Eucommiaulmoides Oliver fermentation extract and preparation method thereof and application in cosmetics thereof

Abstract

The invention relates to a preparation method of Eucommiaulmoides Oliver fermentation extract, the Eucommiaulmoides Oliver fermentation extract obtained by the method, and a use of the Eucommiaulmoides Oliver fermentation extract for the preparation of cosmetics. The preparation method includes the following steps that a step of preparation of a composite strain is carried out, in the step, the composite strain is obtained by pre-culture of Acetobacter pasteurium, Lactobacillus plantarum and Saccharomyces cerevisiae separately; a step of enzymatic hydrolysis of Eucommiaulmoides Oliver powder to prepare a fermentation matrix is carried out, in the step, leaves of Eucommiaulmoides Oliver are washed, dried, crushed into powder and mixed with water, then Eucommiaulmoides Oliver enzymatic hydrolysate after enzymatic hydrolysis is obtained by adding protease, cellulase and pectinase for enzymatic hydrolysis, and the fermentation matrix is obtained based on the Eucommiaulmoides Oliver enzymatic hydrolysate; a step of fermentation is carried out, in the step, the composite strain is inoculated into the fermentation matrix containing the Eucommiaulmoides Oliver enzymatic hydrolysate after enzymatic hydrolysis and fermented under the condition of low dissolved oxygen to obtain fermentation broth; and a step of purification is carried out, in the step, the obtained fermentation broth is centrifuged and filtered to obtain the Eucommiaulmoides Oliver fermentation extract.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to the preparation of eucommia ulmoides fermentation extract and its application in cosmetics. Background technique [0002] In my country, plant-based traditional Chinese medicine has been used in natural skin care products for thousands of years. Compared with chemical synthesis, plant-based traditional Chinese medicine has the characteristics of better efficacy, less side effects, and higher safety. Therefore, in recent years, pure natural plant ingredients Skin care products based on cosmetics are becoming more and more popular among consumers. Eucommia ulmoides (Eucommiaulmoides Oliver) is a rare and precious Chinese medicinal material unique to my country. It has a medicinal history of more than 2,000 years and is called a "living fossil plant". It is distributed in more than 20 provinces across the country. hectares, accounting for more than 99% of the world's t...

AI Technical Summary

Problems solved by technology

Above method shortcoming mainly lies in: (1) physical or chemical extraction method, the leaching rate of active ingredient is not high, and there is the residue of organic reagents such as acetone, ethyl acetate in the extraction process, may be harmful to skin and human body, simultaneously to The environment may cause pollution; (2) There are more than 80 kinds of active ingredients in Eucommia, mainly flavonoids, polysaccharides, lignins, phenylpropanoids, etc., physical or chemical extraction methods, only one development and utilization of Eucommia raw materials, Active ingredients can not be effectively used, resulting in waste of traditional Chinese medicine resources

[0004] Chinese patent CN107927497A discloses "a production method of Eucommia fermented beverage", which is characterized in that the Eucommia fermented beverage is obtained by extracting Eucommia leaves, enzymatic hydrolysis, primary fermentation with lactic acid bacteria, and secondary fermentation with yeast at 4°C-6°C. The main disadvantages are: the optimum growth temperature of yeast is 25°C-30°C, and the fermentation at 4°C-6°C is not conducive to the growth and metabolism of yeast and the catalysis of enzymes, which is not conducive to the biotransformation of active ingredients in Eucommia leaves

The sugar content of this fermented beverage is 7%-20%, and contains a large amount of lactic acid bacteria and live yeast. The obtained fermented liquid has not undergone subsequent treatment, and its products are mainly used in the field of food and health products, and it is difficult to apply them as cosmetic raw materials in formulations , if used directly, it may destroy the micro-ecological balance system on the skin surface

[0005] Chinese patent CN106010926A discloses "A Eucommia Fermented Health Vinegar and Its Preparation Method", which is characterized in that firstly the extract of Eucommia is obtained by enzymolysis and ethanol extraction, and then the fruit wine is obtained by the primary fermentation of yeast and the secondary fermentation of acetic acid bacteria. The main disadvantages of eucommia fermented health vinegar are: long fermentation period, low production efficiency, and a large amount of live bacteria, acetic acid and alcohols in the product, which are mainly used in the field of food health products and cannot be directly used as cosmetic raw materials. , easily irritating to the skin

[0006] Chinese patent CN109498744A discloses "a preparation method of compound Eucommia leaf powder fermented by compound strains", which is characterized in that it contains astragalus, Codonopsis pilosula, Polygonatum, honeysuckle, Eucommia leaf obtained a kind of traditional Chinese medicine micro-ecological preparation: compound Eucommia leaf powder, its main disadvantages are: the long period of solid fermentation leads to low production efficiency, it is easy to be contaminated with miscellaneous bacteria during the cultivation process, and the fermentation bacteria are not probiotics , the culture medium components are expensive, mainly used in the field of feed, and cannot be directly used as cosmetic raw materials

[0007] Chinese patent CN108721367A discloses "a compound fermentation bacterium used in the preparation of Eucommia decoction pieces", which is characterized in that the Eucommia bark slices are compositely fermented by Aspergillus oryzae, Bacillus natto and Candida utilis, and dried at low temperature to obtain Eucommia decoction pieces , the main disadvantages are: the fermented bacteria are not probiotics, and although the content of chlorogenic acid and pinoresinol diglucoside in Eucommia slices has been increased through fermentation, the active ingredients are difficult to release into the fermentation broth due to the fermentation of Eucommia slices , mainly used in the field of food and health products, and cannot be directly used as cosmetic raw materials

[0009] 1. Eucommia ulmoides fermentation is mostly used in the field of food and health products, and there is no patent report on the application of its fermentation extract in the field of cosmetics

[0010] 2. The application of Eucommia ulmoides as raw materials in the field of cosmetics is mainly to obtain eucommia ulmoides extracts through ordinary extraction methods, and there are problems such as low extraction rate of active ingredients, residual organic reagents, and simple development and utilization of eucommia ulmoides

[0011] 3. The patented technology using Eucommia fermented liquid as a product is mainly to ferment one or more probiotics in sequence, and does not make full use of the complementary and synergistic advantages of different strains, thus causing a waste of Eucommia resources

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