Squid skin collagen and rose extract compound beverage and preparation method thereof
A technology of rose extract and skin collagen, applied in food ingredients containing natural extracts, fish protein components, food extraction, etc., can solve the problems of low resource utilization, no beauty effect, single function, etc., to achieve The effects of high resource utilization, low production cost, and simple preparation process
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Embodiment 1
[0038] (a) Preparation of iron-based squid skin collagen:
[0039] (1) The squid skin is thawed, after the tissue is mashed, it is immersed in 0.1moL / L NaOH solution and degreased twice, according to the ratio of squid skin mass (g):NaOH solution volume (mL)=1:5, soak and degrease two times. time, 2 hours each time; after soaking for 2 hours for the first time, wash with deionized water until the eluate is colorless, transparent and clear, then soak for the second time, after soaking for 2 hours, wash with deionized water until the eluate is free The color is transparent and clear, filtered and dried;
[0040] (2) Add iron dextran solution with a concentration of 0.05mol / L to the squid skin treated in step (1), adjust the mass ratio of solid to liquid to 1:15, adjust the pH to 7.5, raise the temperature to 35°C, and add trypsin , constant temperature enzymatic hydrolysis for 1.5h; adjust the pH to 2, add pepsin, constant temperature enzymatic hydrolysis for 1h, heat up to ina...
Embodiment 2
[0045] (a) Preparation of iron-based squid skin collagen:
[0046] (1) The squid skin is thawed, after the tissue is mashed, it is immersed in 0.5moL / L NaOH solution and degreased twice, according to the ratio of squid skin mass (g):NaOH solution volume (mL)=1:5, soak and degrease two times. time, 4 hours each time; after soaking for 4 hours for the first time, wash with deionized water until the eluate is colorless, transparent and clear, then soak for the second time, after soaking for 4 hours, wash with deionized water until the eluate is free The color is transparent and clear, filtered and dried;
[0047] (2) Add iron dextran solution with a concentration of 0.15mol / L to the squid skin treated in step (1), adjust the mass ratio of solid to liquid to 1:20, adjust the pH to 8.5, raise the temperature to 45°C, and add trypsin , constant temperature enzymatic hydrolysis for 1 h; adjust the pH to 3, add pepsin, constant temperature enzymatic hydrolysis for 0.5 h, heat up to i...
Embodiment 3
[0052] (a) Preparation of iron-based squid skin collagen:
[0053] (1) The squid skin is thawed, after the tissue is mashed, it is immersed in 0.3moL / L NaOH solution and degreased twice, according to the ratio of squid skin mass (g):NaOH solution volume (mL)=1:5, soak and degrease two times. time, 3 hours each time; after soaking for 3 hours for the first time, wash with deionized water until the eluate is colorless, transparent and clear, then soak for the second time, after soaking for 3 hours, wash with deionized water until the eluate is free The color is transparent and clear, filtered and dried;
[0054] (2) Add iron dextran solution with a concentration of 0.05-0.15mol / L to the squid skin after step (1), adjust the mass ratio of solid to liquid to 1:18, adjust the pH to 8.0, heat up to 40°C, add Trypsin, constant temperature enzymatic hydrolysis for 1.2h; adjust the pH to 2.5, add pepsin, constant temperature enzymatic hydrolysis for 0.8h, heat up to inactivate the enz...
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