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Application of human umbilical cord mesenchymal stem cell exosome

A technology of mesenchymal stem cells and human umbilical cord, applied in the field of drug therapy, can solve the problems of inability to achieve treatment, loss of function, destruction of pancreatic β-cells, etc.

Pending Publication Date: 2020-06-16
博生众康(厦门)医药生物技术股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the therapeutic purpose of these drugs is to lower blood sugar, they can only achieve the purpose of symptomatic treatment, but cannot achieve the purpose of treating the cause.
Thus, as the disease progresses, it leads to progressive destruction of pancreatic β-cells, eventually leading to their loss of function

Method used

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  • Application of human umbilical cord mesenchymal stem cell exosome
  • Application of human umbilical cord mesenchymal stem cell exosome
  • Application of human umbilical cord mesenchymal stem cell exosome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Cell culture and hucMSCs-Exo isolation and identification

[0023] 1. Separation:

[0024] Human umbilical cord mesenchymal stem cells (hucMSCs) were cultured with DMEM / F-12 1:1 containing 10% fetal bovine serum (referring to the 1:1 volume ratio of DMEM containing 10% fetal bovine serum and F-12) medium , the cells were isolated from neonatal umbilical cord. The day before induction of differentiation, hucMSCs cells were inoculated into 10 cm culture plates, so that the confluence of the cells on the second day was 50-70%, rinsed three times with 1xPBS, and replaced with serum-free DMEM / F-12 1:1 (referring to serum-free The volume ratio of DMEM to F-12 is 1:1) medium, continue to cultivate until 48 hours to collect the supernatant, add serum-free medium again, and collect the culture supernatant again after 48 hours. The collected cell culture supernatant was 4°C, 3000rpm, 15 minutes, 4°C, 10000xg, 30 minutes, passed through a 0.22um filter membrane, centr...

Embodiment 2

[0049] Example 2: In vitro functional experiment of hucMSCs-Exo

[0050] 1. Establishment of mouse type 1 diabetes model

[0051] (1) Purchase 8-week-old C57BL / 6J male mice, 12 hours after fasting.

[0052](2) Protect from light Weigh an appropriate amount of streptozotocin (STZ) and dissolve it in citric acid buffer to prepare a 10 mg / mL STZ solution, which is prepared and used immediately. The mice were weighed, and the mice in the model group were injected intraperitoneally with 60 mg / kg STZ for 5 consecutive days, and the mice in the control group were injected intraperitoneally with a corresponding volume of citrate buffer. Since the STZ aqueous solution is extremely unstable and easy to decompose when exposed to light, the whole process should be carried out in the dark and completed within 30 minutes. Feed 1 hour after administration.

[0053] (3) Three days later, the blood glucose of the mice was measured. If the blood glucose of the mice was greater than 16.7mM, t...

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Abstract

The invention discloses an application of human umbilical cord mesenchymal stem cell exosome. Specifically, the invention relates to application of human umbilical cord mesenchymal stem cell exosome in preparation of drugs for treating diabetes. CK19 positive pancreatic duct epithelial cells can be promoted to be differentiated into pancreatic beta cells capable of secreting insulin, so that the quantity and functions of beta-cells are increased, and a relatively good blood glucose regulation effect is achieved on type 1 diabetes mice.

Description

technical field [0001] The invention relates to the field of drug therapy, in particular to the use of human umbilical cord mesenchymal stem cell exosomes. Background technique [0002] Whether it is type 1 or type 2 diabetes, as its pathology progresses, there will be progressive destruction of the structure and function of β-cells, which will eventually lead to absolute deficiency of insulin secretion, which will lead to various diabetic complications, and finally endanger the lives of patients. [0003] At present, the treatment of diabetes mainly includes the following methods: insulin and its analogues, insulin sensitizers, insulin secretagogues and some new hypoglycemic drugs. But because the treatment purpose of these medicines is hypoglycemic, can only play the purpose of symptomatic treatment, but can't reach the purpose of cause treatment. Therefore, as the disease progresses, it will lead to progressive destruction of pancreatic β-cells, eventually leading to the...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P3/10A61P5/48
CPCA61K35/28A61P3/10A61P5/48
Inventor 李良成钱丽霞黄飞榕
Owner 博生众康(厦门)医药生物技术股份有限公司
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