Oil seal device for digital PCR chip

Abstract

The invention provides an oil seal device for a digital PCR chip, which comprises a substrate, wherein the substrate is made of an aluminum material, a groove is formed in the middle part of the substrate, the groove is used as a container for placing a reaction chip, an upper cover is arranged on the groove, the upper cover slides relative to the substrate to seal a chip, and paraffin oil with athickness of 0.3-0.5 mm is used as an oil seal structure at the position, corresponding to the chip, of the upper cover; a plurality of springs are arranged at the upper end of the inner side wall ofthe groove, one end of each spring is connected with the upper cover, and the side face of the groove in the upper side of the upper cover after the upper cover is buckled is an oil sealing face. By means of full-closed and semi-automatic operation, the manual operation process is reduced, and therefore errors caused by manual operation can be reduced to the maximum extent; a good heat-conductingmedium is selected, so that the heat conductivity of the device as a reaction chip container is fully guaranteed, and the stability of reaction conditions is fully guaranteed; the upper cover surfaceis made of the materials and of a structure that can fully guarantee later result detection effectiveness.

Description

technical field [0001] The invention relates to the technical field of oil seals for nucleic acid amplification instruments, in particular to an oil seal device for digital PCR chips. Background technique [0002] The application of DNA molecular amplification technology has promoted the development of molecular biology technology, and the precise quantification of DNA copy number is one of the important applications of modern molecular biology and medicine. In 1997, James F.Brown, Jonathan E.Silver and OlgaV.Kalinina built the prototype of digital PCR (digital polymerase chain reaction, dPCR) technology, and obtained the US patent (patent number: US6143496A). Different from traditional PCR technology, digital PCR fully dilutes nucleic acid samples so that the number of sample templates in each reaction chamber is less than or equal to 1. After PCR amplification, the fluorescent signals of each reaction chamber are statistically analyzed to solve the problem. A series of pr...

AI Technical Summary

Problems solved by technology

[0005] The main disadvantages of the above operation are that there are many steps in the operation process, the process is complicated, there are certain technical requirements for the operator, the possibility of human-induced errors is relatively high, the operation time is long, and the mixed liquid in the reaction tank of the micro system may be volatile and cause results. inaccurate

Images