3D printing method of bionic capillary network coated with multi-lamellar unit hydrogel

A capillary, 3D printing technology, applied in 3D culture, vascular endothelial cells, biochemical equipment and methods, etc., can solve the problems of poor mechanical strength, single shape, no environmental universality, etc., and achieve the effect of low cost

Active Publication Date: 2020-07-31
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to solve the problem that the hydrogel microfluidic vascular network product in the prior art has a single shape, does not fit the needs of the special organ blood vessels of the human body, does not have environmental universality, and the microfluidic vascular network has simple composition and low mechanical strength. Poor problem, provide a 3D printing method for biomimetic capillary network coated with multi-layer unit hydrogel

Method used

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  • 3D printing method of bionic capillary network coated with multi-lamellar unit hydrogel
  • 3D printing method of bionic capillary network coated with multi-lamellar unit hydrogel
  • 3D printing method of bionic capillary network coated with multi-lamellar unit hydrogel

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Experimental program
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Effect test

Embodiment 1

[0045] The 3D printing method of the biomimetic capillary network coated with multi-layer unit hydrogel, the preparation steps are as follows:

[0046] (1) Use 3D printing technology to prepare hydrogels containing vascular channels and growth factors in sheet units, specifically:

[0047] (1.1) To build a cylinder model, the specific process is: firstly dissolve gelatin in deionized water to prepare a 3D printing solution with a concentration of 10% (w / v), and then pour the 3D printing solution into the material tube of the 3D printing device , the constructed model is linearly printed layer by layer by the 3D printing device, and finally the printed product is freeze-dried, wherein the diameter of the cylinder is 0.05mm; the printing parameters are: injection pressure 1.0bar, printing speed 3.0mm / s, print nozzle diameter 0.26mm;

[0048] (1.2) Put the model obtained in step (1.1) into the injection molded container and then assemble it until all the cylinders are located a...

Embodiment 2

[0056] The 3D printing method of the biomimetic capillary network coated with multi-layer unit hydrogel, the preparation steps are as follows:

[0057] (1) Use 3D printing technology to prepare hydrogels containing vascular channels and growth factors in sheet units, specifically:

[0058] (1.1) Construct a composite model connected by two cylinders located at the same spatial level. The specific process is as follows: firstly, sodium alginate is dissolved in deionized water to prepare a 3D printing solution with a concentration of 20% (w / v). , and then pour the 3D printing solution into the material tube of the 3D printing device, and the 3D printing device will print the constructed model linearly layer by layer, and finally freeze-dry the printed product, wherein the diameter of the cylinder is 0.12 mm; printing parameters are: injection pressure 1.2bar, printing speed 3.8mm / s, printing nozzle diameter 0.45mm;

[0059] (1.2) Put the model obtained in step (1.1) into the in...

Embodiment 3

[0067] The 3D printing method of the biomimetic capillary network coated with multi-layer unit hydrogel, the preparation steps are as follows:

[0068] (1) Use 3D printing technology to prepare hydrogels containing vascular channels and growth factors in sheet units, specifically:

[0069] (1.1) Construct a composite model formed by connecting three cylinders located at the same spatial level. The specific process is as follows: firstly, chitosan is dissolved in 2% (w / w) glacial acetic acid to prepare a concentration of 20% ( w / v) 3D printing solution, and then pour the 3D printing solution into the material tube of the 3D printing device, and the constructed model is printed linearly layer by layer by the 3D printing device, and finally the printed product is freeze-dried, Among them, the diameter of the cylinder is 0.8mm; the printing parameters are: injection pressure 2.0bar, printing speed 5.2mm / s, printing nozzle diameter 0.8mm;

[0070] (1.2) Put the model obtained in s...

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Abstract

The invention relates to a 3D printing method of a bionic capillary network coated with multi-lamellar unit hydrogel. The method comprises the following steps: firstly, preparing hydrogel containing alamellar unit vascular channel and growth factors by utilizing a 3D printing technology, then inoculating endothelial cells into the channel, performing culturing for a period of time to obtain lamellar unit hydrogel vascular networks, then deforming and assembling the lamellar unit hydrogel vascular networks, adopting laser engraving or cutting and the like to obtain a to-be-repaired blood vessel part with a specific shape, and finally continuously performing culturing for a period of time to obtain the bionic capillary network coated with the multi-lamellar unit hydrogel. The lamellar unitvascular channel is of a planar human artery or vein blood vessel network-shaped structure and is composed of a plurality of cylindrical channels located on the same space layer or different space layers, the center distance between every two adjacent cylindrical channels is smaller than or equal to 5 mm, and the hydrogel can be invaded by endothelial cells and cells differentiated from the endothelial cells. The method disclosed by the invention is simple and feasible and has environmental universality.

Description

technical field [0001] The invention belongs to the technical field of hydrogel microfluidic vascular network, and relates to a 3D printing method for a bionic capillary network coated with multi-layer unit hydrogel. Background technique [0002] Microfluidics has now achieved precise fluid manipulation and extremely small volume control to meet challenges in the fields of high-throughput biomolecular analysis, disease diagnosis, and comprehensive cell research. In previous microfluidic systems, polydimethylsiloxane (PDMS) was used as a medium for fusion and fusion between cells. Due to its optical transparency, low toxicity, and biological inertness, it has been widely used in microfluidic gel systems. and chemical applications. However, PDMS microfluidic devices have the following disadvantages: [0003] (1) The PDMS microfluidic device is made of silicone prepolymer, which is affected by high-temperature processing during its preparation, making the components with biol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071B29C64/106B33Y10/00C08J3/075C08L89/00C08L5/04C08L5/08
CPCB33Y10/00B29C64/106C08J3/075C08J2305/04C08J2305/08C08J2389/00C12N5/0691C12N2513/00
Inventor 张耀鹏陈杰范苏娜黄利刘伟周广东王文波
Owner DONGHUA UNIV
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