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Davidia involucrata seedling and davidia involucrata tissue culture method

A tissue culture, dove tree technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems that seeds are difficult to develop into seedlings, the endocarp of dove tree is hard, and the dove tree is difficult to be widely used, etc. Wide range of objects, faster reproduction, and better startup effects

Pending Publication Date: 2020-07-14
SHAANXI SCI TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, the endocarp of Davidia involucrata is hard, and the phenomenon of seed abortion is serious. Although there is no inhibitory substance in the embryo, it is difficult for the seeds to break through the pericarp and develop into seedlings.
Based on the above reasons, Davidia involucrata is difficult to be widely used in landscaping

Method used

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  • Davidia involucrata seedling and davidia involucrata tissue culture method
  • Davidia involucrata seedling and davidia involucrata tissue culture method
  • Davidia involucrata seedling and davidia involucrata tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for culturing Davidia involucrata tissue, comprising the following content:

[0040] (1) Disinfection of explants

[0041] Select the leaves of Davidia involucrata as spare explants, first soak the spare explants with 800 times of carbendazim for 15 minutes, take out and rinse with water for 15 minutes; soak the washed explants in 70% alcohol for 50 minutes, and then place them in a concentration of 0.02% sodium hypochlorite solution for 5 minutes, take it out and wash it with sterile water, and finally place it in 0.1% brogeramine solution for 10 minutes, take it out and wash it with sterile water several times, remove the water and set it aside, it is the explant for tissue culture body;

[0042] (2) Induction culture

[0043] The explants treated in (1) are placed in the induction medium and cultured, and the induction medium is MS medium containing vitamins, naphthaleneacetic acid, kinetin and sucrose, wherein vitamins, naphthaleneacetic acid, kinetin and...

Embodiment 2

[0049] A method for culturing Davidia involucrata tissue, comprising the following contents:

[0050] (1) Disinfection of explants

[0051] Select the winter buds of Davidia involucrata as spare explants, first soak the spare explants with 1000 times of carbendazim for 20 minutes, take out and rinse with water for 20 minutes; soak the washed explants in 70% alcohol for 70 seconds, and then place them in a concentration of 0.03% sodium hypochlorite solution for 8 minutes, take it out and wash it with sterile water, and finally place it in 0.15% brogeramine solution for 14 minutes, take it out and wash it with sterile water several times, remove the water and set it aside, that is, the explant for tissue culture body;

[0052] (2) Induction culture

[0053] The explants treated in (1) are placed in the induction medium and cultured, and the induction medium is MS medium containing vitamins, naphthaleneacetic acid, kinetin and sucrose, wherein vitamins, naphthaleneacetic acid, ...

Embodiment 3

[0059] A method for culturing Davidia involucrata tissue, comprising the following contents:

[0060] (1) Disinfection of explants

[0061] Select the tender shoots of Davidia involucrata as spare explants, first soak the spare explants with 1500 times of carbendazim for 30 minutes, take out and rinse with water for 25 minutes; soak the washed explants in 75% alcohol for 80 seconds, and then place them in the concentration 0.04% sodium hypochlorite solution for 10 minutes, take it out and wash it with sterile water, and finally place it in 0.2% brogeramine solution for 15 minutes, take it out and wash it with sterile water for several times, remove the water and set it aside, that is, the outer tube for tissue culture Implant;

[0062] (2) Induction culture

[0063] The explants treated in (1) are placed in the induction medium and cultured, and the induction medium is MS medium containing vitamins, naphthaleneacetic acid, kinetin and sucrose, wherein vitamins, naphthaleneac...

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Abstract

The invention provides a davidia involucrata tissue culture method. The davidia involucrata tissue culture method comprises the following contents of: (1) explant disinfection: selecting reserve explants of davidia involucrata, carrying out disinfection treatment on the reserve explants by using a disinfectant as explants for tissue culture after disinfection is completed; (2) inducing culture: culturing the explants treated in the step (1) in an inducing medium to obtain primary cultured seedlings; (3) subculture: culturing the primary cultured seedlings treated in the step (2) into a subculture medium to obtain subcultured buds; and (4) strong seedling culture and rooting culture: culturing the subcultured buds obtained in the step (3) in a strong seedling medium, culturing the subcultured buds in a rooting medium after culturing is completed, and obtaining davidia involucrata seedlings after culturing is completed. The tissue culture method provided by the invention has the advantages of being relatively low in contamination rate, good in initiating effect and high in induction differentiation ratio of culture initiating, and tissue cultured seedlings grow well.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a method for tissue culture of Davidia involucrata and a Davidia involucrata seedling obtained by tissue culture. [0002] technical background [0003] Davidia involucrata is a plant of the genus Davidia involucrata, which is a unique genus in my country. It has a variety of Davidia involucrata and Davidinia glabrata. It is a relic species of the Tertiary ancient tropical flora and is listed as a national rare and endangered plant. , known as "living fossil". Davidia involucrata is a large deciduous tree with a broad oval or conical crown and a beautiful tree shape; the head of the inflorescence is brown and covered with two bracts of different sizes. When the inflorescence matures, the color of the bracts changes from green to white. It has a very high ornamental value. As early as 1986, Davidia involucrata attracted the attention of foreign botanists. At the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李双杜建括
Owner SHAANXI SCI TECH UNIV
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