Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for culturing Chlorella pyrenoidosa with high biomass and high protein content

A technology of high biomass of chlorella pyrenoidosa, applied in the biological field, can solve the problems of high production cost of chlorella pyrenoidosa, and achieve the effects of high market competitiveness, high biomass and high protein content

Active Publication Date: 2022-04-15
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem of high production cost of Chlorella pyrenoidosa at present, the present invention provides a low-cost, high-biomass and high-protein Chlorella pyrenoidosa culture method; Pot method" in situ saccharification of straw, and using the straw saccharification solution as a cheap and high-quality carbon source, and cultivating Chlorella pyrenoidosa in an adjunct culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for culturing Chlorella pyrenoidosa with high biomass and high protein content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for cultivating Chlorella pyrenoidosa with low cost, high biomass and high protein content, comprising the following steps:

[0024](1) Activation of Clostridium thermocellum strains: Inoculate 100ul of β-1,4-glucosidase into the recombinant strain of Clostridium thermocellum DSM 1313 cellulite into 4ml of anaerobic fermentation medium, activate at 50°C After 18 hours, it was directly used as a saccharification strain after reactivation. The composition of the anaerobic fermentation medium is: dipotassium hydrogen phosphate 2.9g / L, potassium dihydrogen phosphate 1.5g / L, urea 2.1g / L, calcium chloride 0.1g / L, magnesium chloride 1.8g / L, sulfuric acid Ferrous 0.0005g / L, sodium sulfide 2g / L, corn steep liquor 4g / L, trisodium citrate 2g / L, pH 6.5-7.5.

[0025] (2) Straw saccharification: transfer the pretreated straw to the anaerobic fermentation system, add medium according to the solid-to-liquid mass ratio of 1:10, add an appropriate amount of the mutagenized stra...

Embodiment 2

[0033] Different from Example 1, a method for cultivating Chlorella pyrenoidosa with low cost, high biomass and high protein content comprises the following steps:

[0034] (1) Activation of Clostridium thermocellum strain: Inoculate 150ul of β-1,4-glucosidase into the recombinant strain of Clostridium thermocellum DSM 1313 cellulite into 4ml of anaerobic fermentation medium, activate at 50°C After 18 hours, it was directly used as a saccharification strain after reactivation.

[0035] (2) Straw saccharification: transfer the pretreated straw to the anaerobic fermentation system, add medium according to the solid-to-liquid mass ratio of 1:12, add an appropriate amount of the mutagenized strain obtained in step (1) into the anaerobic fermentation tank, The straw saccharification was carried out at a temperature of 53° C. to obtain a saccharification solution mainly composed of glucose, and the concentration of glucose measured by high performance liquid chromatography reached 6...

Embodiment 3

[0039] Different from Example 1, a method for cultivating Chlorella pyrenoidosa with low cost, high biomass and high protein content comprises the following steps:

[0040] (1) Activation of Clostridium thermocellum strain: Inoculate 120ul of β-1,4-glucosidase into the recombinant strain of Clostridium thermocellum DSM 1313 cellulite into 4ml of anaerobic fermentation medium, activate at 50°C After 18 hours, it was directly used as a saccharification strain after reactivation.

[0041] (2) Straw saccharification: transfer the pretreated straw to the anaerobic fermentation system, add medium according to the solid-to-liquid mass ratio of 1:14, add an appropriate amount of the mutagenized strain obtained in step (1) into the anaerobic fermentation tank, Saccharification of straw was carried out at a temperature of 55°C to obtain a saccharification solution mainly composed of glucose, and the concentration of glucose measured by high performance liquid chromatography reached 64g / ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for cultivating Chlorella pyrenoidosa with low cost, high biomass and high protein content. The specific method adopts the method of carrying out straw saccharification of recombinant strains and using straw saccharification liquid as a cheap raw material to concurrently cultivate Chlorella . The method comprises the following steps: (1) activation of the recombinant strain of Clostridium thermocellum; (2) saccharification of straw: transferring the pretreated straw to an anaerobic fermentation system, adding medium and recombinant strains, and performing saccharification of straw; (3) ) preparation of chlorella pyrenoidosa culture medium; (4) concurrently cultivating chlorella pyrenoidosa: inserting appropriate amount of algae species into the culture medium and carrying out concurrent cultivation under appropriate light intensity. The present invention further improves the ability of Clostridium thermocellum to degrade lignocellulose to produce glucose through the recombinant strain of Clostridium thermocellum; and the obtained Chlorella pyrenoidosa has high biomass and high protein content, thus reducing the The production cost of chlorella improves the market competitiveness of chlorella pyrenoidosa and its derivative products.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a recombinant strain that integrates β-1,4-glucosidase into the cellulosome of Clostridium thermocellum DSM 1313 to carry out straw saccharification, and uses straw saccharification liquid as a cheap raw material for simultaneous cultivation Chlorella approach. Background technique [0002] The output of crop straw is huge, and the annual output of Shandong Province alone is as high as more than 70 million tons. Among them, about 15 million tons of straw are directly burned without utilization, which not only pollutes the environment, but also causes a great waste of cellulose resources. The main component of straw is cellulose, which can be hydrolyzed with cellulase to produce a high-concentration sugar solution, which can be used as a cheap sugar source for microalgae cultivation. [0003] Chlorella pyrenoidosa is recognized as "green gold" in the world. It is a high-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/21C12P19/14C12P19/02C12R1/89C12R1/145
CPCC12N1/12C12N1/20C12N9/2445C12P19/14C12P19/02C12Y302/01021
Inventor 崔球肖艳颜飞祁宽
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com