A transcription factor ejcal involved in the regulation of loquat flower bud differentiation and its application
A transcription factor and flower bud differentiation technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of inability to distinguish, the late flowering time is greatly affected, and young jelly damage, to solve the young jelly damage and improve the yield of loquat. Effect
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[0017] This embodiment is the acquisition of the full-length cDNA sequence of the transcription factor EjCAL:
[0018] S1. Extraction of total RNA from loquat flower buds by improved CTAB method: Weigh 0.5 g of loquat flower buds stored at -80°C, grind them fully in liquid nitrogen, add to 4ml of 65°C preheated CTAB extraction buffer (containing 80 μl of β-mercaptoethanol) in a centrifuge tube, vortexed and heated at 65°C for 2 min; then add 4 ml of chloroform / isoamyl alcohol extract to the centrifuge tube (volume ratio of chloroform and isoamyl alcohol 24:1), vortex mixed, then centrifuged at 4°C and 10,000rpm for 10min, sucked up the supernatant, added 4ml of chloroform / isoamyl alcohol extract, and then re-centrifuged at 10,000rpm Centrifuge for 10min, draw the supernatant, add 1 / 4 volume of LiCl with a concentration of 10mol / l 2 , and then placed at a temperature of 4°C for 8h to 16h, the next day, centrifuged at a temperature of 4°C and a speed of 10,000rpm for 30min, dis...
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