ARTIFICIAL beta-CELLS AND METHODS OF USE THEREOF

Abstract

Disclosed herein is a particle containing an inner liposomal vesicle (ILV) encapsulating a therapeutic agent; an outer liposomal vesicle (OLV) encapsulating the ILV; a membrane fusion-promoting agent;and a pH-altering agent. Also disclosed are methods of delivering a therapeutic agent to a subject comprising: a) providing a herein disclosed particle b) triggering ILV and OLV fusion; and c) releasing the therapeutic agent outside of the OLV. Also disclosed are methods for treating a disease in a subject in need thereof comprising: administering to a subject a herein disclosed particle. Also disclosed are methods to release insulin to an environment comprising increased glucose levels, the method comprising exposing to the environment a herein disclosed particle.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Patent Application Serial No. 62 / 559,909 filed September 18, 2017, the disclosure of which is expressly incorporated herein by reference in its entirety. technical field [0003] The disclosure herein relates to granules, methods for delivering therapeutic agents, and methods for treating diseases such as diabetes. Background technique [0004] Pancreatic β cells dynamically regulate insulin secretion to maintain blood glucose homeostasis. Destruction or dysfunction of these cells leads to type 1 and type 2 diabetes, a family of chronic diseases that currently affect more than 415 million people in the world (Rorsman, P., Annu. Rev. Physiol. 75, 155-179( 2013); Yu, J. et al., Proc. Natl Acad. Sci. USA 112, 8260-8265 (2015); Ohkubo, Y. et al., Diabetes. Res. Clin. Pract. 28, 103-117 (1995)). Insufficient glycemic control due to loss of β-cell function can lead to...

AI Technical Summary

Problems solved by technology

Although multi-compartmental assemblies have been created to recreate the hierarchical architecture of cells for the delivery of drug mixtures or cascade reactions (Boyer, C. et al., ACS Nano 1, 176-182 (2007); Wong, B. et al. , Adv.Mater.23, 2320-2325 (2011); Marguet, M. et al., Angew.Chem.Int.Ed.51, 1173-1176 (2012); Peters, R. et al., Angew.Chem.Int. .Ed.53, 146-150 (2014); Elani, Y. et al., Nat.Commun.5, 5305-5309 (2014); Chiu, H. et al., Angew.Chem.Int.Ed.47, 1875-1878 (2008)), but replication of native cellular 'sense-response' behavior remains elusive

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