Oil seal device for digital PCR chip

An oil seal and chip technology, which is applied in the field of oil seal devices for digital PCR chips, can solve the problems of long operation time, error possibility, and many steps, and achieve the effects of ensuring effectiveness, reducing errors, and ensuring stability

Active Publication Date: 2020-06-19
宁波胤瑞生物医学仪器有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The main disadvantages of the above operation are that there are many steps in the operation process, the process is complicated, there are certain technical requirements for the operator, the possibility of human-induced errors is relatively high, the operation time is long, and the mixed liquid in the reaction tank of the micro system may be volatile and cause results. inaccurate

Method used

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  • Oil seal device for digital PCR chip
  • Oil seal device for digital PCR chip
  • Oil seal device for digital PCR chip

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Embodiment Construction

[0026] The preferred embodiments of the invention are described below with reference to the drawings. Those skilled in the art should understand that these embodiments are only used to explain the technical principles of the invention and are not intended to limit the protection scope of the invention.

[0027] It should be noted that in the description of the invention, the terms "upper", "lower", "left", "right", "inner", "outer" and other terms indicating the direction or positional relationship are based on the attached drawings. The direction or positional relationship of is only for ease of description, and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the invention.

[0028] In addition, it should be noted that, in the description of the invention, unless otherwise clearly specified and limited, the terms "installation", "conn...

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Abstract

The invention provides an oil seal device for a digital PCR chip. The device comprises a base, the base is made of an aluminum material, a groove is formed in the middle part of the base, the groove is used as a container for placing a reaction chip, an upper cover is arranged on the groove, the upper cover slides relative to the substrate to seal the chip, and paraffin oil with a thickness of 0.3-0.5 mm is used as an oil seal structure at the position, corresponding to the chip, of the upper cover; and sliding grooves are formed in the two opposite sides of the inner side of the groove, the upper cover can horizontally slide in the sliding grooves, and the upper side faces of the side walls of the sliding grooves are oil sealing faces. Due to full-closed and semi-automatic operation of the device, the manual operation process is reduced, and therefore errors caused by manual operation can be reduced to the maximum extent; a good heat-conducting medium is selected, so that the heat conductivity of the device as a reaction chip container is fully guaranteed, and the stability of reaction conditions is fully guaranteed; and a material and a structure adopted by the upper cover surface can fully guarantee effectiveness of later result detection.

Description

Technical field [0001] The present invention relates to the technical field of oil seals for nucleic acid amplifiers, and in particular to an oil seal device for digital PCR chips. Background technique [0002] The application of DNA molecular amplification technology has promoted the development of molecular biology technology, and accurate quantification of DNA copy number is one of the important applications of modern molecular biology and medicine. In 1997, James F. Brown, Jonathan E. Silver and Olga V. Kalinina constructed the prototype of digital PCR (digital polymerase chain reaction, dPCR) technology and obtained a US patent (patent number: US6143496A). Different from traditional PCR technology, digital PCR fully dilutes nucleic acid samples so that the number of sample templates in each reaction chamber is less than or equal to one. After PCR amplification is completed, the fluorescent signal of each reaction chamber is statistically analyzed to solve Real-time quantita...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00
CPCC12Q1/6851C12Q2531/113C12Q2565/607
Inventor 任鲁风蔡亦梅金鑫浩李洁昆张未来于军
Owner 宁波胤瑞生物医学仪器有限责任公司
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