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Method for preventing and treating spodoptera frugiperda through tetrastichus howardi

A technology for Spodoptera frugiperda and Spodoptera frugiperda is applied in the field of biological control of agricultural pests and Spodoptera frugiperda. High cost and the effect of increasing the parasitic rate

Inactive Publication Date: 2020-06-16
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a method for controlling Spodoptera frugiperda by using the hordei to overcome the above-mentioned deficiencies of high multiplication cost and poor control effect of the existing parasitic wasps; and further overcome the 3R problem in chemical control

Method used

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  • Method for preventing and treating spodoptera frugiperda through tetrastichus howardi
  • Method for preventing and treating spodoptera frugiperda through tetrastichus howardi
  • Method for preventing and treating spodoptera frugiperda through tetrastichus howardi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Expansion of the natural enemy of Spodoptera frugiperda, H.

[0025] (1) The inventor found a parasitic wasp - Tetrastichushowardi Olliff (Tetrastichushowardi Olliff) in the area where Spodoptera frugiperda occurs. The parasitic wasp parasitizes the pupae of Spodoptera frugiperda and has strong fecundity. It finds a high-quality parasitic natural enemy for controlling Spodoptera frugiperda.

[0026] (2) The honeycomb room has the conditions of heat preservation, moisture retention, ventilation, light transmission, insect protection, and rodent protection, and is equipped with temperature, humidity, and lighting equipment. Keep the temperature at 24°C-28°C, relative humidity at 45%-65%, and light for 12 hours. The walls and floors of the beehives should be easy to clean and disinfect and should be kept clean and hygienic. Place the insect rearing box in the breeding room, and carry out disinfection and sterilization to the breeding room and the insect raisin...

Embodiment 2

[0034] Example 2. Preparation of Beauveria bassiana powder.

[0035] (1) The acquisition of Beauveria bassiana.

[0036] In the cornfield of Danzhou, Hainan, the dead pupae of Spodoptera frugiperda parasitized by Beauveria bassiana were found. The pathogen was isolated according to the conventional method, and it was identified as Beauveria bassiana, and then the isolate was tested for pathogenicity, and the highly pathogenic strain D-1 was screened out. A large amount of spore powder of Beauveria bassiana coccidioides D-1 was obtained by solid-state culture method.

[0037] (2) Beauveria bassiana powder.

[0038] Beauveria bassiana powder is a mixture of Beauveria bassiana spore powder, defatted corn flour, dextrin and soybean lecithin. Defatted cornmeal, which is harmless to parasitoids, was chosen as powder carrier; dextrin was used as binder; soybean lecithin was used as wetting agent. The mixing ratio is 16:2:1:1, and the effective content of Beauveria bassiana powder...

Embodiment 3

[0039] Example 3. Release of Bacteria chrysalis.

[0040] (1) Beauveria bassiana powder carried by H.

[0041] Spray coccidioides beauveria bassiana powder to the newly hatched wasps with a powder sprayer, every 500 spray powder 2g.

[0042] (2) Determination of the number of bees released

[0043] Through sampling survey, the field population of fall armyworm was estimated. The control effect is the best when the ratio of the released total amount of H. frugiperi to the estimated field population of Spodoptera frugiperda is 4:1.

[0044] (2) The method of releasing bees

[0045] The beauveria bassiana carrying Beauveria bassiana should be released in the field within 1-2 days. Released every 2 weeks for 3 consecutive releases. The reduction rate of insect population will reach more than 90%, and the crop yield will recover obviously. The wasp is like a biological missile, which can carry Beauveria bassiana to accurately find the pupae of Spodoptera frugiperda in the soil....

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Abstract

The invention relates to a parasitic natural enemy tetrastichus howardi of an invasive pest spodoptera frugiperda. The parasitic natural enemy tetrastichus howardi can effectively prevent and treat spodoptera frugiperda. A method comprises the following steps of performing propagation expanding on the parasitic natural enemy tetrastichus howardi, wherein initial pupae of yellow mealworms are usedas substitute hosts; performing spraying treatment on the initial pupae of the yellow mealworms for propagation expanding with an ethanol diluent (1000 times) of trans-beta-farnesene; and before release of adult tetrastichus howardi; and performing powder spraying with beauveria bassiana (D-1) powder (beauveria bassiana spore powder, dextrin, defatted corn flour and soybean lecithin), wherein theparasitic natural enemy tetrastichus howardi can carry beauveria bassiana to be accurately parasitic on the target pest spodoptera frugiperda in a directional manner, and a biocontrol substance can becolonized and can continuously control the pests. According to the technology, the propagation expanding cost is effectively reduced, and the parasitic efficacy, the stability and the persistence areimproved.

Description

technical field [0001] The invention relates to a method for controlling Spodoptera frugiperda Smith by using Tetrastichus howardi Olliff. The invention belongs to the technical field of plant protection, more specifically the technology of biological control of agricultural pests. Background technique [0002] Spodoptera frugiperda Smith, belonging to the Lepidoptera order Lepidoptera, is native to tropical and subtropical America and is widely distributed in the American continent. It has invaded 46 countries and regions in Africa, including 9 countries including China. An Asian country, it is a major transboundary migratory pest on food crops such as sugar cane, sorghum and corn. The insect was confirmed to have invaded Yunnan Province of my country in January 2019 and has spread to some urban areas in South China, Southwest China, Central China and East China. . In order to prevent the spread of the newly invaded pest Spodoptera frugiperda, chemical agents are mainly use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 吕宝乾卢辉唐继洪苏豪何杏陈运雷
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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