A kind of tissue engineered bone based on multilayer cell grid and its preparation method

A technology for tissue engineering bone and stratified cells, applied in tissue regeneration, additive processing, pharmaceutical formulations, etc., can solve problems such as poor mechanical properties and difficult to degrade, and achieve the effect of maintaining activity, high strength, and good mechanical properties

Active Publication Date: 2021-07-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its good biocompatibility and other characteristics, hydrogels are used to make cell scaffolds to repair bone defects, but traditional hydrogels limit their use due to their poor mechanical properties, water absorption swelling, and easy degradation. Therefore, it is of great significance to find a new type of hydrogel material with good mechanical properties, not easy to degrade, and good biocompatibility to construct a biological cell scaffold for repairing bone defects.

Method used

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  • A kind of tissue engineered bone based on multilayer cell grid and its preparation method
  • A kind of tissue engineered bone based on multilayer cell grid and its preparation method
  • A kind of tissue engineered bone based on multilayer cell grid and its preparation method

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Experimental program
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Effect test

Embodiment 1

[0041] 1. Preparation of Grid Scaffolds

[0042] (1) Preparation of printing materials

[0043]Sodium p-styrenesulfonate (NaSS, anionic monomer) and 3-(methacryloylamino)propyltrimethylammonium chloride (MPTC, cationic monomer) were added to 0.05mol% of 2-ketopentadiene Acid (photoinitiator), at 365nm wavelength, 7.5mW / cm2Ultraviolet light irradiation for 8 hours, prepared 1mol / L anion precursor aqueous solution and cationic precursor aqueous solution respectively. It was then precipitated by adding ethanol, and the precipitate was dried in an oven. PMPTC transparent particles with a weight average molecular weight of 4×10 3 g / mol and a polydispersity of 1.5 and PNaSS transparent particles with a weight average molecular weight of 1×10 6 g / mol and a polydispersity of 3.4 were obtained. Dissolve the two kinds of polymer transparent particles in deionized water to prepare 0.095-0.125mol / L solution, slowly drop the equal volume of the two solutions into 250mL deionized water, s...

Embodiment 2

[0057] 1. Preparation of Grid Scaffolds

[0058] (1) Preparation of printing materials

[0059] Sodium p-styrenesulfonate (NaSS, anionic monomer) and 3-(methacryloylamino)propyltrimethylammonium chloride (MPTC, cationic monomer) were added to 0.05mol% of 2-ketopentadiene Acid (photoinitiator), at 365nm wavelength, 7.5mW / cm2Ultraviolet light irradiation for 8 hours, prepared 1mol / L anion precursor aqueous solution and cationic precursor aqueous solution respectively. It was then precipitated by adding ethanol, and the precipitate was dried in an oven. PMPTC transparent particles with a weight average molecular weight of 4×10 3 g / mol and a polydispersity of 1.5 and PNaSS transparent particles with a weight average molecular weight of 1×10 6 g / mol and a polydispersity of 3.4 were obtained. Dissolve the two kinds of polymer transparent particles in deionized water to prepare 0.095-0.125mol / L solution, slowly drop the equal volume of the two solutions into 250mL deionized water, ...

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Abstract

The invention discloses a tissue-engineered bone based on a multilayer cell grid and a preparation method thereof. The tissue-engineered bone is a mesenchymal stem cell scaffold with a three-dimensional capillary network, and the scaffold is made of a polyionic compound PIC material through The three-dimensional printed three-dimensional mesh is alternately filled with methacrylate gelatin that encapsulates mesenchymal stem cells and incorporates matrigel, and methacrylate that encapsulates vascular endothelial cells and incorporates matrigel gelatin. While inducing the mesenchymal stem cells to form the biological bone tissue, the present invention can promote the formation of the capillary network in the mesenchymal stem cells, and finally form the biological bone tissue scaffold with the three-dimensional capillary network outside the body. The scaffold material has excellent toughness and resilience. After being implanted in the body, the material can be fixed on the bone defect under tension, so that the implant can be used to repair the defect while achieving the effect of stretching the autogenous bone to form bone, and improving the quality of the bone. Defect repair effect.

Description

technical field [0001] The invention relates to a tissue-engineered bone and a preparation method thereof, in particular to a tissue-engineered bone composed of multilayered cell grids obtained by a three-dimensional printing technique and a preparation method thereof, belonging to the field of tissue engineering. Background technique [0002] Since bone tissue defects and loss caused by congenital malformations, trauma, tumors, etc. are common clinical diseases, the functional reconstruction and repair of bone tissue defects has always been an important topic in regenerative medicine. Traditional treatment methods often use autologous or allogeneic bone transplantation, but its clinical application is often limited due to limited supply sources or immune rejection. Tissue engineering is the most widely used artificial bone substitute material. It is currently an effective method to prepare hydrogel scaffolds containing cells. Due to its good biocompatibility and other cha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/52A61L27/50A61L27/54A61L27/16B33Y10/00B33Y70/00B33Y80/00
CPCA61L27/16A61L27/3808A61L27/3834A61L27/3847A61L27/3886A61L27/50A61L27/52A61L27/54A61L2300/412A61L2400/12A61L2430/02B33Y10/00B33Y70/00B33Y80/00C08L25/18
Inventor 俞梦飞王慧明尹俊叶鑫游东奇王少龙
Owner ZHEJIANG UNIV
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