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Cryoprotectant and/or cryopreservant composition, methods and uses thereof

A cryoprotectant and composition technology, applied in the application, preservation of human or animal body, animal husbandry, etc., can solve the problems of chemical toxicity damage, low water chemical potential of high-concentration cryoprotectant, etc.

Inactive Publication Date: 2020-06-26
UNIV NOVA DE LISBOA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition to osmotic stress, the high concentrations of cryoprotectants required during vitrification can cause damage due to low water chemical potential or chemical toxicity 4

Method used

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  • Cryoprotectant and/or cryopreservant composition, methods and uses thereof
  • Cryoprotectant and/or cryopreservant composition, methods and uses thereof
  • Cryoprotectant and/or cryopreservant composition, methods and uses thereof

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Embodiment Construction

[0058] In one embodiment, the natural deep eutectic solvent (NADES) comprises trehalose-glycerol (molar ratio, 1:30; D(+) trehalose dihydrate, 99%, Sigma-Aldrich, glycerol, 99.9%, Sigma -Aldrich), prepared by heating at 70°C with stirring until a clear liquid forms.

[0059] In one embodiment, optical characterization of NADES samples is acquired by polarized light microscopy (POM) at room temperature using an Olympus transmission microscope coupled to a Leica digital camera. Covers were prepared with 1-2 drops of NADES and observed under a microscope.

[0060] In one embodiment, Differential Scanning Calorimetry (DSC) experiments are performed as follows: Calorimetric experiments are performed in a TA Instruments Inc. DSC Q2000 (Tzero DSC Technology) operating with the heat flow T4P option. Under anhydrous high-purity nitrogen at 50mL min -1 The flow velocity is measured. DSC Tzero calibration is carried out in the temperature range of -90 to 200°C. Pure compound water, t...

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Abstract

The present disclosure relates to cryoprotectant and / or cryopreservant composition, methods and uses thereof. This composition may be used in the preservation and / or protection of cell, organism and / or tissue.

Description

technical field [0001] The present disclosure relates to cryoprotectants and / or cryoprotectant compositions and methods and uses thereof. The composition can be used for the preservation and / or protection of cells, organisms and / or tissues. Background technique [0002] During the freezing process of cells, two main events occur. At ambient pressure and when cooled below 0°C at a rapid cooling rate, water forms ice in the form of hexagonal or cubic crystals. The formation of ice crystals can damage cell membranes and organelles, leading to cell death. Slow cooling reduces crystal formation but increases cell dehydration and shrinkage due to water penetration into the extracellular medium, which can also lead to physical stress on cell membranes and organelles, leading to cell death. In both cases, increased intracellular solute concentrations also lead to cell death. An optimal cooling rate can be found to be slow enough to prevent intracellular ice crystal formation, ye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 阿纳·雷塔·克鲁兹·杜阿尔特亚历山大·巴布·德·阿尔梅达·派瓦鲁伊·L·雷斯里塔·波拉·派瓦·克拉维罗若阿纳·马里亚·马克斯·达·席尔瓦瓦尼亚·伊萨贝尔·巴普蒂丝塔·德·卡斯特罗
Owner UNIV NOVA DE LISBOA
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