Novel method for extracting stevioside efficiently and quickly

Abstract

The invention relates to a novel method for extracting stevioside efficiently and quickly. The novel method comprises the following steps of (1) drying stevia rebaudiana leaves, performing crushing, and performing screening with a 100-200-mesh sieve; (2) then performing soaking in petroleum ether under the stirring condition of 50-70 DEG C for 30-45min; (3) after filtration, performing leaching treatment on filtered substances in a sodium citrate solution which contains complex enzymes and of which the pH is 4-5, wherein the complex enzymes are cellulase / hemicellulase / pectinase and chitosan enzymes, the proportion of the four complex enzymes is (4-5) to (3-4) to (2-1) to (1-0.5), the ratio of the filtered substances (by weight) to a solvent namely water is 1 to (8-10), the content of the complex enzymes in a leaching solution is 0.5%-0.8%, the leaching temperature is 20-55 DEG C, leaching is performed for three times, the leaching time each time is 10-20min, an enzyme adding manner isa step progressively-decreased adding manner, during leaching, leaching equipment is used for performing leaching under high pressure, the intensity of pressure is set to be 200-350Mpa, during leaching, ultrasonic strengthening is adopted, the ultrasonic power is 400-500W, the frequency is 100-2000Hz, cold air is charged into the leaching solution, the flow of the cold air is calculated by using each volume of solutions to be extracted as the standard, and 100-300cm<3> / min is needed; and (4) adding a flocculant, performing a flocculation reaction, then performing filtration, performing decolorization, performing concentration and performing crystallization.

Description

technical field [0001] The invention relates to the extraction of food additives, in particular to a method for efficiently and continuously extracting steviol glycosides from stevia leaves. Background technique [0002] Steviol glycoside is the third largest natural sugar source after cane sugar and beet sugar, and also the first largest low-calorie natural sugar source. Its sweetness is 150-300 times that of sucrose, and its calories are only 1 / of that of sucrose. 300, is an ideal low-calorie natural sugar source. Steviol glycosides are extracted and processed from stevia leaves. Stevia is easy to grow, has strong adaptability and rich sources, and is a promising economic crop. Stevia leaf planting originated in Paraguay, South America, and was successfully introduced from Japan to China. China is now the world's largest planter of stevia, and also the largest producer and exporter of steviol glycosides. [0003] From the 1970s and 1980s to the present, the extraction...

AI Technical Summary

Problems solved by technology

[0007] The disadvantages of the above-mentioned conventional production process for extracting steviol glycosides are: many processes, long production process, long cycle, large floor area, many equipments, discontinuous production, low degree of automation, and some processes realize stage mechanized production, loss Large, low productivity and manufacturing rate, high production cost

Its likewise enzyme use single, as two common enzyme use

It still takes longer to extract, leading to a risk of spoilage

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