Test strip for detecting lactic acid and preparation method thereof

A test strip and lactic acid technology, applied in the field of detection, can solve the problems of unsuitable detection, poor accuracy, and high sensitivity of electrochemical methods, and achieve the effects of convenient even detection and simple use.

Inactive Publication Date: 2020-05-19
杭州联晟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3. Myasthenia gravis
The chemical method is complex, takes a long time, and has poor accuracy; the electrochemical method has high sensitivity, wide linear range, and fast speed, but the equipment is too large, expensive, and the operation is complicated, so it is not suitable for routine laboratory and outdoor detection.
The liquid reagent enzyme reaction method adopts enzyme catalysis, which has high sensitivity and wide linear range. It is used for the detection of automatic biochemical analyzers or blood gas analyzers, but the operation is relatively complicated, and the reagent consumables are relatively expensive, and it is not suitable for grassroots or outdoor detection.

Method used

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  • Test strip for detecting lactic acid and preparation method thereof
  • Test strip for detecting lactic acid and preparation method thereof
  • Test strip for detecting lactic acid and preparation method thereof

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preparation example Construction

[0041] A preparation method for a test strip for detecting lactic acid, comprising the steps of:

[0042] Step 1, prepare the materials according to the following formula and make the reaction solution:

[0043] The formula includes: 5-50KU / L lactate oxidase, 5-50KU / L pyruvate oxidase, 5-50KU / L horseradish peroxidase, 5-50KU / L ascorbate oxidase, 1-4 parts Film-forming agent, 2.6-7.5 parts of stabilizer, 0.1-0.5 parts of surfactant, 1.02-4 parts of color-developing substance; these materials are added to the buffer solution and stirred to obtain a reaction solution for use;

[0044] Step 2, making the reaction membrane 201:

[0045] Soaking the reaction membrane 201 in the reaction solution, taking it out, drying it for use;

[0046] Step 3: Compress the bottom plate 100, the processed reaction membrane 201, the blood filter membrane 202, the double-sided adhesive tape 200, the diffusion membrane 300, the U-shaped adhesive tape 400, and the hydrophilic membrane 500 respective...

Embodiment 1

[0049] A kind of test strip for detecting lactic acid, its manufacture method comprises the following steps

[0050] 1. Step one: preparation reagent A: 10KU lactate oxidase, 25KU pyruvate oxidase, 25KU ascorbate oxidase, 25KU horseradish peroxidase, 2g polyvinylpyrrolidone, 2g bovine serum albumin, 3g seaweed Sugar, 2g magnesium chloride (stabilizer), 0.5g Proclin300 (stabilizer), 0.1g tween-20, 0.5g N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethyl Aniline sodium salt, 2g of 4-aminoantipyrine was added to 100mL of 0.1M phosphate buffer with a pH value of 7.0 and stirred for one hour before use;

[0051] 2. Step two: production of reaction membrane 201: soak the reaction membrane 201 in reagent A for 5 minutes, take it out, dry it at 45 degrees for 30 minutes, and then use it;

[0052] 3. Step 3: Press Attach figure 1 And attached figure 2 In this way, the bottom plate 100, the processed reaction membrane 201, the blood filter membrane 202, the double-sided adhesive tape 2...

Embodiment 2

[0057] A kind of test strip for detecting lactic acid, its manufacture method comprises the following steps

[0058] 1. Step one: preparation reagent A: 5KU lactate oxidase, 10KU pyruvate oxidase, 5KU ascorbate oxidase, 10KU horseradish peroxidase, 1g polyvinylpyrrolidone, 0.5g bovine serum albumin, 1g Trehalose, 1g magnesium chloride, 0.1g Proclin300, 0.1g tween-20, 0.02g N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt, 1g 4-amino Antipyrine was added to 100mL of 0.1M phosphate buffer with a pH value of 6.4 and stirred for one hour before use;

[0059] 2. Step two: production of reaction membrane 201: soak the reaction membrane 201 in reagent A for 5 minutes, take it out, dry it at 45 degrees for 30 minutes, and then use it;

[0060] 3. Step 3: Press Attach figure 1 And attached figure 2 In this way, the bottom plate 100, the processed reaction membrane 201, the blood filter membrane 202, the double-sided adhesive tape 200, the diffusion membrane 300, ...

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Abstract

The invention discloses a test strip for detecting lactic acid and a preparation method thereof. The test strip comprises a lactic acid reaction system, a first fixing layer formed on the lactic acidreaction system and a hydrophilic film covering the first fixing layer, wherein the lactic acid reaction system comprises a diffusion film formed between the U-shaped glue, a blood filtering film arranged below the diffusion film, a reaction film arranged below the blood filtering film, a second fixing layer which is arranged below the first fixing layer and used for fixing the blood filtering film and the reaction film, a bottom plate arranged below the second fixing layer, and a detection hole which is formed in the bottom plate and located below the reaction film. The test strip prepared bythe preparation method is matched with a dry biochemical analyzer to be used so that the content of lactic acid in body fluid in a human body or an animal body can be rapidly and quantitatively detected, and the detected result is well matched with the hospital inspection result.

Description

technical field [0001] The invention relates to the detection field, in particular to a test strip for detecting lactic acid and a preparation method thereof. Background technique [0002] Lactic acid is a product of the human body's metabolic process, and its English abbreviation is Lac. The reduction of blood perfusion in various tissues of the body due to functional or organic lesions, or tissue hypoxia caused by hypoxemia can cause blood lactic acid raised. Attention should be paid to the determination of lactic acid: draw blood on an empty stomach and in a resting state; do not use a tourniquet when drawing blood, and do not clench your fist hard. Elevated lactic acid can be seen in the following diseases: 1. Shock, cardiac decompensation, blood disease, pulmonary insufficiency. 2. Liver cirrhosis and advanced liver cancer. 3. Myasthenia gravis. 4. In diabetic coma without ketosis, in the terminal stage of various diseases, lactic acid can reach 25mmol / L or even hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 陈军刚
Owner 杭州联晟生物科技有限公司
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