrated product is filtered with a filter core with a pore size of 5 μm to obtain the extract of the lotus cruz of the present invention.

Embodiment 2

[0035] Example 2. The detection of the total polyphenol content of the extract of King Lotus Cruz

[0036] First, prepare a standard solution and draw a standard curve, dissolve 10 mg of gallic acid (SigmaG7384) in water and add a volume of 10 mL to a volumetric flask, then store at -20°C as a stock solution. Afterwards, dilute the stock solution 10 times to a concentration of 100 μg / mL, and the unused liquid can be stored at -20°C. Next, gallic acid standard solutions of 0 μg / mL, 20 μg / mL, 40 μg / mL, 60 μg / mL, 80 μg / mL and 100 μg / mL were prepared respectively in glass test tubes. The preparation methods are shown in Table 1.

[0037] Table 1

[0038]

[0039] After that, add 500 μL of Folin-Ciocalteu's phenol reagent (Merck 1.09001.0100), mix well and let stand for 3 minutes, then add 400 μL of 7.5% sodium carbonate (Sigma 31432, dissolved in water) , Mix well and react for 30 minutes. Next, take 200 μL of standard solution to measure absorbance at 750 nm after ensuring ...

Embodiment 3

[0042] Example 3. Efficacy evaluation of the extract of King Lotus Cruz in improving the moisturizing ability of the skin

[0043] In this example, it is discussed whether the extract of the lotus cruz can improve the moisturizing ability of the skin by regulating the expression of genes related to skin cell moisturizing.

[0044] Human epidermal keratinocytes (HPEK-50; purchased from CELLnTEC) were cultured in keratinocyte-specific serum-free medium (Keratinocyte-SFM; purchased from Thermo, product number: 17005042) in a 6-well plate, the cell concentration of 2 mL medium 1.5×10 5 cells / well.

[0045] Afterwards, the cells were divided into 3 groups, including 1 control group and 2 experimental groups (ie, experimental groups 1 and 2). The extract of A. crusiensis that bloomed on the 1st day was diluted with a culture medium into dilutions having concentrations of 0.0625 mg / mL and 0.03125 mg / mL, and then 0.0625 mg / mL dilutions were added to the cells of experimental group 1...

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Abstract

The invention relates to the field of plant extracts, in particular to use of an extract of Victoria Cruziana for preparation of a composition for improving gene expression quantity and skin moisturizing capacity. The extract of the Victoria Cruziana is prepared by extracting the Victoria Cruziana by taking water, alcohol, hydrous alcohol or a combination thereof as an extraction solvent. The invention provides the use of the extract of the Victoria Cruziana to preparation of a composition for improving the expression quantity of keratin genes and hyaluronan synthetase 2 genes and improving the moisturizing ability of skin.

Description

technical field [0001] The present invention relates to the field of plant extracts, in particular to the use of the extract of A. crusiensis for preparing a composition for improving gene expression and skin moisturizing ability. Background technique [0002] The skin is the largest barrier to protect the human individual, and it has the function of resisting water loss, pathogenic bacteria and various environmental damages. Exposure to a large amount of ultraviolet rays (ultraviolet, UV), ionizing radiation, drugs or xenobiotics will promote the skin to generate reactive oxygen species (reactive oxygen species, ROS) and free radicals (free radicals). When the amount of accumulated reactive oxygen species and free radicals exceeds the antioxidant capacity of cells or tissues, oxidative stress will be formed. Then, reactive oxygen species and free radicals will react with intracellular components (including DNA, protein and lipids, etc.), and then have unwanted effects on t...

Claims

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Application Information

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IPC IPC(8): A61K36/62A61P17/16A61K8/9789A61Q19/00
CPCA61K36/62A61P17/16A61K8/9789A61Q19/00
Inventor 林咏翔李姿仪
Owner TCI CO LTD
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