Nucleic acid extraction method for gene sequencing

Abstract

The invention discloses a nucleic acid extraction method for gene sequencing. The method concretely comprises the following steps: S1, respectively adding a cell lysing solution and magnetic beads into two different first test tubes which are marked as a test tube I and a test tube II, adding a sample to be extracted into the cell lysing solution, controlling a nucleic acid extraction device to rotate stirring paddles to fully lyse cells in the sample; S2, controlling the nucleic acid extraction device to transfer the magnetic beads into the test tube I through an electromagnet, and then controlling the stirring paddles to stir the cell liquid to enable the magnetic beads to be fully combined with nucleic acid; and S3, attracting the magnetic beads out of the test tube I, transferring themagnetic beads to the test tube II, adding an eluent into the test tube II, controlling stirring paddles to fully separate the nucleic acid from the magnetic beads, and taking out the magnetic beads from the test tube II to obtain the required nucleic acid. According to the invention, nucleic acid is extracted by a magnetic bead method, and other impurity components in the sample can be removed through the steps of lysing precipitation, magnetic attraction and elution, so that the nucleic acid can be automatically and efficiently extracted.

Description

technical field [0001] The invention relates to the technical field of gene sequencing, in particular to a nucleic acid extraction method for gene sequencing. Background technique [0002] Gene sequencing is one of the methods of genetic testing, also known as gene spectrum sequencing, which is an internationally recognized standard for genetic testing. The first step in gene sequencing requires the extraction of nucleic acid from the sample. The nano-magnetic beads use superparamagnetic nano-microspheres as the carrier, which can quickly separate and purify DNA or RNA, and can be automated, safe and fast, and can reduce the impact of organic solvents. Injury to the human body, the nucleic acid that can be used to extract the target sample will help people conduct corresponding experimental analysis and make corresponding judgments. [0003] The magnetic bead method nucleic acid extraction is based on the same principle as the silica gel membrane spin column, and the surfac...

AI Technical Summary

Problems solved by technology

[0005] 1. By using the magnetic bead method for nucleic acid extraction, the problems of complex operation and low extraction efficiency of existing nucleic acid extraction methods are solved;

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