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Human health and longevity markers based on specific peripheral blood DNA methylation sites and application of markers

A methylation and peripheral blood technology, applied in the field of biomedicine, can solve the problem that there are no biomarkers for evaluating human health and longevity, and achieve the effect of high accuracy and sensitivity

Inactive Publication Date: 2020-07-17
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these technologies are all developed based on the DNA methylation-age correlation of the general population, and there are currently no biomarkers and reference standards for human health and longevity assessment based on DNA methylation

Method used

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  • Human health and longevity markers based on specific peripheral blood DNA methylation sites and application of markers
  • Human health and longevity markers based on specific peripheral blood DNA methylation sites and application of markers
  • Human health and longevity markers based on specific peripheral blood DNA methylation sites and application of markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Human health and longevity markers based on specific peripheral blood DNA methylation sites

[0023] Based on 53 healthy and long-lived elderly people (age range of 100-111 years old, average age: 102.4 years old, life expectancy of more than 95 years old and no major senile diseases (the "Diseases of Critical Illness Insurance" implemented since April 3, 2007) (Definition and Use Specifications))) and 36 cases of control population (age range 45-71 years old, mean age: 59.3 years old) with matching living environment (same place of residence, similar eating habits) peripheral blood leukocyte DNA By comparing the methyl group data, 90 kinds of peripheral blood DNA methylation sites specific to long-lived people were obtained. These markers can be used alone or in combination of two or more to predict human health and longevity. Each DNA methylation The methylation level and changes of the methylation marker sites in the healthy and long-lived population, and i...

Embodiment 2

[0031] Example 2 Using specific peripheral blood DNA methylation sites as markers to predict health and longevity

[0032] Step 1. After the anticoagulated peripheral blood of the subject is collected according to a standardized and reasonable process, the genomic DNA of the whole blood is extracted, and the obtained DNA is subjected to whole-genome bisulfite treatment before DNA sequencing.

[0033] Step 2. Data processing: After the whole genome bisulfite sequencing, get off the computer to obtain the original fastq file, the fastq file is filtered and cleaned by Trimmomatic software with default parameters, and then compared to the human reference genome hg19 using Bismark, Bismark software will give The number of methylated cytosines at each site and the total coverage of the site, divided by the two is the methylation level of the site (ranging from 0% to 100%, that is, the unmethylated site : 0%; fully methylated sites: 100%).

[0034] The present invention utilizes Who...

Embodiment 3

[0035] Example 3 Taking the DNA methylation site chr14:81893209 in peripheral blood as an example, predicting health and longevity:

[0036] Steps 1-2 are consistent with Steps 1-2 of Example 2.

[0037] In step 3, the methylation level of chr14:81893209 of the subject is obtained as 80%. Looking up Table 1, it can be seen that the discrimination threshold is 51.83%, and the methylation level of the long-lived elderly should be lower than that of the control group, and the measured If the value of 80% is higher than the discrimination threshold of 51.83%, then the individual represented by this sample cannot achieve healthy and long life.

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Abstract

The invention discloses human health and longevity markers based on specific peripheral blood DNA methylation sites and an application of the markers. The total number of the human health and longevity markers is 90. A single, or two or more markers can be used for predicting human health and longevity, and the human health and longevity markers have the characteristics of high accuracy and high sensitivity. The AUC value of 14 sites exceeds 0.85, and thus the human health and longevity markers can be well used for evaluating the potential of individual health and longevity.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a human health and longevity marker based on a specific peripheral blood DNA methylation site and its application. Background technique [0002] The aging of the global population is an irreversible trend. my country has a large population base and the aging situation is even more severe. The elderly often suffer from one or even multiple senile diseases, and the health care costs of the elderly increase sharply with age. Statistics show that the health care expenditure of people over the age of 85 can be three times that of people aged 65-74. Therefore, early assessment of the healthy and long life potential of the elderly can play a role in early warning of health risks and help save public health costs. [0003] In the early days, the method for predicting human longevity potential was mainly based on the detection of DNA polymorphism sites, such as a patent...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/154C12Q2600/158
Inventor 孔庆鹏肖富辉王昊天
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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