Soilless cultivation oxygen-carrier deep-flow culture method

Abstract

The present invention discloses a soilless cultivation oxygen-carrier deep-flow culture method. The soilless cultivation oxygen-carrier deep-flow culture method comprises the following steps: A, taking an emulsifier and a small amount of water, conducting full stirring and slowly adding an oxygen carrier during the stirring to form an O / W type emulsion; B, slowly adding the mixed emulsifier and oxygen carrier emulsion to a conventional culture solution under stirring; and C, adding the oxygen carrier to the conventional culture solution, wherein the amount of the oxygen carrier is 1-20% of theconventional culture solution, selecting n-hexane, n-dodecane, soybean oil and toluene, and adding 0.1-5% of a fat-soluble microorganism-inhibiting agent. The oxygen supply method increases oxygen supply time through, enables traditional culture fluid flow of 15 minutes every hour to a culture fluid flow of 15 minutes every 3-6 hours, enables local oxygen supply to be more uniform, is beneficialfor the root system to quickly obtain nutrients, increases stress resistance of soilless cultivation, increases yield and reduces energy consumption.

Description

technical field [0001] The invention relates to the technical field of soilless cultivation, in particular to a soilless cultivation oxygen carrier deep liquid flow cultivation method. Background technique [0002] Soilless cultivation is divided into four types: film flow, spray cultivation, deep liquid flow cultivation and still water flower cultivation. Among the four modes, the important basis for their classification is the supply of oxygen to the root system. Compared with soil cultivation, hydroponics is the Speaking, the most important difference is the oxygen supply method of the root system, so it becomes very important to meet the functional supply of oxygen to the root. [0003] Oxygen carrier artificial red blood cells, modified hemoglobin solutions, perfluorocarbon emulsions, and oxygen-dissolving organic solvents such as n-hexane, n-dodecane, soybean oil, and toluene have greatly improved the solubility of oxygen. Oxygen carriers have been used in fermentatio...

AI Technical Summary

Problems solved by technology

Oxygen carriers have been used in fermentation, called biphasic liquid fermentation, such as (CN103820520B) a fermentation method for high-yield natural astaxanthin. In this patent, oxygen carriers are simply added. Although the efficiency of dissolved oxygen is improved, its There is still room for improvement

[0004] The efficiency of oxygen transfer is determined by the contact area of ​​the two media. The efficiency of oxygen transfer to water is related to the contact degree of the gas-liquid interface. Similarly, in the process of oxygen carrier-mediated dissolved oxygen transfer, it depends on the Transfer efficiency, the contact between air and oxygen carrier or air and water, and oxygen carrier and water, because the oxygen carrier is insoluble in water, so to increase the contact with water, it is best to turn the oxygen carrier into an emulsified Liquid, if the oxygen carrier can be dispersed into small droplets with a diameter of about one micron, its contact area with water will reach more than 300 times the original, and the increase in the contact area will certainly increase the transfer efficiency of oxygen, but If an emulsion is formed, it is better that the density of the oxygen carrier is comparable to the water density, because the density of perfluorocarbons is 1.8-2.5 times the water density, while others such as oxygen carrier artificial red blood cells, modified hemoglobin solutions and Oxygen-dissolving organic solvents such as n-hexane, n-dodecane, soybean oil, toluene, etc., their density is basically between 0.85-1.1 times the water density, so they are suitable as oxygen carriers that can form emulsions, and they are used for soilless cultivation The root system culture of soilless culture will have a great impact on the dissolved oxygen of the root system of soilless culture, and then affect the transformation of the culture method. Therefore, we propose a soilless culture oxygen carrier deep liquid flow culture method