Pulse stirring type bioreactor for secretion, separation and collection of exosomes

Abstract

The present invention discloses a pulse stirring bioreactor for secretion, separation and collection of exosomes. The pulse stirring bioreactor comprises a cell culture device and an exosome separation and collection device; the exosome separation and collection device is connected with the cell culture device and can collect the exosomes separated from a culture solution in the cell culture device; the cell culture device comprises a stirring device and is used for applying stirring power to the culture solution in the cell culture device; and the cell culture device comprises a pulse devicewhich can add the culture solution to the cell culture device in a pulsed manner. The bioreactor can enable the culture solution and cells to be fully mixed to keep enough oxygen supply, the stirringdevice is not in direct contact with the cells and reduces a shearing force of the cells, and at the same time, the pulse stirring bioreactor can simulate cell growth environment in vivo and stimulateNK cells or other suspended cells to secrete a large amount of cell products

Description

technical field [0001] The invention relates to the preparation and separation technology of exosomes, in particular to a pulse stirring bioreactor for exosome secretion, separation and collection. Background technique [0002] Exosomes are extracellular vesicles (EVs) with a lipid bilayer and a diameter of about 30-150 nm. Exosomes can contain a variety of biologically active substances such as proteins, lipids, and nucleic acids. Most cells in the human body, such as immune cells (T cells, B cells, Kupffer cells, and NK cells), endothelial cells, and platelets, can secrete exosomes. body. At present, the research on exosomes in clinical treatment can be roughly summarized as follows: (1) regulating the release and distribution of exosomes. (2) Exosome-based drug delivery. (3) Exosome-mediated tumor targeting therapy. (4) Exosomes and immunotherapy. At present, exosomes have shown great application prospects in the immunotherapy of tuberculosis, in the diagnosis, inhib...

AI Technical Summary

Problems solved by technology

The disadvantage is that it is difficult to ensure the absorption efficiency of oxygen

In recent years, there is also a method of using air or oxygen injection to solve the oxygen supply in the culture medium, but this method is prone to generate a large amount of foam, and the cells will be physically damaged during the foam bursting process, and excessive oxygen injection will also poison the cells.

Precise control of gas injection also complicates reactor equipment, increasing the cost of cell culture

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