Field environment nitrogen fixation activity ARA determination method and device

A measurement method and nitrogen fixation technology, which are applied in measurement devices, instruments, scientific instruments, etc., can solve the problem that the measurement results cannot accurately reflect the actual situation of complex environmental changes in the field, and achieve easy portability, expansion of the test range, and strong adaptability. Effect

Pending Publication Date: 2020-07-14
NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the invention of the present invention is to: aim at the above-mentioned existing problems, provide field environment nitrogen fixation activity ARA measurement method and device, through the mode of field in situ observation research, cooperate self-designed simple scientific research instrument, utilize this scientific research instrument in the field environment Study the nitrogen fixation activity of soil nitrogen-fixing microorganisms in response to hydrothermal conditions and complex and variable environmental factors, and then can obtain accurate field in situ measurement results, which are more in line with the actual situation in the field environment, and solve the problem that the existing ARA measurement methods can only be used in Determination in the laboratory, and the measurement results cannot accurately reflect the actual situation of complex environmental changes in the field

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  • Field environment nitrogen fixation activity ARA determination method and device
  • Field environment nitrogen fixation activity ARA determination method and device
  • Field environment nitrogen fixation activity ARA determination method and device

Examples

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Embodiment 1

[0048] Taking biological soil crusts (BSCs, hereinafter referred to as biological crusts) widely distributed in desert ecosystems as an example, three types of typical biological soil crusts (algae crusts, lichen crusts, and moss crusts) with nitrogen fixation activity were selected under field conditions. ), the distilled water equivalent to 3mm rainfall was evenly sprayed on the surface of the crust before the test, and then the plexiglass cover (12cm in diameter, 10cm in height, open at the bottom, closed at the top, leaving a round hole in the middle, sealed with a rubber stopper) was covered in the On the crust, the bottom end is inserted into the soil 3cm to form a closed space. Use the above-mentioned acetylene filling device, open the pressure reducing valve 5 on the acetylene tank 1, open the one-way valve 4, and select a suitable gas collector 3 as required Extract acetylene gas through the openable one-way valve 4 at the end of the gas collector 3 (that is, the extra...

Embodiment 2

[0053] In order to further clarify whether the difference between the laboratory measurement and the field in situ measurement is caused by destructive sampling, we arranged the crust samples collected in Example 1 in the laboratory and after the same moisture treatment in the outdoor natural environment, respectively. After adding acetylene gas using the above-mentioned acetylene filling device, repeat the test process in the above-mentioned Example 1, and the specific measured data are shown in Table 2.

[0054] Table 2 Nitrogen fixation activity of three typical biological soil crusts in outdoor environment and laboratory

[0055]

[0056] It can be obtained from Table 2 that the nitrogen fixation activity of Type 3 crusts is similar to Example 1, the indoor test results are close to Example 1, and the overall data of outdoor tests are lower than Example 1, indicating that destructive sampling will affect the test results, mainly because the samples were After the distur...

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Abstract

The invention discloses a field environment nitrogen fixation activity ARA determination method and device. The field environment nitrogen fixation activity ARA determination method comprises the following steps: S1, selecting biological soil crusts (hereinafter referred to as biological crusts) with nitrogen fixation activity under a field environment condition; s2, spraying distilled water to the biological crust, and then covering the biological crust with a glass cover to form a closed space; S3, injecting acetylene gas into the glass cover by using an acetylene injection device, and thenculturing for more than 3 hours; S4, after the culture is finished, extracting the gas in the glass cover by using an extraction device, then analyzing the gas by using a gas chromatographic analysisinstrument, and finally performing measurement to obtain data. A field in-situ observation and research mode is adopted; the field in-situ measurement result is obtained by matching with a self-designed simple and convenient scientific research instrument, the measurement result better conforms to the actual situation of the field environment, and the problems that an existing ARA measurement method can only perform measurement in a laboratory and the measurement result cannot accurately reflect the actual situation of field complex environment change are solved.

Description

technical field [0001] The invention relates to the technical field of measuring nitrogen-fixing activity ARA, in particular to a method and device for measuring nitrogen-fixing activity ARA in a field environment. Background technique [0002] Nitrogen (N) availability is generally considered to limit ecological processes in terrestrial ecosystems, and explicit consideration of nitrogen biogeochemical processes, including biological nitrogen fixation, is critical for understanding ecosystem responses to environmental change. In terrestrial ecosystems, free-living nitrogen fixation processes that occur on a variety of substrates are almost ubiquitous and often represent a major route to bioavailable nitrogen sources, yet their importance is often underestimated. Nitrogen to nitrogen (N 2 ) in the atmosphere in a form that is not available to vascular plants. N 2 It must first be "fixed" or "reduced" by eukaryotes such as eubacteria and cyanobacteria to ammonia (NH + ) fo...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张鹏何明珠黄磊王进杨昊天宋光赵杰才
Owner NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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