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Application of m5C methylation-related regulatory genes in prognosis prediction of hepatocellular carcinoma

A technology related to regulation and prognosis of liver cancer, applied in the field of biomedicine, can solve the problems of unknown diagnostic and prognostic value

Pending Publication Date: 2020-05-22
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, little is known about the relationship between m5C methylation-related regulatory genes and HCC, and the diagnostic and prognostic predictive value of m5C methylation-related regulatory genes for HCC remains unknown

Method used

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  • Application of m5C methylation-related regulatory genes in prognosis prediction of hepatocellular carcinoma
  • Application of m5C methylation-related regulatory genes in prognosis prediction of hepatocellular carcinoma
  • Application of m5C methylation-related regulatory genes in prognosis prediction of hepatocellular carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Effect of m5C methylation-related regulatory gene CNV on the expression of m5C methylation-related regulatory genes

[0039] Firstly, the effect of CNV on the expression of m5C methylation-related regulatory genes was explored. By analyzing the transcriptome data of 423 HCC samples, 11 m5C methylation-related regulatory genes were detected, and the 11 related regulatory genes were NSUN2, NSUN3, NSUN4, NSUN5, NSUN6, NSUN7, DNMT1, DNMT3A, DNMT3B, TET2 and ALYREF. In order to explore the relationship between the expression of the above 11 m5C methylation-related regulatory genes and CNV, we conducted a t-test between different types of CNV and gene expression, and the results are as follows: figure 1 shown. Depend on figure 1 It can be seen that CNV affects the mRNA expression of 9 genes (9 genes are respectively NSUN2, NSUN3, NSUN4, NSUN5, NSUN6, NSUN7, DNMT3A, TET2 and ALYREF) (such as figure 1 A~I in ), however, CNV had no significant difference in the mR...

Embodiment 2

[0040] Example 2: Analysis of the relationship between the expression of m5C methylation-related regulatory genes in hepatocellular carcinoma and the clinical TNM stage of HCC

[0041] In order to further study the expression of m5C methylation-related regulatory genes in different HCC clinical TNM stages, the present invention analyzes the relationship between the expression of m5C methylation-related regulatory genes and HCC clinical TNM stages on the HCC samples obtained from the TCGA database. , in order to facilitate statistical analysis, the clinical TNM stages of HCC are classified as low stage (Low stage), and the HCC clinical TNM stages of stage III and IV are classified as high stage (High stage). Analysis results such as figure 2 shown. Depend on figure 2 It can be seen that the expression of DNMT3A, NSUN4, NSUN5, DNMT1, TET2 and ALYREF genes in high-stage liver cancer tissues is significantly higher than that in low-stage tissues (for details, see figure 2 A ...

Embodiment 3

[0042] Example 3: Relationship between the expression of m5C methylation-related regulatory genes and the prognosis of hepatocellular carcinoma

[0043] In order to further study the relationship between the expression of m5C methylation-related regulatory genes and the prognosis of hepatocellular carcinoma, 319 HCC samples obtained from the TCGA database were analyzed for the relationship between clinical TNM stage and HCC prognosis, and the expression of m5C methylation-related regulatory genes was analyzed. Analysis of the relationship with the prognosis of HCC, the analysis results are as follows image 3 shown. Depend on image 3 In A and B, it can be seen that there is a significant correlation between the clinical TNM stage of HCC and the prognosis of patients. Therefore, CNV and SNV (single nucleotide variation, single nucleotide variation) were used as research objects in the follow-up to further analyze the 11 m5C methylation-related regulatory genes (NSUN2, NSUN3,...

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Abstract

The invention belongs to the technical field of biomedicine, and specifically discloses an application of m5C methylation-related regulatory genes in prognosis prediction of hepatocellular carcinoma.The m5C methylation-related regulatory genes include a NSUN4 gene and an ALYREF gene, and reagents for detecting the expression levels of the NSUN4 gene and the ALYREF gene can be used to prepare prognostic prediction products of the hepatocellular carcinoma. By detecting the expression levels of the NSUN4 gene and the ALYREF gene, the survival time of hepatocellular carcinoma patients can be effectively distinguished, so that a novel way is provided for the prognosis prediction of the hepatocellular carcinoma, and a reference basis is provided for a clinician to analyze the condition of the hepatocellular carcinoma.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of m5C methylation-related regulatory genes in the prognosis prediction of liver cancer. Background technique [0002] Hepatocellular carcinoma (HCC) is the most common disease among adult liver malignancies, ranks third among the most common causes of cancer-related death worldwide (accounting for 8.2% of all cases), and is a serious public health problem . Although its treatment strategy has been greatly developed, the five-year survival rate of advanced HCC is still low due to the late diagnosis of the cancer, easy metastasis and frequent recurrence after surgery. Therefore, studying the molecular mechanism of HCC initiation and progression is crucial for the identification of novel biomarkers and effective therapeutic targets for early detection. [0003] Accumulating evidence shows that post-transcriptional modification of RNA plays a crucial role in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/154C12Q2600/158
Inventor 何玉婷郭文治于潇郑清元张起尧
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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