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Purine alkali plant immune resistance inducer and application thereof

An immune inducer and plant technology, applied in plant growth regulators, plant growth regulators, applications, etc., can solve the problems of lack of effective fungicides for oomycetes, difficulty in preventing and controlling plant diseases, etc., to prevent large-scale diseases Occurrence, good control effect, good effect

Active Publication Date: 2020-06-12
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to control the diseases caused by this group of pathogenic organisms, mycologists and plant pathologists from all over the world have conducted a lot of research on Phytophthora from different angles, but due to the difficulty in obtaining long-lasting resistance sources from host plants and the lack of Effective fungicide against oomycetes, therefore, difficult to control plant diseases caused by oomycetes

Method used

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  • Purine alkali plant immune resistance inducer and application thereof
  • Purine alkali plant immune resistance inducer and application thereof
  • Purine alkali plant immune resistance inducer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Test crop: rice (Japonica rice Zhonghua 11).

[0043] 2. Planting method: Rice is planted in a greenhouse with 16 hours of light and 8 hours of darkness every day. The cultivation temperature is 26℃ and the air humidity is 60-70%. The greenhouse is located in the State Key Laboratory of Crop Biology, Shandong Agricultural University .

[0044] 3. Test method:

[0045] The plant immune inducer (containing 100ng / ml guanine solution and 0.05% Silwet L-77) was sprayed on the leaves and stems of rice cultivated for 8 weeks, and the mixture containing only 0.05% Silwet L-77 The aqueous solution is used as a control. Two hours later, the cultured sheath blight fungus was wrapped around the wooden match stick, and the wooden match stick with the fungus was embedded in the rice leaf sheath by the embedding method and sealed with a plastic wrap to keep it moisturizing. Four days after the inoculation, the diseased parts of the rice were cut off and the phenotype of the diseased sp...

Embodiment 2

[0050] 1. Test crop: rice (Japonica rice Zhonghua 11).

[0051] 2. Planting method: Rice is planted in a greenhouse with 16 hours of light and 8 hours of darkness every day. The cultivation temperature is 26℃ and the air humidity is 60-70%. The greenhouse is located in the State Key Laboratory of Crop Biology, Shandong Agricultural University .

[0052] 3. Experimental method:

[0053] When the rice is cultivated to the 6th week, spray the plant immune inducer (containing 100ng / ml guanine solution and 0.05% Silwet L-77) on the rice leaves, and use only 0.05% Silwet L-77. The aqueous solution was used as a control. After 2 hours, use 10mM MgCl 2 Elute the bacterial Xanthomonas oryzae RS105 cultured on the plate and adjust the OD value to 0.005. Use a sterile medical syringe to inoculate the rice leaves. After 10 days of inoculation, take a sample and take pictures of the diseased leaves to measure the disease. Spot length and the number of bacteria were measured.

[0054] 4. Test re...

Embodiment 3

[0057] 1. Test crop: rice (Japonica rice Zhonghua 11).

[0058] 2. Planting method: Rice is planted in a greenhouse with 16 hours of light and 8 hours of darkness every day. The cultivation temperature is 26℃ and the air humidity is 60-70%. The greenhouse is located in the State Key Laboratory of Crop Biology, Shandong Agricultural University .

[0059] 3. Test method:

[0060] Rice was sprayed with 0.05% Silwet L-77 aqueous solution (control) and 100ng / ml guanine (containing 0.05% Silwet L-77) for 2 hours, and then used 10mM MgCl 2 Elute the bacterial blight strain PXO99A cultured on the plate and adjust the OD value of the solution to 0.5. Use scissors with a matte surface to dip the bacteria solution to cut and inoculate the rice leaf tips. 18 days after inoculation, Take pictures of diseased leaves and measure the length of the lesion.

[0061] 4. Test results:

[0062] image 3 A and B respectively showed that the length of rice disease spot after guanine treatment was only 63% o...

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Abstract

The invention discloses a purine alkali plant immune resistance inducer and application thereof, and belongs to the technical field of biopesticides. It is found that guanine can improve the defense response of plants and reduce the morbidity and particularly has a good prevention and treatment effect on oomycete diseases of crops, and therefore guanine and structural analogues thereof can serve as effective components of the plant immune resistance inducer. The plant immune resistance inducer prepared with guanine or structural analogues thereof as effective components is mainly applied in aspraying method during application, is simple and convenient to operate, can be sprayed in advance in a high-incidence period of plants, and can effectively prevent large-area occurrence of diseases.

Description

Technical field [0001] The invention relates to the technical field of biological pesticides, in particular to a purine base plant immune inducer and its application. Background technique [0002] my country is a large agricultural country, and food is a necessity for the people, which is of great significance to the stable growth of the national economy. However, the current crop diseases are serious. Due to different diseases caused by pathogens, crops worth billions of dollars are lost every year. For a long time, the main method for farmers to prevent and control diseases is chemical prevention and control. By spraying pesticides, pathogens are used as direct targets to quickly, directly and comprehensively kill pathogens to achieve the purpose of disease control. However, due to problems such as large dosage of pesticides and unscientific application, it is easy to cause environmental pollution, crop phytotoxicity, and pathogenic bacteria produce drug resistance and other s...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01N25/30A01P21/00A01P1/00A01P3/00
CPCA01N25/30A01N43/90
Inventor 丁新华路冲冲李洋储昭辉王露露
Owner SHANDONG AGRICULTURAL UNIVERSITY
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