Extraction and purification device and method utilizing magnetic extraction material

Abstract

The invention relates to an extraction and purification device and method. The extraction and purification device comprises at least one pipetting module, at least five station modules, a waste liquidbox, a waste box and a first guide rail; the pipetting module or the station module can move on the first guide rail, the station module is arranged below the pipetting module and comprises at leastone pipetting head station module, at least one extraction station module and at least three reagent station modules, and the station module, the waste liquid box and the waste box are arranged in themoving direction of a first guide rail; an extraction material is arranged in the extraction station module, the extraction station module comprises a stirrer and a power supply, and the power supplycontrols the stirrer to attract, fix and stir an extraction material; the pipetting module comprises a liquid driving device and a second guide rail, and the liquid driving device can move up and down on the second guide rail. The volume of the extraction material is not less than 1 cubic millimeter, and the surface of the extraction material has a mesoporous structure and has adsorption or exchange capacity.

Description

technical field [0001] The invention belongs to the field of in vitro clinical testing sample processing, and in particular relates to an extraction and purification device and method. Background technique [0002] In the field of clinical testing and precision medicine, instruments such as mass spectrometry, chromatography, or liquid chromatography-mass spectrometry have advantages in sensitivity, versatility, analysis speed, and simultaneous detection of multiple indicators. These high-precision analytical instruments can be used in clinical biochemistry, clinical Immunological testing, clinical microbiological testing, proteomics, metabolomics research and other fields. However, high-precision analytical instruments have relatively strict requirements on the pretreatment of test samples. In addition, due to the complexity of biological test samples, the difficulty of sample pretreatment is relatively high, and there are still the following problems to be solved: (1) Sampl...

AI Technical Summary

Problems solved by technology

However, high-precision analytical instruments have relatively strict requirements on the pretreatment of test samples. In addition, due to the complexity of biological test samples, the difficulty of sample pretreatment is relatively high, and there are still the following problems to be solved: (1) Sample pretreatment steps Numerous, complex processes, high requirements for personnel skills; (2) non-intelligent, large human interference factors, easy to cause unstable results, large errors, and high error rates; (3) long experiment cycle, or low processing efficiency, cost high

However, the disadvantages of this method are: 1) it takes a long time for the target substance in the liquid sample to reach adsorption equilibrium in the extraction material and the solution; 2) the process of separating the solid phase from the solution is more complicated

[0005] Most of the previous devices used the XYZ axis type, and the processing speed was slow, so that the mass spectrometer was always in a state of waiting for samples. Although the processing speed was improved to a certain extent through simultaneous processing of multiple channels, the mass spectrometer still had a long interval

Taking the processing of 96 samples as an example, the pre-processing instrument cannot be sent to the mass spectrometer immediately after processing, and it needs to wait for 96 samples to be processed at the same time, while the mass spectrometer detects one by one, resulting in the interval between the first sample and the last sample very long

Cannot meet the needs of current clinical testing

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