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Compound feed for relieving liver glycogen accumulation of micropterus salmoides and preparation method of compound feed

A technology for compound feed and perch liver sugar is applied in the field of compound feed for relieving the accumulation of liver glycogen in largemouth bass and its preparation field, so as to achieve the effects of improving feeding performance, reducing accumulation and blood sugar content, and increasing starch content

Pending Publication Date: 2020-07-17
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Aiming at the problems such as liver damage and metabolic disorder caused by the sugar intolerance of largemouth bass raised with traditional feed, the main purpose of the present invention is to provide a compound feed for alleviating liver glycogen accumulation of largemouth bass, which can reduce the Glycogen accumulation in the liver improves its tolerance to sugar in feed

Method used

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  • Compound feed for relieving liver glycogen accumulation of micropterus salmoides and preparation method of compound feed
  • Compound feed for relieving liver glycogen accumulation of micropterus salmoides and preparation method of compound feed
  • Compound feed for relieving liver glycogen accumulation of micropterus salmoides and preparation method of compound feed

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The specific steps for preparing enzymatic starch are as follows:

[0056] Tapioca starch was selected as the starch source, and a 20% tapioca starch solution was prepared with sodium acetate buffer solution (pH 4.5). After thorough stirring, it was placed in an autoclave for gelatinization at 120°C for 10 minutes and cooled to room temperature to obtain a starch paste 化液. Then add pullulanase (1350NPUN / g) at the ratio of 6g / kg, enzymatically hydrolyze at 55℃, pH4.5 for 8h, then boil water bath for 10min to inactivate the enzyme, cool naturally and store at 4℃ for 24h, 60 Blast drying at ℃ for 26h, crushed and passed through 80 mesh sieve to obtain enzymolyzed starch.

[0057] It was determined that the amylose content of the obtained enzymatic starch was 28.21%, which was 30.0% higher than the tapioca starch before treatment; the resistant starch content was 14.22%, which was 299.4% higher than the tapioca starch before treatment.

Embodiment 2

[0059] Through gradient addition of α-cassava starch and the enzymatic starch prepared in Example 1 (addition amounts are 4%, 8% and 12% respectively), six kinds of compound feeds with equal nitrogen (51%) and fat (11%) are prepared. The formula is shown in Table 1. All low-fat raw materials are passed through an 80-mesh sieve, and are mixed uniformly according to the feed formula according to the step-by-step mixing method. The mixed soybean oil and soybean phospholipid are mixed with the above mixture evenly and passed through a 40-mesh sieve. A 40-mesh sieve was passed through the 2.5mm die hole of the granulator for granulation, then gelatinized in an oven at 105°C for 15 minutes, and dried in an oven at 55°C. After drying, store the feed in a refrigerator at -20°C for later use.

[0060] Table 1: Experimental feed formula

[0061]

[0062] The present invention also provides the application of the enzymatic starch prepared in Example 1 in reducing liver glycogen accumulation...

Embodiment 3

[0064] Using the compound feed prepared in Example 2, the largemouth bass with an initial weight of 13.08±0.02g was selected as the experimental object, and a 12-week breeding experiment was carried out in a recirculating aquaculture system, with 3 replicates for each treatment and 40 for each replicate. Tail fish. The effects on growth, visceral index, liver glycogen content, postprandial blood glucose and liver histology of largemouth bass were determined. The experimental data is expressed by the mean ± standard error, and SPSS19.0 analysis software is used for two-way analysis of variance. The significance of the difference between fixed factors is determined by Turkey's test method, and P <0.05 is the significant level.

[0065] Table 2: Experimental breeding results of each group

[0066]

[0067] Note: In the two-factor analysis, n.s. means that the difference is not significant; the difference between starch types is significant with *.

[0068] It can be seen from Table 2 ...

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Abstract

The invention discloses a compound feed for relieving liver glycogen accumulation of micropterus salmoides and a preparation method of the compound feed. The compound feed is prepared by uniformly mixing 88-92% by weight of a basal feed and 8-12% by weight of enzymolysis starch step by step, adding water for kneading, and performing granulation, gelatinization and drying. The basal feed comprisesthe following components in parts by mass: 42 parts of fish meal, 3 parts of vital gluten, 4 parts of blood meal, 5 parts of shrimp meal, 9 parts of fermented soybean meal, 11 parts of corn protein powder, 2 parts of squid powder, 2 parts of beer yeast, 3 parts of soybean phospholipid, 1 part of a vitamin mixture, 1 part of a mineral substance mixture, 1 part of monocalcium phosphate and 4 parts of soybean oil, or the compound feed also comprises 1-4 parts of zeolite powder, wherein the amylose content of the enzymolysis starch is not lower than 28.21%, and the resistant starch content is notlower than 14.22%. The compound feed can reduce liver glycogen accumulation of micropterus salmoides, improve sugar tolerance of micropterus salmoides, and solve the problems of liver injury and metabolic disturbance caused by sugar intolerance of micropterus salmoides fed traditional feed.

Description

Technical field [0001] The invention belongs to the technical field of aquatic animal feed, and specifically relates to a compound feed for alleviating the accumulation of liver glycogen of largemouth bass and a preparation method thereof. Background technique [0002] Largemouth bass is an important farmed economic fish in my country. In 2018, its output has reached 450,000 tons and is showing a continuous growth trend. Although domestic and foreign researchers have made some progress in the study of the nutrient requirements of largemouth bass, the special compound feed for largemouth bass based on this is likely to cause liver disease of largemouth bass in the middle and late stages of breeding, which will lead to its growth performance and feed. Reduction in utilization. At present, the cultivation of largemouth bass still relies on the use of chilled juvenile trash fish, which will not only cause a waste of marine fishery resources, but also seriously restrict the sustainab...

Claims

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Application Information

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IPC IPC(8): A23K10/22A23K10/24A23K10/12A23K10/14A23K10/37A23K20/147A23K20/158A23K20/163A23K20/174A23K20/26A23K20/20A23K20/24A23K40/10A23K50/80C12P19/04C12P19/16
CPCA23K10/22A23K10/24A23K10/12A23K10/14A23K10/37A23K20/147A23K20/158A23K20/163A23K20/174A23K20/26A23K20/20A23K20/30A23K20/24A23K40/10A23K50/80C12P19/04C12P19/16Y02A40/818Y02P60/87
Inventor 李松林陈乃松桑春燕张佳灿韩志豪
Owner SHANGHAI OCEAN UNIV
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