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Preparation method and application of magnetic structural color hydrogel microcarrier for bladder cancer protein multi-element analysis

A technology of magnetic structure and multivariate analysis, applied in the field of biomedical materials, can solve the problems of poor repeatability, large steric hindrance, cross-contamination, etc., and achieve the effects of high specificity, good stability and high flexibility

Active Publication Date: 2020-07-24
NANJING DRUM TOWER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the shortcomings of traditional solid-phase chips with large steric hindrance, easy to cause cross-contamination, and poor repeatability, and to provide a preparation method and application of a magnetic structural color hydrogel microcarrier for multiple analysis of bladder cancer proteins , using photonic crystal microcarriers as templates to construct inverse opal scaffolds, prepare pregels containing magnetic nanoparticles and pour them into the connected pores of inverse opal scaffolds to prepare magnetic structural color hydrogel microcarriers

Method used

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  • Preparation method and application of magnetic structural color hydrogel microcarrier for bladder cancer protein multi-element analysis
  • Preparation method and application of magnetic structural color hydrogel microcarrier for bladder cancer protein multi-element analysis
  • Preparation method and application of magnetic structural color hydrogel microcarrier for bladder cancer protein multi-element analysis

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Embodiment 1

[0032] Preparation of magnetic structured color hydrogel microcarriers

[0033] (1) see figure 1 In the preparation process, silica nanoparticles with a particle size of 215nm were selected, and a monodisperse silica nanoparticle suspension with a concentration of 20m / v% was used as the internal phase, and dimethyl fluorinated silicone oil (50cSt) was used as the external phase. , the external phase and the internal phase are injected into the corresponding channels of the microfluidic device, collected in the container through the output channel of the microfluidic device, and at 65 ° C, the droplets of the silica nanoparticles evaporate with the water Self-assembled, solidified and crystallized for 12 hours to form silica photonic crystal microspheres; the temperature was raised to 95°C to solidify the silica photonic crystal microspheres for 2 hours; then the silica photonic crystal microspheres were collected in a crucible and washed with n-hexane The microspheres were cl...

Embodiment 2

[0038] Preparation of magnetic structured color hydrogel microcarriers

[0039] (1) see figure 1In the preparation process, silica nanoparticles with a particle size of 237nm were selected, and a monodisperse silica nanoparticle suspension with a concentration of 20m / v% was used as the internal phase, and dimethyl fluorinated silicone oil (50cSt) was used as the external phase. , the external phase and the internal phase are injected into the corresponding channels of the microfluidic device, collected in the container through the output channel of the microfluidic device, and at 65 ° C, the droplets of the silica nanoparticles evaporate with the water Self-assembled, solidified and crystallized for 12 hours to form silica photonic crystal microspheres; the temperature was raised to 95°C to solidify the silica photonic crystal microspheres for 2 hours; then the silica photonic crystal microspheres were collected in a crucible and washed with n-hexane The microspheres were cle...

Embodiment 3

[0043] Preparation of magnetic structured color hydrogel microcarriers

[0044] (1) see figure 1 In the preparation process, silica nanoparticles with a particle size of 247nm were selected, and a monodisperse silica nanoparticle suspension with a concentration of 20m / v% was used as the internal phase, and dimethyl fluorinated silicone oil (50cSt) was used as the external phase. , the external phase and the internal phase are injected into the corresponding channels of the microfluidic device, collected in the container through the output channel of the microfluidic device, and at 65 ° C, the droplets of the silica nanoparticles evaporate with the water Self-assembled, solidified and crystallized for 12 hours to form silica photonic crystal microspheres; the temperature was raised to 95°C to solidify the silica photonic crystal microspheres for 2 hours; then the silica photonic crystal microspheres were collected in a crucible and washed with n-hexane The microspheres were cl...

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Abstract

The invention discloses a preparation method and application of a magnetic structural color hydrogel microcarrier for bladder cancer protein multi-element analysis, and the method comprises the following steps: by using a silicon dioxide photonic crystal microstructure as a template, filling hydrogel with excellent mechanical properties in nano particle pores, curing, and removing the template toobtain a microcarrier with an inverse opal structure; and preparing functional pre-gel mixed with magnetic nanoparticles, pouring the functional pre-gel into gaps of the inverse opal structure, and carrying out ultraviolet polymerization to obtain the periodically ordered magnetic structure color hydrogel microcarrier similar to the template. The preparation method has the advantages of low preparation cost, good repeatability, simple operation and the like, and the prepared magnetic structural color hydrogel microcarrier has the characteristics of non-specific protein binding resistance, supply of a probe coupling active group, directional magnetic control effect and the like, has the properties of good coding stability, high flexibility and the like of a liquid phase chip, and also hasthe performance advantages of high sensitivity and high specificity when being used for multielement analysis of bladder cancer protein.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a preparation method and application of a magnetic structural color hydrogel microcarrier for multivariate analysis of bladder cancer proteins. Background technique [0002] Bladder cancer is the most common malignant tumor of the urinary system. Its morbidity and mortality rank among the top among common tumors worldwide, threatening life and health seriously. Bladder cancer has obvious multicentric characteristics and recurrence is common. Therefore, effective early diagnosis and prognostic monitoring methods are of great significance for the diagnosis and treatment of bladder cancer. Among them, the method of detecting tumor protein markers has the potential of predicting tumor progression and assessing the degree of tumor malignancy. [0003] At present, the commonly used methods for the quantitative determination of tumor protein markers in clinical laboratories include...

Claims

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Application Information

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IPC IPC(8): C08J9/42C08J9/40C08J9/26C08F122/14C08F2/48G01N33/574C08L35/02
CPCC08F2/48C08J9/26C08J9/40C08J9/405C08J9/42C08J2335/02C08J2451/08G01N33/57407G01N33/57484
Inventor 赵远锦魏晓巍王月桐
Owner NANJING DRUM TOWER HOSPITAL
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