Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Low-cost, high-biomass and high-protein-content chlorella pyrenoidosa culture method

A technology of high biomass of chlorella pyrenoidosa, applied in the biological field, can solve the problems of high production cost of chlorella pyrenoidosa, and achieve the effects of high market competitiveness, high protein content, and high biomass

Active Publication Date: 2020-06-30
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
View PDF9 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem of high production cost of Chlorella pyrenoidosa at present, the present invention provides a low-cost, high-biomass and high-protein Chlorella pyrenoidosa culture method; Pot method" in situ saccharification of straw, and using the straw saccharification solution as a cheap and high-quality carbon source, and cultivating Chlorella pyrenoidosa in an adjunct culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Low-cost, high-biomass and high-protein-content chlorella pyrenoidosa culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for cultivating Chlorella pyrenoidosa with low cost, high biomass and high protein content, comprising the following steps:

[0024](1) Activation of Clostridium thermocellum strains: Inoculate 100ul of β-1,4-glucosidase into the recombinant strain of Clostridium thermocellum DSM 1313 cellulite into 4ml of anaerobic fermentation medium, activate at 50°C After 18 hours, it was directly used as a saccharification strain after reactivation. The composition of the anaerobic fermentation medium is: dipotassium hydrogen phosphate 2.9g / L, potassium dihydrogen phosphate 1.5g / L, urea 2.1g / L, calcium chloride 0.1g / L, magnesium chloride 1.8g / L, sulfuric acid Ferrous 0.0005g / L, sodium sulfide 2g / L, corn steep liquor 4g / L, trisodium citrate 2g / L, pH 6.5-7.5.

[0025] (2) Straw saccharification: transfer the pretreated straw to the anaerobic fermentation system, add medium according to the solid-to-liquid mass ratio of 1:10, add an appropriate amount of the mutagenized stra...

Embodiment 2

[0033] Different from Example 1, a method for cultivating Chlorella pyrenoidosa with low cost, high biomass and high protein content comprises the following steps:

[0034] (1) Activation of Clostridium thermocellum strain: Inoculate 150ul of β-1,4-glucosidase into the recombinant strain of Clostridium thermocellum DSM 1313 cellulite into 4ml of anaerobic fermentation medium, activate at 50°C After 18 hours, it was directly used as a saccharification strain after reactivation.

[0035] (2) Straw saccharification: transfer the pretreated straw to the anaerobic fermentation system, add medium according to the solid-to-liquid mass ratio of 1:12, add an appropriate amount of the mutagenized strain obtained in step (1) into the anaerobic fermentation tank, The straw saccharification was carried out at a temperature of 53° C. to obtain a saccharification solution mainly composed of glucose, and the concentration of glucose measured by high performance liquid chromatography reached 6...

Embodiment 3

[0039] Different from Example 1, a method for cultivating Chlorella pyrenoidosa with low cost, high biomass and high protein content comprises the following steps:

[0040] (1) Activation of Clostridium thermocellum strain: Inoculate 120ul of β-1,4-glucosidase into the recombinant strain of Clostridium thermocellum DSM 1313 cellulite into 4ml of anaerobic fermentation medium, activate at 50°C After 18 hours, it was directly used as a saccharification strain after reactivation.

[0041] (2) Straw saccharification: transfer the pretreated straw to the anaerobic fermentation system, add medium according to the solid-to-liquid mass ratio of 1:14, add an appropriate amount of the mutagenized strain obtained in step (1) into the anaerobic fermentation tank, Saccharification of straw was carried out at a temperature of 55°C to obtain a saccharification solution mainly composed of glucose, and the concentration of glucose measured by high performance liquid chromatography reached 64g / ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a low-cost, high-biomass and high-protein-content chlorella pyrenoidosa culture method. The method specifically comprises the steps of carrying out straw saccharification on recombinant strains, and carrying out mixotrophic culture on chlorella by taking straw saccharification liquid as a cheap raw material. The method comprises the following steps of: (1) activating recombinant strains of clostridium thermocellum; (2) carrying out straw saccharification: transferring pretreated straws into an anaerobic fermentation system, adding a culture medium and the recombinant strains, and carrying out straw saccharification; (3) preparing a chlorella pyrenoidosa culture solution; and (4) carrying out mixotrophic culture on chlorella pyrenoidosa: inoculating a proper amount ofchlorella species into the culture solution, and carrying out mixotrophic culture under proper light intensity. According to the method, the ability of degrading lignocellulose to produce glucose byclostridium thermocellum fiber bodies is further improved through the recombinant strains of clostridium thermocellum; and the chlorella pyrenoidosa obtained through mixotrophic culture is high in biomass and high in protein content, so that the production cost of chlorella is reduced, and the market competitiveness of chlorella pyrenoidosa and derivative products thereof is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a recombinant strain that integrates β-1,4-glucosidase into the cellulosome of Clostridium thermocellum DSM 1313 to carry out straw saccharification, and uses straw saccharification liquid as a cheap raw material for simultaneous cultivation Chlorella approach. Background technique [0002] The output of crop straw is huge, and the annual output of Shandong Province alone is as high as more than 70 million tons. Among them, about 15 million tons of straw are directly burned without utilization, which not only pollutes the environment, but also causes a great waste of cellulose resources. The main component of straw is cellulose, which can be hydrolyzed with cellulase to produce a high-concentration sugar solution, which can be used as a cheap sugar source for microalgae cultivation. [0003] Chlorella pyrenoidosa is recognized as "green gold" in the world. It is a high-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/12C12N1/21C12P19/14C12P19/02C12R1/89C12R1/145
CPCC12N1/12C12N1/20C12N9/2445C12P19/14C12P19/02C12Y302/01021
Inventor 崔球肖艳颜飞祁宽
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com