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Method for preparing saussurea involucrata cell extract by using multi-component liquid system and use of saussurea involucrata cell extract

A technology for extracts and snow lotus, which is applied in the field of preparing snow lotus cell extracts in a multi-component liquid system, which can solve the problems of increasing the cost of use and darkening the color of the extract

Pending Publication Date: 2020-07-07
安赛搏(重庆)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the existing patents use the method of water extraction or ethanol extraction to extract the effective active ingredients from the Tianshan Snow Lotus cells, but the water extraction process will easily lead to the darkening of the extract, and the water extract contains a large amount of sugar, which is used in cosmetics. During the production process, it is susceptible to the influence of polyols, resulting in precipitation; and ethanol extracts, due to the irritation of ethanol itself, are limited in use in the cosmetic production process. Usually, ethanol reagents contain methanol, which must be tested before use. Methanol content test increases the cost of using the product

Method used

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  • Method for preparing saussurea involucrata cell extract by using multi-component liquid system and use of saussurea involucrata cell extract
  • Method for preparing saussurea involucrata cell extract by using multi-component liquid system and use of saussurea involucrata cell extract
  • Method for preparing saussurea involucrata cell extract by using multi-component liquid system and use of saussurea involucrata cell extract

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Effect test

Embodiment 1

[0085] The method for preparing Saussurea sauraceae cell extract by multiple liquid system:

[0086] Accurately weigh 10g of fresh Saussurea cerevisiae cells, add 50mL of water, ethanol, butylene glycol, propylene glycol, multi-solvent water: butanediol: propylene glycol = 5:2:3 (m:m:m) to extract , the extraction temperature is 100°C (the ethanol is boiling at 76°C), the extraction time is 1h, and then centrifuged at 6500rpm for 10min, and the supernatant is retained, which is the cell extract of Snow Lotus.

Embodiment 2

[0088] Accurately weigh 10 g of fresh Saussurea sauraceae cells, add 30 mL of multi-solvent for extraction respectively, the mass ratios are water: butanediol: propylene glycol = 1:1:1 (m:m:m), and extract, extract The temperature is 100°C (the ethanol is boiling at 76°C), the extraction time is 1h, and then centrifuged at 6500rpm for 10min, and the supernatant is retained, which is the cell extract of Snow Lotus Tianshan

Embodiment 3

[0090] Accurately weigh 10g of snow lotus stem cells, add 60mL of multi-solvent to extract respectively, the mass ratio of multi-solvent is water:butanediol:propylene glycol=1:2:3 (m:m:m), leaching at room temperature for 24h, After that, centrifuge at 6500 rpm for 10 minutes, and keep the supernatant, which is the cell extract of Snow Lotus Tianshan.

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Abstract

The present invention discloses a method for preparing a saussurea involucrata cell extract by using a multi-component liquid system. The multi-component liquid system of water, butanediol, and propylene glycol is mainly used to extract effective components in saussurea involucrata cells. The butanediol specifically refers to 1,3-butanediol and the propylene glycol refers to 1,3-propylene glycol;and in the multi-component liquid system, a concentration range of the butanediol is 0-70%, content of the propylene glycol is 0-70% and content of the water is 20-90%. The method avoids disadvantagescaused by complete water extraction and ethanol extraction, increases stability of the product, improves quality of the product, and at the same time, enables the saussurea involucrata cells to be more prominent in skin-care effects in cosmetics due to skin-care effects of the butanediol and propylene glycol.

Description

technical field [0001] The invention belongs to the field of plant extraction biotechnology, and relates to a method for preparing a Saussurea oleracea cell extract by a multi-component liquid system and its application. Background technique [0002] Tianshan snow lotus is distributed in the north and south slopes of Tianshan Mountains, Altai Mountains and Kunlun Mountains in Xinjiang, China, at an altitude of 4000-5000 meters. It is an excellent Chinese medicinal material, which is effective for irregular menstruation, toothache, rheumatoid arthritis, impotence, abnormal leucorrhea, and metrorrhagia. Snow lotus seeds germinate at zero degrees Celsius and grow at 3-5°C, and the seedlings can withstand the severe cold of −21°C. Although it takes 5 years to grow to flower, in fact the main growth period is only 8 months. In recent years, due to excessive harvesting and digging, the number of snow lotus has been greatly reduced, and it is a national endangered second-class pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/9789A61K8/34A61Q19/00A61Q19/08A61P29/00
CPCA61K8/99A61K8/9789A61K8/345A61Q19/00A61Q19/08A61P29/00A61K2800/524A61K2800/82A61K2800/805
Inventor 宋静静陈萌马原王军刘磊张卫
Owner 安赛搏(重庆)生物技术有限公司
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