Marker of lepidium latifolium and preparation method and application of marker

A technology of leaves and markers, which is applied to the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems that hinder the development and popularization of waffles, and achieve stable and controllable quality and preparation Simple process and wide distribution effect

Active Publication Date: 2020-07-17
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no information about the unique compounds in the quality of lentils, which affects the possibility of clear...

Method used

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  • Marker of lepidium latifolium and preparation method and application of marker
  • Marker of lepidium latifolium and preparation method and application of marker
  • Marker of lepidium latifolium and preparation method and application of marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A marker of L. latifolia, the structure of the marker is shown in the following formula (I):

[0045]

[0046] where: R 1 is glucosyl, R 2 From rhamnosyl.

[0047] Optionally, the structural formula of the marker is shown in the following formula (II):

[0048]

Embodiment 2

[0050] A preparation method of a marker of L. latifolia, comprising the following steps:

[0051] S1. Take 1 kg of lentils, dry them at 55° C. for 6 days, grind them, and sieve them to obtain 20-mesh coarse powder;

[0052] S2. The coarse powder in step S1 is extracted 3 times with 10L of ethanol with a concentration of 60% under ultrasonic conditions, and the extraction time is 2h each time and the obtained extracts are mixed, and then reduced at 70°C and 200hpa. Concentrate under pressure for 20 minutes to obtain the extract, and the ultrasonic condition is to react for 30 minutes under ultrasonic conditions of 40KHz and 600W;

[0053] S3, add 10 ~ 100mL methanol to the extract in step S2 to disperse, then add 20 ~ 200g silica gel to mix the sample, then go through silica gel chromatography after loading the sample, use dichloromethane: methanol: water (volume ratio 7: 3:0.5) elution, collect the eluate;

[0054] Concentrate under reduced pressure and then go through MCI r...

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Abstract

The invention belongs to the field of research of lepidium latifolium, and particularly relates to a marker of lepidium latifolium and a preparation method and application of the marker. The inventiondiscloses a marker shown as a formula (I). The marker provided by the invention is separated from lepidium latifolium. The method specifically comprises the following steps: extracting the lepidium latifolium by adopting a solvent, performing concentration under reduced pressure, performing eluting by silica gel column chromatography, performing eluting on line by MCI column chromatography, performing detection by high performance liquid chromatography, and preparing the marker by two-dimensional liquid chromatography. The invention also discloses a content range of the marker in the lepidiumlatifolium. The method is simple in process, high in preparation rate and easy for large-scale production.

Description

technical field [0001] The present invention belongs to the research field of waffles, and in particular relates to a marker of waffles and its preparation method and application. Background technique [0002] Lepidium latifolium L. is a plant of the genus Lepidium latifolium in the family Brassicaceae, also known as Lepidium latifolium L., with a height of 0.3-1.2m. Stems erect and glabrous, with branches in the upper part, slightly lignified at the base, hairy or glabrous, often with white powder. There are 4 white petals and 6 stamens. Siliques are short, broadly ovate or nearly round, glabrous and wingless. The fruiting period is July-October. In China, it is mainly distributed in Gansu, Qinghai, Ningxia, North China, Tibet and other places, and it is distributed nationwide. Lapis latifolia can treat visceral congestion, osteodystrophy and rheumatoid arthritis and the edema and hypertension caused by it. [0003] There are relatively few studies on the chemical cons...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08G01N30/02G01N30/06G01N30/54G01N30/74G01N30/90G01N30/94
CPCC07H1/08C07H17/07G01N30/02G01N30/06G01N30/54G01N30/74G01N30/90G01N30/94G01N2030/027G01N2030/062
Inventor 于瑞涛张玉佩吴楠王秀陶燕铎梅丽娟吴小可江磊王卫东
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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