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A kind of phage composition and its application in preventing and treating chicken oviduct inflammation

A technology of salpingitis and bacteriophage, applied in the field of microorganisms, can solve the problems of long treatment time, unsatisfactory effect, and high cost of medication, and achieve the effects of a large number, broadening the scope of sterilization, and wide application

Active Publication Date: 2021-08-31
QINGDAO PHAGEPHARM BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional Chinese medicine has some shortcomings such as long treatment time, unsatisfactory effect, and high cost of medication.
At present, there is no safe and effective drug for the prevention and treatment of chicken salpingitis

Method used

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  • A kind of phage composition and its application in preventing and treating chicken oviduct inflammation
  • A kind of phage composition and its application in preventing and treating chicken oviduct inflammation
  • A kind of phage composition and its application in preventing and treating chicken oviduct inflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The isolation and preparation of embodiment 1 phage

[0042] The fecal liquid sewage, chicken intestinal tract and liver samples in the present invention are collected from many chicken farms in Shandong Province.

[0043] 1. Isolation and identification of pathogenic bacteria

[0044] From a laying hen farm in Shandong, sick chickens with salpingitis were collected for dissection, and pathogenic bacteria were isolated from the oviducts. They were cultured in SS medium, and the pathogenic bacteria obtained were identified by microscopic examination and identified by corresponding PCR.

[0045] Results: On the SS medium, small off-white colonies with black hearts were isolated, with smooth and raised colony edges, which were identified as Salmonella by PCR with specific primers for Salmonella; red colonies with a slightly lighter color around them were also isolated on the SS medium, and colonies were tested by large intestine Escherichia coli was identified by PCR wit...

Embodiment 2

[0051] Morphological characteristics of embodiment 2 coli phage PD07 and Salmonella phage PYC04 Bacteriophage PD07 is observed under transmission electron microscope (see figure 1 ). The head of the phage has a polyhedral structure and has a slender tail. The long diameter of the phage head is about 86.67 nm, the transverse diameter is about 106.67 nm, and the tail is about 120 nm. According to the definition of the International Committee on Taxonomy of Viruses (ICTV), the morphology of bacteriophage PD07 conforms to the characteristics of Myoviridae and belongs to Myoviridae.

[0052] Phage PYC04 was placed under a transmission electron microscope for observation (see figure 2 ). The head of the phage has a polyhedral structure and has a slender tail. The long diameter of the phage head is about 66.67nm, the transverse diameter is about 60nm, and the tail is about 133.33nm. According to the definition of the International Committee on Taxonomy of Viruses (ICTV), the morp...

Embodiment 3

[0053] Whole Genome Analysis of Example 3 Phage PD07 and PYC04

[0054] The genomes of Escherichia coli phage PD07 and Salmonella phage PYC04 were respectively extracted and sequenced to obtain:

[0055] The genome size of phage PD07 is 40629bp, and phage PD07 is a double-stranded DNA virus. The proportions of the four bases of A, G, C, and T in the whole genome sequence are 23.81%, 23.38%, 26.51%, 26.38%, respectively, and G+ The C% content is 49.89%. The genome size of bacteriophage PYC04 is 41843bp, which is a circular double-stranded DNA. The proportions of the four bases of genome sequence A, G, C, and T are 25.03%, 24.94%, 24.56%, and 25.47%, respectively, and the content of G+C% is 49.50%.

[0056] According to RAST prediction analysis, the genome of phage PD07 contains 271 open reading frames (ORFs), and the genome of phage PYC04 contains 62 open reading frames (ORFs). Compared with 62 ORFs protein sequences of phage PYC04, phage PD07 contained 24 known encoded func...

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Abstract

The invention discloses a phage composition and its application in preventing and treating chicken oviductitis. The phage composition includes coli phage PD07 and Salmonella phage PYC04; the coli phage PD07 has a preservation number of CGMCC No18865 and was released in 2019. It was deposited in the China General Microorganism Culture Collection and Management Center on November 4; the Salmonella phage PYC04, with the preservation number CGMCC No18867, was preserved in the China General Microbiology Culture Collection and Management Center on November 4, 2019. The phage composition is used as an effective component for preventing and treating chicken salpingitis through the compounding of coliphage PD07 and Salmonella phage PYC04. Compared with a single lytic coliphage, it greatly broadens its bactericidal range and increases its bactericidal activity , can increase the laying rate of laying hens, enhance the physique and disease resistance of laying hens.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacteriophage and its application in medicine for preventing and treating chicken oviductitis. Background technique [0002] The common cause of chicken salpingitis is due to biological pathogenic bacteria infection, Escherichia coli and Salmonella are the most common pathogens. A white pus-like inflammatory exudate is common from the fallopian tubes, and the feathers below the anus are often contaminated by inflammatory substances. When the fallopian tubes are inflamed, the eggshells will become thinner, and the proportion of eggs with sandy shells, deformed eggs, and eggs with broken shells will increase, and the appearance of eggs will deteriorate. Salpingitis affects the quality of eggs, and in severe cases, inflammation spread and systemic infection can occur, causing chicken death and causing significant economic losses. [0003] In my country, in order to ensure...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12Q1/70A61K35/76A61P15/00A61P31/14A01N63/40A01P1/00C12R1/92
CPCC12N7/00A61K35/76C12N2795/10121C12Q1/70A61P15/08A61P31/04A61P1/12A01N63/40A23K10/18A23K50/75C12N2795/10131C12N2795/10133C12N2795/10321C12N2795/10331C12N2795/10333A61K2300/00Y02A50/30
Inventor 潘强任慧英孙虎芝刘广芹葛超罗志祥
Owner QINGDAO PHAGEPHARM BIO TECH CO LTD
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